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AB181569

Anti-ATF1 antibody [EPR17028]

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(9 Publications)

Rabbit Recombinant Monoclonal ATF1 antibody. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 9 publications.

View Alternative Names

Cyclic AMP-dependent transcription factor ATF-1, cAMP-dependent transcription factor ATF-1, Activating transcription factor 1, Protein TREB36, ATF1

9 Images
Flow Cytometry (Intracellular) - Anti-ATF1 antibody [EPR17028] (AB181569)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ATF1 antibody [EPR17028] (AB181569)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling ATF1 with ab181569 at 1/150 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

Immunocytochemistry/ Immunofluorescence - Anti-ATF1 antibody [EPR17028] (AB181569)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ATF1 antibody [EPR17028] (AB181569)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling ATF1 with ab181569 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF1 antibody [EPR17028] (AB181569)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF1 antibody [EPR17028] (AB181569)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling ATF1 with ab181569 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on mouse liver (PMID : 11865068; PMID : 28032861). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

Immunocytochemistry/ Immunofluorescence - Anti-ATF1 antibody [EPR17028] (AB181569)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ATF1 antibody [EPR17028] (AB181569)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryo fibroblast cell line) cells labeling ATF1 with ab181569 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on NIH/3T3 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

Immunoprecipitation - Anti-ATF1 antibody [EPR17028] (AB181569)
  • IP

Supplier Data

Immunoprecipitation - Anti-ATF1 antibody [EPR17028] (AB181569)

ATF1 was immunoprecipitated from 1 mg of mouse kidney lysate with ab181569 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab181569 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Mouse kidney lysate 10 μg (Input).

Lane 2 : ab181569 IP in mouse kidney lysate (+).

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab181569 in mouse kidney lysate (-).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

All lanes:

Immunoprecipitation - Anti-ATF1 antibody [EPR17028] (ab181569)

Predicted band size: 29 kDa

false

Western blot - Anti-ATF1 antibody [EPR17028] (AB181569)
  • WB

Supplier Data

Western blot - Anti-ATF1 antibody [EPR17028] (AB181569)

Blocking/Dilution buffer : 5% NFDM/TBST.

The expression profile/ molecular weight observed is consistent with what has been described in the literature (PMID : 10574952).

All lanes:

Western blot - Anti-ATF1 antibody [EPR17028] (ab181569) at 1/1000 dilution

Lane 1:

Mouse heart lysate at 20 µg

Lane 2:

Mouse kidney lysate at 20 µg

Lane 3:

Rat heart lysate at 20 µg

Lane 4:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 29 kDa

Observed band size: 35 kDa

true

Exposure time: 3min

Western blot - Anti-ATF1 antibody [EPR17028] (AB181569)
  • WB

Supplier Data

Western blot - Anti-ATF1 antibody [EPR17028] (AB181569)

Blocking/Dilution buffer : 5% NFDM/TBST.

ATF1 has been shown to be phosphorylated in cultured cells and hyperphosphorylation in tissues (PMID : 20730097). Treatment of cell lysates with alkaline phosphatase lead to complete dephosphorylation, while we were only able to partial dephosphorylate ATF1 in tissue lysates.

All lanes:

Western blot - Anti-ATF1 antibody [EPR17028] (ab181569) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 25 µg

Lane 2:

NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate treated with alkaline phosphatase for 1 hour at 37°C at 25 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)

Predicted band size: 29 kDa

Observed band size: 35 kDa

true

Exposure time: 1s

Western blot - Anti-ATF1 antibody [EPR17028] (AB181569)
  • WB

Supplier Data

Western blot - Anti-ATF1 antibody [EPR17028] (AB181569)

Exposure time : Lane 1 : 3 minutes; Lane 2 : 30 seconds.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ATF1 antibody [EPR17028] (ab181569) at 1/1000 dilution

Lane 1:

C6 (rat glial tumor cell line) whole cell lysate at 10 µg

Lane 2:

RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 29 kDa

Observed band size: 35 kDa

true

Western blot - Anti-ATF1 antibody [EPR17028] (AB181569)
  • WB

Supplier Data

Western blot - Anti-ATF1 antibody [EPR17028] (AB181569)

Blocking/Dilution buffer : 5% NFDM/TBST.

ATF1 has been shown to be phosphorylated in cultured cells and hyperphosphorylation in tissues (PMID : 20730097). Treatment of cell lysates with alkaline phosphatase lead to complete dephosphorylation, while we were only able to partial dephosphorylate ATF1 in tissue lysates.

All lanes:

Western blot - Anti-ATF1 antibody [EPR17028] (ab181569) at 1/1000 dilution

Lane 1:

Mouse kidney lysate at 2.5 µg

Lane 2:

Mouse kidney lysate treated with alkaline phosphatase for 1 hour at 37°C at 2.5 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 29 kDa

Observed band size: 35 kDa

true

Exposure time: 1s

  • Carrier free

    Anti-ATF1 antibody [EPR17028] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17028

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

Flow Cyt (Intra), IP, WB, ICC/IF, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/150", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100", "IHCP-species-notes": "<p>This antibody is not suitable for human and rat species in IHC application.</p> Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol." }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

'ATF1' also known as activating transcription factor 1 is a protein with a mass of approximately 29 kDa. This protein belongs to the ATF/CREB family of transcription factors and functions as a DNA-binding protein. It plays an important role in regulating gene expression by binding to cAMP response elements (CRE) in the promoter regions of target genes. ATF1 is widely expressed in multiple tissues including the brain and various endocrine organs functioning as an important regulator of cellular responses to environmental stimuli.
Biological function summary

ATF1 participates in the regulation of various cellular processes including proliferation differentiation and response to stress signals. It can form heterodimers with other members of the CREB family such as CREB1 allowing it to regulate diverse gene sets. These complexes enhance the DNA-binding affinity and transcriptional activity directed by CRE sites influencing cellular homeostasis and adaptation. Additionally post-translational modifications such as phosphorylation by protein kinases modulate ATF1 activity and stability.

