JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB240253

Anti-ATF1 antibody [EPR17028] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal ATF1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human, Rat samples. Cited in 1 publication.

View Alternative Names

Cyclic AMP-dependent transcription factor ATF-1, cAMP-dependent transcription factor ATF-1, Activating transcription factor 1, Protein TREB36, ATF1

5 Images
Immunocytochemistry/ Immunofluorescence - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (AB240253)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (AB240253)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling ATF1 with ab181569 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181569).

Flow Cytometry (Intracellular) - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (AB240253)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (AB240253)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling ATF1 with ab181569 at 1/150 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181569).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (AB240253)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (AB240253)

Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling ATF1 with ab181569 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on mouse liver (PMID : 11865068; PMID : 28032861). Counter stained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181569).

Immunocytochemistry/ Immunofluorescence - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (AB240253)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (AB240253)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryo fibroblast cell line) cells labeling ATF1 with ab181569 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on NIH/3T3 cell line.

The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181569).

Immunoprecipitation - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (AB240253)
  • IP

Supplier Data

Immunoprecipitation - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (AB240253)

ATF1 was immunoprecipitated from 1 mg of mouse kidney lysate with ab181569 at 1/50 dilution. Western blot was performed from the immunoprecipitate using ab181569 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Mouse kidney lysate 10 μg (Input).

Lane 2 : ab181569 IP in mouse kidney lysate (+).

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab181569 in mouse kidney lysate (-).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 30 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181569).

All lanes:

Immunoprecipitation - Anti-ATF1 antibody [EPR17028] (<a href='/en-us/products/primary-antibodies/atf1-antibody-epr17028-ab181569'>ab181569</a>)

Predicted band size: 29 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17028

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

ICC/IF, Flow Cyt (Intra), IHC-P, IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p>This antibody is not suitable for human and rat species in IHC application.</p>" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column

Product details

ab240253 is the carrier-free version of ab181569.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

'ATF1' also known as activating transcription factor 1 is a protein with a mass of approximately 29 kDa. This protein belongs to the ATF/CREB family of transcription factors and functions as a DNA-binding protein. It plays an important role in regulating gene expression by binding to cAMP response elements (CRE) in the promoter regions of target genes. ATF1 is widely expressed in multiple tissues including the brain and various endocrine organs functioning as an important regulator of cellular responses to environmental stimuli.
Biological function summary

ATF1 participates in the regulation of various cellular processes including proliferation differentiation and response to stress signals. It can form heterodimers with other members of the CREB family such as CREB1 allowing it to regulate diverse gene sets. These complexes enhance the DNA-binding affinity and transcriptional activity directed by CRE sites influencing cellular homeostasis and adaptation. Additionally post-translational modifications such as phosphorylation by protein kinases modulate ATF1 activity and stability.

Pathways

ATF1 integrates into the cAMP signaling pathway and the MAPK/ERK pathway. In the cAMP signaling pathway ATF1 responds to hormonal signals that activate adenylate cyclase resulting in increased levels of cAMP and subsequent activation of Protein Kinase A (PKA). PKA phosphorylates ATF1 increasing its transcriptional activity. In the MAPK/ERK pathway ATF1 links to the activation of mitogen-activated protein kinases which modulate gene expression related to cell growth and survival. This interaction also involves other proteins such as CREB and ATF2 highlighting a network of transcription factors that co-regulate gene expression.

ATF1 has implications in cancer and neurodegenerative diseases. In cancer alterations in ATF1 expression or function can lead to aberrant cell growth and survival often linked to its cooperation with CREB-related proteins. Specifically chromosomal translocations involving ATF1 have been observed in certain sarcomas like clear cell sarcoma where it forms a fusion protein that acts as a potent transcriptional activator. Concerning neurodegenerative diseases ATF1 may play a role in neuronal survival and stress response with its activity being impacted by dysregulated signaling pathways and interacting proteins such as CREB and ATF2 contributing to disease progression.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

This protein binds the cAMP response element (CRE) (consensus : 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation.
See full target information Cyclic AMP-dependent transcription factor ATF-1
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell 187:3120-3140.e29 PubMed38714197

2024

Mapping the cellular biogeography of human bone marrow niches using single-cell transcriptomics and proteomic imaging.

Applications

Unspecified application

Species

Unspecified reactive species

Shovik Bandyopadhyay,Michael P Duffy,Kyung Jin Ahn,Jonathan H Sussman,Minxing Pang,David Smith,Gwendolyn Duncan,Iris Zhang,Jeffrey Huang,Yulieh Lin,Barbara Xiong,Tamjid Imtiaz,Chia-Hui Chen,Anusha Thadi,Changya Chen,Jason Xu,Melissa Reichart,Zachary Martinez,Caroline Diorio,Chider Chen,Vinodh Pillai,Oraine Snaith,Derek Oldridge,Siddharth Bhattacharyya,Ivan Maillard,Martin Carroll,Charles Nelson,Ling Qin,Kai Tan
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB226812

Anti-ATF1 (phospho S63) antibody

Western blot - Anti-ATF1 (phospho S63) antibody (AB226812)

  • 0
  • 0
Primary Antibodies

AB264316

Anti-ATF1 antibody

Immunoprecipitation - Anti-ATF1 antibody (AB264316)

  • 0
  • 0
Primary Antibodies

AB134104

Anti-ATF1 antibody [EPR1590(2)]

RabMAb|Recombinant

Western blot - Anti-ATF1 antibody [EPR1590(2)] (AB134104)

  • 0
  • 0
Primary Antibodies

AB76085

Anti-ATF1 (phospho S63) antibody [EP1591(2)Y]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF1 (phospho S63) antibody [EP1591(2)Y] (AB76085)

  • 4
  • 1

Complementary Products

Primary Antibodies

AB32096

Anti-CREB (phospho S133) antibody [E113]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Western blot - Anti-CREB (phospho S133) antibody [E113] (AB32096)

  • 4
  • 14
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB22759

Anti-Fatty Acid Synthase antibody

KO Validated

Western blot - Anti-Fatty Acid Synthase antibody (AB22759)

  • 5
  • 14
Primary Antibodies

AB128936

Anti-Syndecan-1 antibody [EPR6454]

20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Syndecan-1 antibody [EPR6454] (AB128936)

  • 5
  • 7

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com