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AB76085

Anti-ATF1 (phospho S63) antibody [EP1591(2)Y]

4

(1 Review)

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(10 Publications)

Rabbit Recombinant Monoclonal ATF1 phospho S63 antibody. Suitable for WB, IHC-P and reacts with Human, Rat, Mouse samples. Cited in 10 publications.

View Alternative Names

Cyclic AMP-dependent transcription factor ATF-1, cAMP-dependent transcription factor ATF-1, Activating transcription factor 1, Protein TREB36, ATF1

6 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF1 (phospho S63) antibody [EP1591(2)Y] (AB76085)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF1 (phospho S63) antibody [EP1591(2)Y] (AB76085)

Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue without alkaline phosphatase treatment (image A)/treated with alkaline phosphatase (image B) labeling ATF1 (phospho S63) with ab76085 at 1/2400 (0.105 ug/ml) dilution.

Positive staining on human breast cancer tissue without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab76085 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF1 (phospho S63) antibody [EP1591(2)Y] (AB76085)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF1 (phospho S63) antibody [EP1591(2)Y] (AB76085)

Immunohistochemical analysis of paraffin-embedded Mouse liver tissue without alkaline phosphatase treatment (image A)/treated with alkaline phosphatase (image B) labeling ATF1 (phospho S63) with ab76085 at 1/2400 (0.105 ug/ml) dilution.

Positive staining on mouse liver tissue without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab76085 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF1 (phospho S63) antibody [EP1591(2)Y] (AB76085)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF1 (phospho S63) antibody [EP1591(2)Y] (AB76085)

Immunohistochemical analysis of paraffin-embedded Rat colon tissue without alkaline phosphatase treatment (image A)/treated with alkaline phosphatase (image B) labeling ATF1 (phospho S63) with ab76085 at 1/2400 (0.105 ug/ml) dilution.

Positive staining on rat colon tissue without alkaline phosphatase treatment (image A). No signal was detected when tissues were treated with alkaline phosphatase (image B). The section was incubated with ab76085 for 30 mins at room temperature.
The immunostaining was performed on a Leica Biosystems BOND® RX instrument

Counterstained with Hematoxylin.

Heat mediated antigen retrieval was performed with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Western blot - Anti-ATF1 (phospho S63) antibody [EP1591(2)Y] (AB76085)
  • WB

Lab

Western blot - Anti-ATF1 (phospho S63) antibody [EP1591(2)Y] (AB76085)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ATF1 (phospho S63) antibody [EP1591(2)Y] (ab76085) at 1/100 dilution

Lane 1:

Untreated HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 15 µg

Lane 2:

HeLa whole cell lysate, the membrane was incubated with phosphatase at 37°C for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 29 kDa

false

Exposure time: 100s

Western blot - Anti-ATF1 (phospho S63) antibody [EP1591(2)Y] (AB76085)
  • WB

Lab

Western blot - Anti-ATF1 (phospho S63) antibody [EP1591(2)Y] (AB76085)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ATF1 (phospho S63) antibody [EP1591(2)Y] (ab76085) at 1/100 dilution

Lane 1:

Untreated PC-12 (Rat adrenal gland pheochromocytoma) whole cell lysate at 15 µg

Lane 2:

PC-12 whole cell lysate, the membrane was incubated with phosphatase at 37°C for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 29 kDa

false

Exposure time: 180s

Western blot - Anti-ATF1 (phospho S63) antibody [EP1591(2)Y] (AB76085)
  • WB

Lab

Western blot - Anti-ATF1 (phospho S63) antibody [EP1591(2)Y] (AB76085)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ATF1 (phospho S63) antibody [EP1591(2)Y] (ab76085) at 1/100 dilution

Lane 1:

Untreated RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) whole cell lysate at 15 µg

Lane 2:

RAW 264.7 whole cell lysate, the membrane was incubated with phosphatase at 37°C for 1 hour at 15 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 29 kDa

false

Exposure time: 40s

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EP1591(2)Y

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2400", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Mouse": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2400", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." }, "Rat": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/2400", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol." } } }
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Product details

Production method for this product has been changed from hybridoma to recombinant on 15th August 2024

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

'ATF1' also known as activating transcription factor 1 is a protein with a mass of approximately 29 kDa. This protein belongs to the ATF/CREB family of transcription factors and functions as a DNA-binding protein. It plays an important role in regulating gene expression by binding to cAMP response elements (CRE) in the promoter regions of target genes. ATF1 is widely expressed in multiple tissues including the brain and various endocrine organs functioning as an important regulator of cellular responses to environmental stimuli.
Biological function summary

ATF1 participates in the regulation of various cellular processes including proliferation differentiation and response to stress signals. It can form heterodimers with other members of the CREB family such as CREB1 allowing it to regulate diverse gene sets. These complexes enhance the DNA-binding affinity and transcriptional activity directed by CRE sites influencing cellular homeostasis and adaptation. Additionally post-translational modifications such as phosphorylation by protein kinases modulate ATF1 activity and stability.

Pathways

ATF1 integrates into the cAMP signaling pathway and the MAPK/ERK pathway. In the cAMP signaling pathway ATF1 responds to hormonal signals that activate adenylate cyclase resulting in increased levels of cAMP and subsequent activation of Protein Kinase A (PKA). PKA phosphorylates ATF1 increasing its transcriptional activity. In the MAPK/ERK pathway ATF1 links to the activation of mitogen-activated protein kinases which modulate gene expression related to cell growth and survival. This interaction also involves other proteins such as CREB and ATF2 highlighting a network of transcription factors that co-regulate gene expression.

