Rabbit Recombinant Monoclonal ATF1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human, Rat samples.
Images
![Immunoprecipitation - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (AB240253), expandable thumbnail](https://content.abcam.com/products/images/atf1-antibody-epr17028-bsa-and-azide-free-ab240253--immunoprecipitation-img5905.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (AB240253)")
![Immunocytochemistry/ Immunofluorescence - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (AB240253), expandable thumbnail](https://content.abcam.com/products/images/atf1-antibody-epr17028-bsa-and-azide-free-ab240253--immunocytochemistry-immunofluorescence-img40144.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (AB240253)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (AB240253), expandable thumbnail](https://content.abcam.com/products/images/atf1-antibody-epr17028-bsa-and-azide-free-ab240253--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img65522.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (AB240253)")
![Flow Cytometry (Intracellular) - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (AB240253), expandable thumbnail](https://content.abcam.com/products/images/atf1-antibody-epr17028-bsa-and-azide-free-ab240253--flow-cytometry-intracellular-img50894.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (AB240253)")
![Immunocytochemistry/ Immunofluorescence - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (AB240253), expandable thumbnail](https://content.abcam.com/products/images/atf1-antibody-epr17028-bsa-and-azide-free-ab240253--immunocytochemistry-immunofluorescence-img24706.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (AB240253)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | WB | ICC/IF | Flow Cyt (Intra) | IHC-P | |
|---|---|---|---|---|---|
| Human | Expected | Expected | Expected | Tested | Expected |
| Mouse | Tested | Expected | Tested | Expected | Tested |
| Rat | Predicted | Expected | Predicted | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes This antibody is not suitable for human and rat species in IHC application. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation.
Alternative names
Cyclic AMP-dependent transcription factor ATF-1, cAMP-dependent transcription factor ATF-1, Activating transcription factor 1, Protein TREB36, ATF1
Recommended products
Rabbit Recombinant Monoclonal ATF1 antibody. Carrier free. Suitable for IP, WB, ICC/IF, Flow Cyt (Intra), IHC-P and reacts with Mouse, Human, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR17028
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab240253 is the carrier-free version of Anti-ATF1 antibody [EPR17028] ab181569.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
'ATF1' also known as activating transcription factor 1 is a protein with a mass of approximately 29 kDa. This protein belongs to the ATF/CREB family of transcription factors and functions as a DNA-binding protein. It plays an important role in regulating gene expression by binding to cAMP response elements (CRE) in the promoter regions of target genes. ATF1 is widely expressed in multiple tissues including the brain and various endocrine organs functioning as an important regulator of cellular responses to environmental stimuli.
- Biological function summary
ATF1 participates in the regulation of various cellular processes including proliferation differentiation and response to stress signals. It can form heterodimers with other members of the CREB family such as CREB1 allowing it to regulate diverse gene sets. These complexes enhance the DNA-binding affinity and transcriptional activity directed by CRE sites influencing cellular homeostasis and adaptation. Additionally post-translational modifications such as phosphorylation by protein kinases modulate ATF1 activity and stability.
- Pathways
ATF1 integrates into the cAMP signaling pathway and the MAPK/ERK pathway. In the cAMP signaling pathway ATF1 responds to hormonal signals that activate adenylate cyclase resulting in increased levels of cAMP and subsequent activation of Protein Kinase A (PKA). PKA phosphorylates ATF1 increasing its transcriptional activity. In the MAPK/ERK pathway ATF1 links to the activation of mitogen-activated protein kinases which modulate gene expression related to cell growth and survival. This interaction also involves other proteins such as CREB and ATF2 highlighting a network of transcription factors that co-regulate gene expression.
