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AB186265

Anti-Ataxin 1 antibody [N76/8]

4

(1 Review)

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(1 Publication)

Mouse Monoclonal Ataxin 1 antibody. Suitable for WB, ICC/IF and reacts with Recombinant fragment - Mouse, Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Mouse Atxn1 aa 150-200.

View Alternative Names

Sca1, Atxn1, Ataxin-1, Spinocerebellar ataxia type 1 protein homolog

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-Ataxin 1 antibody [N76/8] (AB186265)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Ataxin 1 antibody [N76/8] (AB186265)

Immunocytochemistry/Immunofluorescence analysis of 4% Formaldehyde-fixed human SK-N-BE Cells (Human Neuroblastoma cells) labeling Ataxin 1 (green) using ab186265 at 1/100 dilution P for 60 min at RT. Secondary Antibody : Goat Anti-Mouse ATTO 488 at 1 : 200 for 60 min at RT. Counterstain : Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1 : 1000, 1 : 5000 for 60 min at RT, 5 min at RT. Localization : Cytoplasm, Nucleus. Magnification : 60X.

Immunocytochemistry/ Immunofluorescence - Anti-Ataxin 1 antibody [N76/8] (AB186265)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-Ataxin 1 antibody [N76/8] (AB186265)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed Neuroblastoma cells (SH-SY5Y) labeling Ataxin 1 with ab186265 at 1/100 dilution. Secondary antibody : AlexaFluor® 488 at 1/1000 dilution. (A) Hoechst (blue) nuclear stain. (B) Phalloidin-iFluor 647 (red) F-Actin stain. (C) Ataxin 1 Antibody (D) Composite.

Western blot - Anti-Ataxin 1 antibody [N76/8] (AB186265)
  • WB

Supplier Data

Western blot - Anti-Ataxin 1 antibody [N76/8] (AB186265)

All lanes:

Western blot - Anti-Ataxin 1 antibody [N76/8] (ab186265) at 1/200 dilution

All lanes:

Monkey COS-1 cells transfected with Ataxin- 1 at 15 µg

Secondary

All lanes:

Goat Anti-Mouse IgG: HRP at 1:1000 for 1 hour RT

Predicted band size: 86 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

N76/8

Isotype

IgG2b

Carrier free

No

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Mouse Atxn1 aa 150-200. The exact immunogen used to generate this antibody is proprietary information.

P54254

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Recombinant fragment - Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

The clone number has been updated from S76-8 to N76/8, both clone numbers name the same antibody clone.
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.1% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ataxin-1 sometimes referred to as 'ATXN1' is a protein encoded by the ATXN1 gene. It forms a part of the polyglutamine protein family and typically weighs around 85 kDa. Ataxin-1 appears in various tissues with the highest expression levels in the brain particularly in neurons of the cerebellum and other areas involved in coordination and motor control. This protein consists of multiple domains that contribute to its diverse range of functions including a highly conserved AXH domain involved in protein-protein interactions.
Biological function summary

Ataxin-1 plays a critical role in gene expression regulation and is also implicated in RNA binding. It often associates with large protein complexes such as those involved in RNA metabolism and transcriptional regulation. Ataxin-1 contributes to transcriptional repression through its interaction with transcriptional co-repressors and other proteins involved in gene expression control. This interaction helps maintain normal cellular functions critical for neuronal homeostasis and development.

Pathways

Ataxin-1 interacts with various cellular mechanisms important for normal physiological function. It operates in the Notch signaling pathway influencing cell fate decisions during development. Ataxin-1 is functionally linked to proteins such as RBPJ which cooperates in the regulation of gene expression through these pathways. Additionally it has connections to the Wnt signaling pathway affecting processes that govern cell growth and division alongside beta-catenin an important player in the Wnt signaling pathways.

Ataxin-1 has strong associations with neurodegenerative conditions most notably spinocerebellar ataxia type 1 (SCA1). Variations in the ataxin-1 gene lead to expanded polyglutamine tracts which cause protein misfolding and aggregation contributing to neuronal dysfunction and degeneration. The protein also has potential links to Alzheimer's disease where altered ataxin-1 expression and interaction with amyloid precursor protein (APP) may exacerbate disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Chromatin-binding factor that repress Notch signaling in the absence of Notch intracellular domain by acting as a CBF1 corepressor. Binds to the HEY promoter and might assist, along with NCOR2, RBPJ-mediated repression (By similarity). May be involved in RNA metabolism (By similarity). In concert with CIC and ATXN1L, involved in brain development (PubMed : 28288114).
See full target information Atxn1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oncotarget 11:1448-1461 PubMed32363002

2020

Characterization of GRK5 as a novel regulator of rhabdomyosarcoma tumor cell growth and self-renewal.

Applications

Unspecified application

Species

Unspecified reactive species

Thao Pham,Kristin Robinson,Terra Vleeshouwer-Neumann,James E Annis,Eleanor Y Chen
View all publications
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Product promise

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