ATF1 has implications in cancer and neurodegenerative diseases. In cancer alterations in ATF1 expression or function can lead to aberrant cell growth and survival often linked to its cooperation with CREB-related proteins. Specifically chromosomal translocations involving ATF1 have been observed in certain sarcomas like clear cell sarcoma where it forms a fusion protein that acts as a potent transcriptional activator. Concerning neurodegenerative diseases ATF1 may play a role in neuronal survival and stress response with its activity being impacted by dysregulated signaling pathways and interacting proteins such as CREB and ATF2 contributing to disease progression.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

This protein binds the cAMP response element (CRE) (consensus : 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Binds to the Tax-responsive element (TRE) of HTLV-I. Mediates PKA-induced stimulation of CRE-reporter genes. Represses the expression of FTH1 and other antioxidant detoxification genes. Triggers cell proliferation and transformation.
See full target information Cyclic AMP-dependent transcription factor ATF-1 phospho S63
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Publications (10)

Recent publications for all applications. Explore the full list and refine your search

Signal transduction and targeted therapy 8:275 PubMed37463926

2023

Cancer cell employs a microenvironmental neural signal trans-activating nucleus-mitochondria coordination to acquire stemness.

Applications

Unspecified application

Species

Unspecified reactive species

Bin He,Rui Gao,Shasha Lv,Ailin Chen,Junxiu Huang,Luoxuan Wang,Yunxiu Feng,Jiesi Feng,Bing Liu,Jie Lei,Bing Deng,Bin He,Bai Cui,Fei Peng,Min Yan,Zifeng Wang,Eric W-F Lam,Bilian Jin,Zhiming Shao,Yulong Li,Jianwei Jiao,Xi Wang,Quentin Liu

Cell reports 40:111147 PubMed35926467

2022

AP-1 transcription factor network explains diverse patterns of cellular plasticity in melanoma cells.

Applications

Unspecified application

Species

Unspecified reactive species

Natacha Comandante-Lou,Douglas G Baumann,Mohammad Fallahi-Sichani

Cell reports 29:4620-4631.e4 PubMed31875565

2019

N-Glycanase 1 Transcriptionally Regulates Aquaporins Independent of Its Enzymatic Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Mitali A Tambe,Bobby G Ng,Hudson H Freeze

Oncology reports 41:3355-3366 PubMed30942462

2019

Isobavachalcone exerts anti‑proliferative and pro‑apoptotic effects on human liver cancer cells by targeting the ERKs/RSK2 signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Binbin Li,Nansong Xu,Zheng Wan,Li Ma,Huahui Li,Weijie Cai,Xiumei Chen,Zunnan Huang,Zhiwei He

The Journal of steroid biochemistry and molecular biology 182:50-61 PubMed29684479

2018

Impairment of the Gnα11-controlled expression of claudin-1 and MMP-9 and collective migration of human breast cancer MCF-7 cells by DHEAS.

Applications

Unspecified application

Species

Human

Neha Upmanyu,Ahmed Bulldan,Dimitrios Papadopoulos,Raimund Dietze,Viveka Nand Malviya,Georgios Scheiner-Bobis

PloS one 11:e0150143 PubMed26938869

2016

Dehydroepiandrosterone Sulfate Stimulates Expression of Blood-Testis-Barrier Proteins Claudin-3 and -5 and Tight Junction Formation via a Gnα11-Coupled Receptor in Sertoli Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Dimitrios Papadopoulos,Raimund Dietze,Mazen Shihan,Ulrike Kirch,Georgios Scheiner-Bobis

Journal of cancer prevention 19:199-208 PubMed25337589

2014

Fibroblast and Epidermal Growth Factors Utilize Different Signaling Pathways to Induce Anchorage-independent Cell Transformation in JB6 Cl41 Mouse Skin Epidermal Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Cheol-Jung Lee,Mee-Hyun Lee,Yong-Yeon Cho

Arteriosclerosis, thrombosis, and vascular biology 33:2470-80 PubMed24051143

2013

5'-AMP-activated protein kinase-activating transcription factor 1 cascade modulates human monocyte-derived macrophages to atheroprotective functions in response to heme or metformin.

Applications

Unspecified application

Species

Unspecified reactive species

Xinyi Wan,Yilin Huo,Michael Johns,Emma Piper,Justin C Mason,David Carling,Dorian O Haskard,Joseph J Boyle

Biochimica et biophysica acta 1833:3064-3075 PubMed23988737

2013

Dehydroepiandrosterone sulfate mediates activation of transcription factors CREB and ATF-1 via a Gα11-coupled receptor in the spermatogenic cell line GC-2.

Applications

ICC/IF

Species

Human

Mazen Shihan,Ulrike Kirch,Georgios Scheiner-Bobis

Circulation research 110:20-33 PubMed22052915

2011

Activating transcription factor 1 directs Mhem atheroprotective macrophages through coordinated iron handling and foam cell protection.

Applications

ELISA, IHC-P, WB

Species

Human, Human, Human

Joseph J Boyle,Michael Johns,Theresa Kampfer,Aivi T Nguyen,Laurence Game,Dominik J Schaer,Justin C Mason,Dorian O Haskard
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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