Rabbit Recombinant Monoclonal ATF2 antibody. Carrier free. Suitable for ICC/IF, IP, ChIP, WB, IHC-P, Flow Cyt (Intra) and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
ICC/IF | IP | ChIP | WB | IHC-P | Flow Cyt (Intra) | |
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Human | Tested | Tested | Tested | Tested | Tested | Tested |
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Species Human | Dilution info - | Notes - |
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Species Human | Dilution info - | Notes - |
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Species Human | Dilution info - | Notes - |
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Species Human | Dilution info - | Notes - |
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Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
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Transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro (PubMed:10821277). In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type.
CREB2, CREBP1, CREB2, ATF2, CREBP1, Cyclic AMP-dependent transcription factor ATF-2, cAMP-dependent transcription factor ATF-2, Activating transcription factor 2, Cyclic AMP-responsive element-binding protein 2, HB16, cAMP response element-binding protein CRE-BP1, CREB-2, cAMP-responsive element-binding protein 2
Rabbit Recombinant Monoclonal ATF2 antibody. Carrier free. Suitable for ICC/IF, IP, ChIP, WB, IHC-P, Flow Cyt (Intra) and reacts with Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
E243
Affinity purification Protein A
This antibody recognises ATF2, but does not cross react with other ATF family members.
Blue Ice
+4°C
+4°C
Do Not Freeze
ab247240 is the carrier-free version of Anti-ATF2 antibody [E243] - ChIP Grade ab32160.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
The ATF2 protein also referred to as ATF-2 or activating transcription factor 2 plays a significant role as a transcription factor in cellular processes. It weighs approximately 75 kDa and is expressed in many tissues with higher levels in the brain heart and skeletal muscle. Functionally ATF2 belongs to the leucine zipper family of proteins facilitating its ability to bind DNA and regulate the expression of genes involved in stress responses development and growth.
ATF2 takes part in the regulation of gene expression in response to various stimuli. It often forms a complex with other proteins such as c-Jun when binding to the DNA. This complex then influences the transcription of genes that respond to cellular stress and DNA damage. By phosphorylating specific serine residues cellular kinases activate ATF2 which then translocates to the nucleus where it exerts its function.
ATF2 integrates into the MAPK and JNK signaling cascades which are important for transmitting stress signals from the cell surface to the nucleus. Through these pathways ATF2 interacts with proteins such as JNK and p38 MAPK modulating the transcription of downstream genes that control cell proliferation apoptosis and differentiation. Its role in these pathways positions ATF2 as a critical node where various signaling inputs merge to influence cellular outcomes.
ATF2 has associations with conditions such as cancer and neurological disorders. Aberrant regulation of ATF2 can contribute to oncogenesis by affecting cell cycle control and apoptosis. For example in melanoma altered ATF2 activity is linked to tumor progression and resistance to apoptosis. Additionally in neurological disorders its interaction with proteins like phospho-c-Jun influences neuronal survival and plasticity implicating ATF2 in pathologies related to neurodegeneration and cognitive dysfunction.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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This data was developed using Anti-ATF2 antibody [E243] - ChIP Grade ab32160, the same antibody clone in a different buffer formulation.Chromatin was prepared from Jurkat (TPA and Ionomycin treated or not) cells according to the Abcam X-ChIP protocol. Cells were fixed with 1% formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 5µg of Anti-ATF2 antibody [E243] - ChIP Grade ab32160 (red), and 20µl of protein A/G sepharose beads slurry (10µl of sepharose A beads + 10µl of sepharose G beads). 5μg of rabbit normal IgG was added to the beads control (grey). The immunoprecipitated DNA was quantified by real time PCR (Sybr green approach).
This data was developed using Anti-ATF2 antibody [E243] - ChIP Grade ab32160, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-ATF2 antibody [E243] - ChIP Grade (Anti-ATF2 antibody [E243] - ChIP Grade ab32160) at 1/10000 dilution
All lanes: HeLa cell lysate
Predicted band size: 55 kDa
Observed band size: 70 kDa
This data was developed using Anti-ATF2 antibody [E243] - ChIP Grade ab32160, the same antibody clone in a different buffer formulation.Anti-ATF2 antibody [E243] - ChIP Grade ab32160, at a dilution of 1/250, staining ATF2 in paraffin embedded breast carcinoma tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
This data was developed using Anti-ATF2 antibody [E243] - ChIP Grade ab32160, the same antibody clone in a different buffer formulation.Anti-ATF2 antibody [E243] - ChIP Grade ab32160 (purified) at 1/60 dilution (20 µg/mL) immunoprecipitating ATF2 in HeLa whole cell lysate.
Lane 3 (-): HeLa(Human cervix adenocarcinoma epithelial cell) whole cell lysate 10µgab32160 & HeLa whole cell lysateRabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-ATF2 antibody [E243] - ChIP Grade ab32160 in HeLa whole cell lysate
For western blotting, Anti-ATF2 antibody [E243] - ChIP Grade ab32160 at 1/1000 dilution (2.284 µg/mL) and
bbit TureBlot: Anti-Rabbit IgG HRP was used as the secondary antibody at 1/1500 dilution.
Blocking and diluting buffer: 5% NFDM /TBST .
All lanes: Immunoprecipitation - Anti-ATF2 antibody [E243] - ChIP Grade (Anti-ATF2 antibody [E243] - ChIP Grade ab32160)
Predicted band size: 55 kDa
This data was developed using the same antibody clone in a different buffer formulation (Anti-ATF2 antibody [E243] - ChIP Grade ab32160).
Immunocytochemistry analysis of A549 (human lung carcinoma epithelial cell) labeling ATF2 with purified Anti-ATF2 antibody [E243] - ChIP Grade ab32160 at 1/100 dilution (10 µg/ml). Cells were fixed with 4% Paraformaldehyde and permeabilised with 0.1% tritonX-100. Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.10 µg/ml) was used as counterstain. Nuclei were stained blue with DAPI.
Negative control: PBS instead of the primary antibody.
This data was developed using Anti-ATF2 antibody [E243] - ChIP Grade ab32160, the same antibody clone in a different buffer formulation.
Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling ATF2 (red) with Anti-ATF2 antibody [E243] - ChIP Grade ab32160 at a 1/2000 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
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