- Associated diseases and disorders
ATF1 has implications in cancer and neurodegenerative diseases. In cancer alterations in ATF1 expression or function can lead to aberrant cell growth and survival often linked to its cooperation with CREB-related proteins. Specifically chromosomal translocations involving ATF1 have been observed in certain sarcomas like clear cell sarcoma where it forms a fusion protein that acts as a potent transcriptional activator. Concerning neurodegenerative diseases ATF1 may play a role in neuronal survival and stress response with its activity being impacted by dysregulated signaling pathways and interacting proteins such as CREB and ATF2 contributing to disease progression.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunoprecipitation - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (ab240253), expandable thumbnail](https://content.abcam.com/products/images/atf1-antibody-epr17028-bsa-and-azide-free-ab240253--immunoprecipitation-img5905.jpg "Immunoprecipitation - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (ab240253)")
Immunoprecipitation - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (ab240253)
ATF1 was immunoprecipitated from 1 mg of mouse kidney lysate with Anti-ATF1 antibody [EPR17028] ab181569 at 1/50 dilution. Western blot was performed from the immunoprecipitate using Anti-ATF1 antibody [EPR17028] ab181569 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366), was used for detection at 1/10000 dilution.
Lane 1: Mouse kidney lysate 10 μg (Input).
Lane 2: Anti-ATF1 antibody [EPR17028] ab181569 IP in mouse kidney lysate (+).
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-ATF1 antibody [EPR17028] ab181569 in mouse kidney lysate (-).
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ATF1 antibody [EPR17028] ab181569).
All lanes: Immunoprecipitation - Anti-ATF1 antibody [EPR17028] (Anti-ATF1 antibody [EPR17028] ab181569)
Predicted band size: 29 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (ab240253), expandable thumbnail](https://content.abcam.com/products/images/atf1-antibody-epr17028-bsa-and-azide-free-ab240253--immunocytochemistry-immunofluorescence-img40144.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (ab240253)")
Immunocytochemistry/ Immunofluorescence - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (ab240253)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling ATF1 with Anti-ATF1 antibody [EPR17028] ab181569 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on HeLa cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ATF1 antibody [EPR17028] ab181569).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (ab240253), expandable thumbnail](https://content.abcam.com/products/images/atf1-antibody-epr17028-bsa-and-azide-free-ab240253--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img65522.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (ab240253)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (ab240253)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue labeling ATF1 with Anti-ATF1 antibody [EPR17028] ab181569 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nuclear staining on mouse liver (PMID: 11865068; PMID: 28032861). Counter stained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Perform heat mediated antigen retrieval using Antigen Retrieval Buffer (100X Tris-EDTA Buffer, pH 9.0) ab93684 (Tris/EDTA buffer, pH 9.0).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ATF1 antibody [EPR17028] ab181569).
![Flow Cytometry (Intracellular) - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (ab240253), expandable thumbnail](https://content.abcam.com/products/images/atf1-antibody-epr17028-bsa-and-azide-free-ab240253--flow-cytometry-intracellular-img50894.jpg "Flow Cytometry (Intracellular) - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (ab240253)")
Flow Cytometry (Intracellular) - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (ab240253)
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed, 0.1% Tween-20 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling ATF1 with Anti-ATF1 antibody [EPR17028] ab181569 at 1/150 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ATF1 antibody [EPR17028] ab181569).
![Immunocytochemistry/ Immunofluorescence - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (ab240253), expandable thumbnail](https://content.abcam.com/products/images/atf1-antibody-epr17028-bsa-and-azide-free-ab240253--immunocytochemistry-immunofluorescence-img24706.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (ab240253)")
Immunocytochemistry/ Immunofluorescence - Anti-ATF1 antibody [EPR17028] - BSA and Azide free (ab240253)
Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryo fibroblast cell line) cells labeling ATF1 with Anti-ATF1 antibody [EPR17028] ab181569 at 1/100 dilution followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing nuclear staining on NIH/3T3 cell line.
The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) (red) at 1/200 dilution.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) secondary antibody at 1/1000 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ATF1 antibody [EPR17028] ab181569).
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