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AB239361

Anti-ATF2 antibody [EPR22938-114]

5

(1 Review)

|

(11 Publications)

Rabbit Recombinant Monoclonal ATF2 antibody. Suitable for IP, WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 11 publications.

View Alternative Names

CREB2, CREBP1, ATF2, Cyclic AMP-dependent transcription factor ATF-2, cAMP-dependent transcription factor ATF-2, Activating transcription factor 2, Cyclic AMP-responsive element-binding protein 2, HB16, cAMP response element-binding protein CRE-BP1, CREB-2, cAMP-responsive element-binding protein 2

10 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF2 antibody [EPR22938-114] (AB239361)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF2 antibody [EPR22938-114] (AB239361)

Immunohistochemical analysis of paraffin-embedded human kidney carcinoma tissue labeling ATF2 with ab239361 at 1/250 dilution (1.76 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human kidney carcinoma (PMID : 27377902) is observed. The section was incubated with ab239361 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF2 antibody [EPR22938-114] (AB239361)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF2 antibody [EPR22938-114] (AB239361)

Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling ATF2 with ab239361 at 1/250 dilution (1.76 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on human kidney (PMID : 27377902) is observed. The section was incubated with ab239361 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Flow Cytometry (Intracellular) - Anti-ATF2 antibody [EPR22938-114] (AB239361)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-ATF2 antibody [EPR22938-114] (AB239361)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling ATF2 with ab239361 at 1/40 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF2 antibody [EPR22938-114] (AB239361)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF2 antibody [EPR22938-114] (AB239361)

Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling ATF2 with ab239361 at 1/250 dilution (1.76 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on rat kidney is observed. The section was incubated with ab239361 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Flow Cytometry (Intracellular) - Anti-ATF2 antibody [EPR22938-114] (AB239361)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-ATF2 antibody [EPR22938-114] (AB239361)

Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labeling ATF2 with ab239361 at 1/40 (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF2 antibody [EPR22938-114] (AB239361)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF2 antibody [EPR22938-114] (AB239361)

Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling ATF2 with ab239361 at 1/250 dilution (1.76 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse kidney is observed. The section was incubated with ab239361 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Immunoprecipitation - Anti-ATF2 antibody [EPR22938-114] (AB239361)
  • IP

Lab

Immunoprecipitation - Anti-ATF2 antibody [EPR22938-114] (AB239361)

ATF2 was immunoprecipitated from 0.35 mg NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate with ab239361 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab239361. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/1000 dilution.

Lane 1 : NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate 10μg.

Lane 2 : ab239361 IP in NIH/3T3 whole cell lysate.

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab239361 in NIH/3T3 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 8 seconds

Lysate were made freshly and used in IP test immediately to minimize protein degradation. Incubation time was 2h.

The molecular weight and degraded fragments observed are consistent with what has been described in the literature (PMID : 9488727, PMID : 10207054, PMID : 26901653).

All lanes:

Immunoprecipitation - Anti-ATF2 antibody [EPR22938-114] (ab239361)

Predicted band size: 55 kDa

false

Western blot - Anti-ATF2 antibody [EPR22938-114] (AB239361)
  • WB

Lab

Western blot - Anti-ATF2 antibody [EPR22938-114] (AB239361)

The molecular weight observed is consistent with what has been described in the literature (PMID : 26901653).

Exposure time : 10 seconds.

Blocking and Diluting Buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ATF2 antibody [EPR22938-114] (ab239361) at 1/1000 dilution

Lane 1:

NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg

Lane 2:

RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg

Lane 3:

HEK-293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg

Lane 4:

K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg

Lane 5:

Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 55 kDa

Observed band size: 70 kDa

false

Western blot - Anti-ATF2 antibody [EPR22938-114] (AB239361)
  • WB

Lab

Western blot - Anti-ATF2 antibody [EPR22938-114] (AB239361)

The molecular weight and degraded fragments observed are consistent with what has been described in the literature (PMID : 9488727, 10207054, 26901653). Suggest use freshly made lysate to minimize protein degradation.

Exposure time : 3 minutes.

Blocking and Diluting Buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ATF2 antibody [EPR22938-114] (ab239361) at 1/1000 dilution

Lane 1:

PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg

Lane 2:

Rat spleen tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 55 kDa

Observed band size: 70 kDa

false

Western blot - Anti-ATF2 antibody [EPR22938-114] (AB239361)
  • WB

Lab

Western blot - Anti-ATF2 antibody [EPR22938-114] (AB239361)

The molecular weight observed is consistent with what has been described in the literature (PMID : 26901653).

Exposure time : 3 minutes.

