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Rabbit Recombinant Monoclonal ATE1 antibody. N-terminal. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

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Images

Western blot - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (AB199423), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (AB199423), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (AB199423), expandable thumbnail
  • Western blot - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (AB199423), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (AB199423), expandable thumbnail

Publications

  • Evidence-based complementary and alternative medicine : eCAM 2022:78613382022
    Liquiritin Attenuates Angiotensin II-Induced Cardiomyocyte Hypertrophy via ATE1/TAK1-JNK1/2 Pathway.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Jiajia Mo,Peng Zhou,Zhaoxing Chu,Yan Zhao,Xiang Wang
    PubMed 35341136
  • Molecular cancer research : MCR 19:1441-14532021
    ATE1 Inhibits Liver Cancer Progression through RGS5-Mediated Suppression of Wnt/β-Catenin Signaling.
    Applications:
    Unspecified application
    Reactive species:
    Unspecified reactive species
    Cong Xu,Yi-Ming Li,Bo Sun,Fang-Jing Zhong,Lian-Yue Yang
    PubMed 34158395

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested
Mouse
Tested
Expected
Tested
Rat
Tested
Expected
Tested

Tested
Tested

Species

Mouse

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/1000

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species

Human

Dilution info

1/220

Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Expected
Expected

Species

Mouse, Rat

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Rat

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species

Human

Dilution info

1/100

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

Select an associated product type

3 products for Alternative Version

    1 products for Alternative Product

      Target data

      Function

      Involved in the post-translational conjugation of arginine to the N-terminal aspartate or glutamate of a protein. This arginylation is required for degradation of the protein via the ubiquitin pathway. Does not arginylate cysteine residues (By similarity).

      Alternative names

      Arginyl-tRNA--protein transferase 1, Arginyltransferase 1, R-transferase 1, Arginine-tRNA--protein transferase 1, ATE1

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      Rabbit Recombinant Monoclonal ATE1 antibody. N-terminal. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 3 publications.

      Alternative names

      Arginyl-tRNA--protein transferase 1, Arginyltransferase 1, R-transferase 1, Arginine-tRNA--protein transferase 1, ATE1

      Key facts

      Isotype

      IgG

      Form

      Liquid

      Clonality

      Monoclonal

      Immunogen
      • The exact immunogen used to generate this antibody is proprietary information.
      Clone number

      EPR13667(2)

      Purification technique

      Affinity purification Protein A

      Concentration
      Loading...

      Storage

      Shipped at conditions

      Blue Ice

      Appropriate short-term storage duration

      1-2 weeks

      Appropriate short-term storage conditions

      +4°C

      Appropriate long-term storage conditions

      -20°C

      Aliquoting information

      Upon delivery aliquot

      Storage information

      Avoid freeze / thaw cycle

      Notes

      Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

      This product is a recombinant monoclonal antibody, which offers several advantages including:

      • - High batch-to-batch consistency and reproducibility
      • - Improved sensitivity and specificity
      • - Long-term security of supply
      • - Animal-free batch production

      For more information, read more on recombinant antibodies.

      Supplementary info

      Activity summary

      ATE1 also known as Arginyltransferase 1 is an important enzyme that plays a mechanical role in cellular processes. It is responsible for adding the amino acid arginine to the N-terminus of certain target proteins a modification important for protein stability and function. ATE1 has a molecular mass of approximately 75 kDa. This enzyme is expressed in various tissues with higher expression levels observed in the heart liver and brain indicating its importance in these areas.

      Biological function summary

      ATE1 acts as a regulator in protein degradation by tagging proteins with arginine marking them for ubiquitination and subsequent proteasomal degradation. This function is critical for controlling the protein turnover and maintaining cellular homeostasis. ATE1 functions independently and is not known to be a part of any larger protein complex emphasizing its specific role in post-translational modification of proteins.

      Pathways

      This arginylation process by ATE1 integrates into the ubiquitin-proteasome system a pathway significant for protein catabolism. It plays a role in cellular stress responses linking closely with the protein degradation pathways to control damaged or misfolded proteins. ATE1's activity interacts with proteins such as ubiquitin ligases which assist in the tagging and recognition of proteins for degradation ensuring proper proteostasis.

      Associated diseases and disorders

      ATE1 has been implicated in cardiac hypertrophy and cancer. Altered expression or activity of ATE1 can contribute to the dysregulation of protein turnover in the heart connecting it to cardiac hypertrophy. In cancer abnormal arginylation can affect cellular homeostasis and lead to uncontrollable cell proliferation. ATE1 connects to proteins like N-end rule substrates in the context of these diseases acting as a potential biomarker or therapeutic target for disease intervention.

      Product promise

      We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

      In the unlikely event of one of our products not working as expected, you are covered by our product promise.

      Full details and terms and conditions can be found here:
      Terms & Conditions.

      8 product images

      • Western blot - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423), expandable thumbnail

        Western blot - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423)

        Blocking and diluting buffer 5% NFDM/TBST

        All lanes: Western blot - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423) at 1/1000 dilution

        Lane 1: C6 (Rat glial tumor cells) whole cell lysate at 10 µg

        Lane 2: Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

        Lane 3: PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 10 µg

        Lane 4: NIH/3T3 (Mouse embryo fibroblast cells) whole cell lysate at 10 µg

        Lane 5: Mouse kidney tissue lysate at 10 µg

        Lane 6: Rat kidney tissue lysate at 10 µg

        Lane 7: Rat spleen tissue lysate at 10 µg

        Secondary

        All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

        Developed using the ECL technique.

        Predicted band size: 59 kDa

        Observed band size: 59 kDa

        Exposure time: 3min

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423)

        Immunohistochemical analysis of paraffin-embedded human hepatocellular carcinoma tissue labeling ATE1 with ab199423 at 1/250 followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. Cytoplasmic staining on human hepatocellular carcinoma tissue is observed (Subcellular location: Nucleus and cytoplasm [UniProt]). Counter-stained with hematoxylin.

        Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

        Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      • Flow Cytometry (Intracellular) - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423), expandable thumbnail

        Flow Cytometry (Intracellular) - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423)

        Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling ATE1 with ab199423 at 1/220 dilution (red) compared with a rabbit monoclonal IgG isotype control (black) and a unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody.

      • Western blot - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423), expandable thumbnail

        Western blot - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423)

        Blocking and diluting buffer 5% NFDM/TBST

        All lanes: Western blot - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423) at 1/10000 dilution

        Lane 1: HepG2 (Human liver hepatocellular carcinoma) whole cell lysate at 20 µg

        Lane 2: HeLa (Human epithelial cells from cervix adenocarcinoma ) whole cell lysate at 20 µg

        Lane 3: 293 (Human epithelial cells from embryonic kidney) whole cell lysate at 20 µg

        Secondary

        All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

        Developed using the ECL technique.

        Predicted band size: 59 kDa

        Observed band size: 59 kDa

        Exposure time: 3min

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423)

        Immunohistochemical analysis of paraffin-embedded Mouse liver tissue labeling ATE1 with ab199423 at 1/250 followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. Cytoplasm staining on Mouse liver tissue is observed (Subcellular location: Nucleus and Cytoplasm [UniProt]) . Counter stained with Hematoxylin.
        Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

        Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      • Western blot - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423), expandable thumbnail

        Western blot - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423)

        Blocking and diluting buffer 5% NFDM/TBST

        All lanes: Western blot - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423) at 1/1000 dilution

        Lane 1: Mouse brain tissue lysate at 10 µg

        Lane 2: Mouse spleen tissue lysate at 10 µg

        Secondary

        All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

        Developed using the ECL technique.

        Predicted band size: 59 kDa

        Observed band size: 59 kDa

        Exposure time: 1min

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423), expandable thumbnail

        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423)

        Immunohistochemical analysis of paraffin-embedded Rat kidney tissue labeling ATE1 with ab199423 at 1/250 followed by Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500. Cytoplasm staining on Rat kidney tissue is observed (Subcellular location: Nucleus and Cytoplasm [UniProt]) . Counter stained with Hematoxylin.
        Negative control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/500 dilution.

        Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

      • Western blot - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423), expandable thumbnail

        Western blot - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423)

        Blocking and diluting buffer 5% NFDM/TBST

        All lanes: Western blot - Anti-ATE1 antibody [EPR13667(2)] - N-terminal (ab199423) at 1/10000 dilution

        All lanes: Human fetal liver tissue lysate at 10 µg

        Secondary

        All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

        Developed using the ECL technique.

        Predicted band size: 59 kDa

        Observed band size: 59 kDa

        Exposure time: 10s

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      Product protocols

      For this product, it's our understanding that no specific protocols are required. You can:

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