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AB112572

Anti-ATAD3A antibody

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(4 Publications)

Rabbit Polyclonal ATAD3A antibody. Suitable for ICC, WB, ICC/IF and reacts with Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human ATAD3A.

View Alternative Names

ATPase family AAA domain-containing protein 3A, ATAD3A

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-ATAD3A antibody (AB112572)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-ATAD3A antibody (AB112572)

Immunofluorescence of ATAD3A in Daudi cells with ab112572 at 20 ug/mL.

Immunocytochemistry - Anti-ATAD3A antibody (AB112572)
  • ICC

Unknown

Immunocytochemistry - Anti-ATAD3A antibody (AB112572)

Immunocytochemistry of ATAD3A in Daudi cells with ab112572 at 20 ug/mL.

Western blot - Anti-ATAD3A antibody (AB112572)
  • WB

Unknown

Western blot - Anti-ATAD3A antibody (AB112572)

All lanes:

Western blot - Anti-ATAD3A antibody (ab112572) at 1 µg/mL

All lanes:

Daudi cell lysate at 15 µg

Predicted band size: 71 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB, ICC, ICC/IF

applications

Immunogen

Synthetic Peptide within Human ATAD3A. The exact immunogen used to generate this antibody is proprietary information.

Q9NVI7

Specificity

ab112572 is predicted to not cross-react with other ATAD protein family members.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "20 µg/mL", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
ab112572 is purified by affinity chromatography purified via peptide column.
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATAD3A also known as ATPase family AAA domain-containing protein 3A functions as a mitochondrial membrane protein with an approximate molecular mass of 67 kDa. This protein is expressed widely in human tissues with high levels detected in the brain heart and liver. Mechanically ATAD3A facilitates interactions between the inner and outer mitochondrial membranes playing a role in mitochondrial dynamics and integrity.
Biological function summary

The maintenance of mitochondrial structure and function heavily involves ATAD3A. It forms part of a multiprotein complex that ensures the proper organization of mitochondrial nucleoids thereby regulating mitochondrial DNA replication and transcription. It also participates in maintaining the stability and distribution of mitochondrial proteins contributing to the overall energy metabolism of the cell.

Pathways

ATAD3A engages in key processes related to mitochondrial biogenesis and dynamics. Involvement in pathways like the mitochondrial DNA replication pathway and the energy production pathway highlights its role in maintaining cellular homeostasis. The protein interacts with others including DRP1 and MFN2 essential proteins that regulate mitochondrial fission and fusion processes contributing to its role in energy metabolism.

ATAD3A has associations with several conditions affecting mitochondrial function. Mutations or dysregulation in ATAD3A contribute to neurodegenerative disorders and certain types of cancer. It has been linked to Parkinson's disease due to its influence on mitochondrial homeostasis and is connected with Alzheimer's disease through its interactions with proteins like PINK1 another critical player in mitochondrial maintenance and function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Essential for mitochondrial network organization, mitochondrial metabolism and cell growth at organism and cellular level (PubMed : 17210950, PubMed : 20154147, PubMed : 22453275, PubMed : 31522117, PubMed : 37832546, PubMed : 39116259). May play an important role in mitochondrial protein synthesis (PubMed : 22453275). May also participate in mitochondrial DNA replication (PubMed : 17210950). May bind to mitochondrial DNA D-loops and contribute to nucleoid stability (PubMed : 17210950). Required for enhanced channeling of cholesterol for hormone-dependent steroidogenesis (PubMed : 22453275). Involved in mitochondrial-mediated antiviral innate immunity (PubMed : 31522117). Required to protect mitochondria from the PERK-mediated unfolded protein response : specifically inhibits the activity of EIF2AK3/PERK at mitochondria-endoplasmic reticulum contact sites, thereby providing a safe haven for mitochondrial protein translation during endoplasmic reticulum stress (PubMed : 39116259). Ability to inhibit EIF2AK3/PERK is independent of its ATPase activity (PubMed : 39116259). Also involved in the mitochondrial DNA damage response by promoting signaling between damaged genomes and the mitochondrial membrane, leading to activation of the integrated stress response (ISR) (PubMed : 37832546).
See full target information ATAD3A
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 13:1121 PubMed35236834

2022

ATAD3A oligomerization promotes neuropathology and cognitive deficits in Alzheimer's disease models.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Zhao,Di Hu,Rihua Wang,Xiaoyan Sun,Philip Ropelewski,Zita Hubler,Kathleen Lundberg,Quanqiu Wang,Drew J Adams,Rong Xu,Xin Qi

Oncology letters 22:706 PubMed34457061

2021

Proteome profiling of formalin-fixed, paraffin-embedded lung adenocarcinoma tissues using a tandem mass tag-based quantitative proteomics approach.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Xie,Dan Wang,Xiao Luo,Zhen Li,Aixia Hu,Hui Yang,Jinxing Tang,Peiyu Gao,Tingyi Sun,Lingfei Kong

Nature communications 10:1371 PubMed30914652

2019

ATAD3A oligomerization causes neurodegeneration by coupling mitochondrial fragmentation and bioenergetics defects.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Zhao,Xiaoyan Sun,Di Hu,Domenick A Prosdocimo,Charles Hoppel,Mukesh K Jain,Rajesh Ramachandran,Xin Qi

Journal of proteome research 13:1502-14 PubMed24552547

2014

Alterations in the rat serum proteome induced by prepubertal exposure to bisphenol a and genistein.

Applications

Unspecified application

Species

Unspecified reactive species

Angela Betancourt,James A Mobley,Jun Wang,Sarah Jenkins,Dongquan Chen,Kyoko Kojima,Jose Russo,Coral A Lamartiniere
View all publications
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Product promise

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