Pathways

ATF1 integrates into the cAMP signaling pathway and the MAPK/ERK pathway. In the cAMP signaling pathway ATF1 responds to hormonal signals that activate adenylate cyclase resulting in increased levels of cAMP and subsequent activation of Protein Kinase A (PKA). PKA phosphorylates ATF1 increasing its transcriptional activity. In the MAPK/ERK pathway ATF1 links to the activation of mitogen-activated protein kinases which modulate gene expression related to cell growth and survival. This interaction also involves other proteins such as CREB and ATF2 highlighting a network of transcription factors that co-regulate gene expression.

ATF1 has implications in cancer and neurodegenerative diseases. In cancer alterations in ATF1 expression or function can lead to aberrant cell growth and survival often linked to its cooperation with CREB-related proteins. Specifically chromosomal translocations involving ATF1 have been observed in certain sarcomas like clear cell sarcoma where it forms a fusion protein that acts as a potent transcriptional activator. Concerning neurodegenerative diseases ATF1 may play a role in neuronal survival and stress response with its activity being impacted by dysregulated signaling pathways and interacting proteins such as CREB and ATF2 contributing to disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This protein binds the cAMP response element (CRE) (consensus : 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation.
See full target information Cyclic AMP-dependent transcription factor ATF-1
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Publications (9)

Recent publications for all applications. Explore the full list and refine your search

Bioscience trends : PubMed41016794

2025

Neurotropin alleviates Alzheimer's disease pathology by inhibiting FUS-mediated Calhm2 transcription, blocking the Calhm2/EFhd2 interaction, to improve mitochondrial dysfunction-associated microglia polarization.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanlu Huang,Fusheng Wei,Xiaoe Cheng,Yinqi Shi,Ziye Liu,Lingling Ye

NPJ precision oncology 9:137 PubMed40348911

2025

A novel TRKB-activating internal tandem duplication characterizes a new mechanism of receptor tyrosine kinase activation.

Applications

Unspecified application

Species

Unspecified reactive species

Lauren M Brown,Gabor Tax,Pablo Acera Mateos,Antoine de Weck,Steve Foresto,Thomas Robertson,Fatimah Jalud,Pamela Ajuyah,Paulette Barahona,Jie Mao,M Emmy M Dolman,Marie Wong,Chelsea Mayoh,Mark J Cowley,Loretta M S Lau,Teresa Sadras,Paul G Ekert

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2406935 PubMed39582289

2024

mA Reader PRRC2A Promotes Colorectal Cancer Progression via CK1ε-Mediated Activation of WNT and YAP Signaling Pathways.

Applications

Unspecified application

Species

Unspecified reactive species

Xi Wu,Shiyang Wang,Yuwei Pan,Mengzhen Li,Manyu Song,Hanfu Zhang,Min Deng,Xu Yang,Jiuzhi Xu,Shuo Zhang,Jinhua Zhang,Fengchao Wang,Maksim V Plikus,Cong Lv,Lu Yu,Zhengquan Yu

Journal of gynecologic oncology 36:e11 PubMed38991944

2024

ATF1 regulates MAL2 expression through inhibition of miR-630 to mediate the EMT process that promotes cervical cancer cell development and metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Yanming Cao,Yuping Peng,Youqun Tang

PNAS nexus 2:pgad202 PubMed37388922

2023

A PITX2-HTR1B pathway regulates the asymmetric development of female gonads in chickens.

Applications

Unspecified application

Species

Unspecified reactive species

Zhelun Peng,Qiu Man,Lu Meng,Sheng Wang,Hao Cai,Chuansheng Zhang,Xianyao Li,Heng Wang,Guiyu Zhu

Life (Basel, Switzerland) 13: PubMed37240849

2023

Aerobic Exercise Delays Alzheimer's Disease by Regulating Mitochondrial Proteostasis in the Cerebral Cortex and Hippocampus.

Applications

Unspecified application

Species

Unspecified reactive species

Kaiyin Cui,Chaoyang Li,Guoliang Fang

Human cell 33:1142-1154 PubMed32776307

2020

LINC00665/miR-9-5p/ATF1 is a novel axis involved in the progression of colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Xuhong Zhao,Wenhao Weng,Yin Long,Weijie Pan,Zhi Li,Fenyong Sun

Cell biochemistry and function 38:77-86 PubMed31758574

2019

Circ_0001658 promotes the proliferation and metastasis of osteosarcoma cells via regulating miR-382-5p/YB-1 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Lisong Wang,Pengbin Wang,Xiujun Su,Bo Zhao

American journal of human genetics 105:29-47 PubMed31204011

2019

Systematic Functional Interrogation of Genes in GWAS Loci Identified ATF1 as a Key Driver in Colorectal Cancer Modulated by a Promoter-Enhancer Interaction.

Applications

Unspecified application

Species

Unspecified reactive species

Jianbo Tian,Jiang Chang,Jing Gong,Jiao Lou,Mingpeng Fu,Jiaoyuan Li,Juntao Ke,Ying Zhu,Yajie Gong,Yang Yang,Danyi Zou,Xiating Peng,Nan Yang,Shufang Mei,Xiaoyang Wang,Rong Zhong,Kailin Cai,Xiaoping Miao
View all publications
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