Anti-Ataxin 1 antibody [EPR19613] - BSA and Azide free

• WB

Supplier Data

Western blot - Anti-Ataxin 1 antibody [EPR19613] - BSA and Azide free (AB225895)

Blocking/Dilution buffer : 5% NFDM/TBST.

Exposure time : Lane 1,2,6 : 3 minutes; Lane 3-5 : 15 seconds.

The molecular weight observed is consistent with what has been described in the literature (PMID : 7647801).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab201037).

Lanes 1 - 5:

Western blot - Anti-Ataxin 1 antibody [EPR19613] (<a href='/en-us/products/primary-antibodies/ataxin-1-antibody-epr19613-ab201037'>ab201037</a>) at 1/1000 dilution

Lane 6:

Western blot - Anti-Ataxin 1 antibody [EPR19613] (<a href='/en-us/products/primary-antibodies/ataxin-1-antibody-epr19613-ab201037'>ab201037</a>) at 1/10000 dilution

Lane 1:

SH-SY5Y (human neuroblastoma cell line from bone marrow), whole cell lysate at 20 µg

Lane 2:

HeLa (human epithelial cell line from cervix adenocarcinoma), whole cell lysate at 20 µg

Lane 3:

Neuro-2a (mouse neuroblastoma cell line), whole cell lysate at 20 µg

Lane 4:

Human cerebellum tissue lysate at 20 µg

Lane 5:

Human fetal brain tissue lysate at 20 µg

Lane 6:

U-87 MG (human glioblastoma-astrocytoma epithelial cell line), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 22 kDa,51 kDa,78 kDa,86 kDa

Observed band size: 105 kDa

true

• IP

Supplier Data

Immunoprecipitation - Anti-Ataxin 1 antibody [EPR19613] - BSA and Azide free (AB225895)

Ataxin 1 was immunoprecipitated from 0.35 mg of human brain lysate with ab201037 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab201037 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

Lane 1 : Human brain lysate 10 μg (Input).

Lane 2 : ab201037 IP in human brain lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab201037 in human brain lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 1 second.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab201037).

All lanes:

Immunoprecipitation - Anti-Ataxin 1 antibody [EPR19613] (<a href='/en-us/products/primary-antibodies/ataxin-1-antibody-epr19613-ab201037'>ab201037</a>)

Predicted band size: 86 kDa

Observed band size: 105 kDa

false

• WB

Lab

Western blot - Anti-Ataxin 1 antibody [EPR19613] - BSA and Azide free (AB225895)

Blocking buffer and concentration : 5% NFDM/TBST Diluting buffer and concentration : 5% NFDM /TBST Ataxin1 is mainly expressed in nuclear (PMID : 23760502, PMID : 9778246, PMID : 21384195 PMID : 32620905). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab201037).

Lanes 1 - 3:

Western blot - Anti-Ataxin 1 antibody [EPR19613] - BSA and Azide free (ab225895) at 1/1000 dilution

Lanes 1 - 3:

Western blot - Anti-Ataxin 1 antibody [EPR19613] (<a href='/en-us/products/primary-antibodies/ataxin-1-antibody-epr19613-ab201037'>ab201037</a>) at 1/1000 dilution

Lane 1:

HeLa (Human cervix adenocarcinoma epithelial cell) nuclear fraction lysate at 20 µg

Lane 2:

HeLa (Human cervix adenocarcinoma epithelial cell) membrane fraction lysate at 20 µg

Lane 3:

HeLa (Human cervix adenocarcinoma epithelial cell) cytoplasm fraction lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 86 kDa

false

Exposure time: 60s

• WB

Lab

Western blot - Anti-Ataxin 1 antibody [EPR19613] - BSA and Azide free (AB225895)

Blocking buffer and concentration : 5% NFDM/TBST Diluting buffer and concentration : 5% NFDM /TBST

Lanes 1 - 2:

Western blot - Anti-Ataxin 1 antibody [EPR19613] - BSA and Azide free (ab225895) at 1/10000 dilution

Lanes 1 - 2:

Western blot - Anti-Ataxin 1 antibody [EPR19613] (<a href='/en-us/products/primary-antibodies/ataxin-1-antibody-epr19613-ab201037'>ab201037</a>) at 1/10000 dilution

Lane 1:

293T (Human embryonic kidney epithelial cell) transfected with an empty vector whole cell lysate at 20 µg

Lane 2:

293T transfected with full length human ATXN1 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 86 kDa

false

Exposure time: 1s