AB278048

Anti-Ataxin 7 antibody [EPR24136-89] - BSA and Azide free

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(0 Publication)

Rabbit Recombinant Monoclonal Ataxin 7 antibody. Carrier free. Suitable for WB and reacts with Mouse samples.

View Alternative Names

Sca7, Atxn7, Ataxin-7, Spinocerebellar ataxia type 7 protein homolog

2 Images

Lab

Western blot - Anti-Ataxin 7 antibody [EPR24136-89] - BSA and Azide free (AB278048)

This data was developed using ab259829, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

WB positive cell line : Neuro-2a (PMID : 25306109)

Exposure time : 125 seconds.

All lanes:

Western blot - Anti-Ataxin 7 antibody [EPR24136-89] (<a href='/en-us/products/primary-antibodies/ataxin-7-antibody-epr24136-89-ab259829'>ab259829</a>) at 1/1000 dilution

All lanes:

Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 95 kDa

Observed band size: 95 kDa

false

Lab

Western blot - Anti-Ataxin 7 antibody [EPR24136-89] - BSA and Azide free (AB278048)

This data was developed using ab259829, the same antibody clone in a different buffer formulation.

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

The MW is consistent to literature (PMID : 22367614).

Negative control : mouse kidney (PMID : 11099453).

Exposure time : 81 seconds.

All lanes:

Western blot - Anti-Ataxin 7 antibody [EPR24136-89] (<a href='/en-us/products/primary-antibodies/ataxin-7-antibody-epr24136-89-ab259829'>ab259829</a>) at 1/1000 dilution

Lane 1:

Mouse brain tissue lysate at 20 µg

Lane 2:

Mouse kidney tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 95 kDa

Observed band size: 95 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24136-89

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

ab278048 is the carrier-free version of ab259829.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Ataxin 7 also known as ATXN7 is a protein encoded by the ATXN7 gene. This protein has a molecular mass of about 104 kDa. It plays a role in transcriptional regulation and is involved in the assembly of multiprotein complexes. ATXN7 is predominantly expressed in the brain especially within regions like the cerebellum but can also be found in other tissues throughout the human body.

Biological function summary

Ataxin 7 functions as a part of the SAGA (Spt-Ada-Gcn5 acetyltransferase) complex a multi-subunit structure that is vital for transcriptional co-activation. It helps regulate gene expression by modulating histone acetylation which in turn influences chromatin structure and accessibility. The protein interacts with various other components of the SAGA complex playing a significant role in ensuring the stability and function of the complex.

Pathways

Ataxin 7 involves itself in the regulation of the Notch signaling pathway which is important for cellular differentiation and proliferation. This protein also plays a role in the ubiquitin-proteasome pathway which regulates protein degradation. Through these pathways Ataxin 7 is related to other proteins such as Notch receptors and ubiquitin ligases linking its function to broader cellular processes.

Mutations or polyglutamine expansions in the ATXN7 gene lead to spinocerebellar ataxia type 7 (SCA7) a neurodegenerative disorder characterized by progressive cerebellar ataxia and retinal degeneration. Ataxin 7 mutations may influence the aggregation of proteins like aggregates of ubiquitinated proteins which are connected to the pathology of SCA7. This highlights the role of Ataxin 7 in maintaining neural and retinal health.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Involved in neurodegeneration. Acts as a component of the STAGA transcription coactivator-HAT complex. Mediates the interaction of STAGA complex with the CRX and is involved in CRX-dependent gene activation (By similarity). Necessary for microtubule cytoskeleton stabilization (By similarity).

See full target information Atxn7

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-Ataxin 7 antibody [EPR24136-89] - BSA and Azide free

Western blot - Anti-Ataxin 7 antibody [EPR24136-89] - BSA and Azide free (AB278048)

Western blot - Anti-Ataxin 7 antibody [EPR24136-89] - BSA and Azide free (AB278048)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise