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AB63376

Anti-Ataxin 1 (phospho S776) antibody

5

(1 Review)

|

(2 Publications)

Rabbit Polyclonal antibody. Suitable for WB and reacts with Human samples. Cited in 2 publications.
1 Images
Western blot - Anti-Ataxin 1 (phospho S776) antibody (AB63376)
  • WB

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Western blot - Anti-Ataxin 1 (phospho S776) antibody (AB63376)

All lanes:

Western blot - Anti-Ataxin 1 (phospho S776) antibody (ab63376) at 1/500 dilution

Lane 1:

HepG2 cell extract treated with Adriamycin (0.5 micromoles, 5hours) at 30 µg

Lane 2:

HepG2 cell extract treated with Adriamycin (0.5 micromoles, 5hours) at 30 µg with immunizing phosphopeptide

Predicted band size: 86 kDa

Observed band size: 87 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/1000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ataxin-1 sometimes referred to as 'ATXN1' is a protein encoded by the ATXN1 gene. It forms a part of the polyglutamine protein family and typically weighs around 85 kDa. Ataxin-1 appears in various tissues with the highest expression levels in the brain particularly in neurons of the cerebellum and other areas involved in coordination and motor control. This protein consists of multiple domains that contribute to its diverse range of functions including a highly conserved AXH domain involved in protein-protein interactions.
Biological function summary

Ataxin-1 plays a critical role in gene expression regulation and is also implicated in RNA binding. It often associates with large protein complexes such as those involved in RNA metabolism and transcriptional regulation. Ataxin-1 contributes to transcriptional repression through its interaction with transcriptional co-repressors and other proteins involved in gene expression control. This interaction helps maintain normal cellular functions critical for neuronal homeostasis and development.

Pathways

Ataxin-1 interacts with various cellular mechanisms important for normal physiological function. It operates in the Notch signaling pathway influencing cell fate decisions during development. Ataxin-1 is functionally linked to proteins such as RBPJ which cooperates in the regulation of gene expression through these pathways. Additionally it has connections to the Wnt signaling pathway affecting processes that govern cell growth and division alongside beta-catenin an important player in the Wnt signaling pathways.

Ataxin-1 has strong associations with neurodegenerative conditions most notably spinocerebellar ataxia type 1 (SCA1). Variations in the ataxin-1 gene lead to expanded polyglutamine tracts which cause protein misfolding and aggregation contributing to neuronal dysfunction and degeneration. The protein also has potential links to Alzheimer's disease where altered ataxin-1 expression and interaction with amyloid precursor protein (APP) may exacerbate disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Proceedings of the National Academy of Sciences of 115:E12407-E12416 PubMed30530649

2018

MTSS1/Src family kinase dysregulation underlies multiple inherited ataxias.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander S Brown,Pratap Meera,Banu Altindag,Ravi Chopra,Emma M Perkins,Sharan Paul,Daniel R Scoles,Eric Tarapore,Jessica Magri,Haoran Huang,Mandy Jackson,Vikram G Shakkottai,Thomas S Otis,Stefan M Pulst,Scott X Atwood,Anthony E Oro

Journal of neurochemistry 114:706-16 PubMed20477910

2010

Dopamine D2 receptor signaling modulates mutant ataxin-1 S776 phosphorylation and aggregation.

Applications

ICC/IF

Species

Human

Scoty M Hearst,Mariper E Lopez,Qingmei Shao,Yong Liu,Parminder J S Vig
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
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