Blocking and Diluting Buffer and concentration : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ATF2 antibody [EPR22938-114] (ab239361) at 1/1000 dilution

All lanes:

Mouse brain tissue lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Predicted band size: 55 kDa

Observed band size: 70 kDa

false

  • Carrier free

    Anti-ATF2 antibody [EPR22938-114] - BSA and Azide free

  • Carrier free

    Anti-ATF2 antibody [EPR22938-114] - BSA and Azide free (Capture)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22938-114

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IP, Flow Cyt (Intra), IHC-P, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "ChIP" : {"fullname" : "ChIP", "shortname":"ChIP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/30", "IP-species-notes": "<p></p>", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/40", "FlowCytIntra-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": "<p></p>" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "ChIP-species-checked": "notRecommended", "ChIP-species-dilution-info": "", "ChIP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "guaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": "<p></p>" } } }
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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ATF2 protein also referred to as ATF-2 or activating transcription factor 2 plays a significant role as a transcription factor in cellular processes. It weighs approximately 75 kDa and is expressed in many tissues with higher levels in the brain heart and skeletal muscle. Functionally ATF2 belongs to the leucine zipper family of proteins facilitating its ability to bind DNA and regulate the expression of genes involved in stress responses development and growth.
Biological function summary

ATF2 takes part in the regulation of gene expression in response to various stimuli. It often forms a complex with other proteins such as c-Jun when binding to the DNA. This complex then influences the transcription of genes that respond to cellular stress and DNA damage. By phosphorylating specific serine residues cellular kinases activate ATF2 which then translocates to the nucleus where it exerts its function.

Pathways

ATF2 integrates into the MAPK and JNK signaling cascades which are important for transmitting stress signals from the cell surface to the nucleus. Through these pathways ATF2 interacts with proteins such as JNK and p38 MAPK modulating the transcription of downstream genes that control cell proliferation apoptosis and differentiation. Its role in these pathways positions ATF2 as a critical node where various signaling inputs merge to influence cellular outcomes.

ATF2 has associations with conditions such as cancer and neurological disorders. Aberrant regulation of ATF2 can contribute to oncogenesis by affecting cell cycle control and apoptosis. For example in melanoma altered ATF2 activity is linked to tumor progression and resistance to apoptosis. Additionally in neurological disorders its interaction with proteins like phospho-c-Jun influences neuronal survival and plasticity implicating ATF2 in pathologies related to neurodegeneration and cognitive dysfunction.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro (PubMed : 10821277). In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type.
See full target information ATF2
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Publications (11)

Recent publications for all applications. Explore the full list and refine your search

BMC cancer 25:305 PubMed39979838

2025

Up-regulation of mA writer METTL14 inhibits tumorigenesis by suppressing glycolysis in colorectal cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Lulu Li,Rong Zhang,Yongsheng Li

Chemical biology & drug design 105:e70050 PubMed39821443

2025

Curcumin Regulates Microglia Polarization to Alleviate Ischemic Stroke by Targeting microRNA-205-5p/Kruppel-Like Factor 2 (KLF2)/Activating Transcription Factor 2 (ATF2) Axis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiangyu Cao,Yingzi Pu

Amino acids 56:45 PubMed39007996

2024

The short peptide encoded by long non-coding RNA RNF217-AS1 inhibits stomach cancer tumorigenesis, macrophage recruitment, and pro-inflammatory responses.

Applications

Unspecified application

Species

Unspecified reactive species

Qi Ma,Fei Ma,Bin Zhang,Yonglei Zhang,Liangqun Peng,Xiangnan Li

Environmental toxicology 39:106-119 PubMed37665165

2023

Total flavonoids of Rhizoma drynariae improves tendon-bone healing for anterior cruciate ligament reconstruction in mice and promotes the osteogenic differentiation of bone mesenchymal stem cells by the ERR1/2-Gga1-TGF-β/MAPK pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Han,Canfeng Wang,Tuo Wang,Yungeng Hu,Hongshun Wang

Experimental and therapeutic medicine 23:229 PubMed35222706

2022

Butorphanol reduces the neuronal inflammatory response and apoptosis via inhibition of p38/JNK/ATF2/p53 signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yingsi Huang,Shuhua Li,Huaxin Chen,Long Feng,Weixiu Yuan,Tao Han

Histology and histopathology 37:269-285 PubMed34908156

2021

Downregulation of KIF15 inhibits the tumorigenesis of non-small-cell lung cancer via inactivating Raf/MEK/ERK signaling.

Applications

Unspecified application

Species

Unspecified reactive species

Yingbin Luo,Bo Zhang,Lili Xu,Minghua Li,Jianchun Wu,Yiyang Zhou,Yan Li

American journal of cancer research 11:4844-4865 PubMed34765296

2021

LncRNA NR2F2-AS1 induces epithelial-mesenchymal transition of non-small cell lung cancer by modulating BVR/ATF-2 pathway via regulating miR-545-5p/c-Met axis.

Applications

Unspecified application

Species

Unspecified reactive species

Cheng Liu,Qun-Gen Li,Yang Zhou,Ying-Yue Cao,Zi-Xin Wei,Ying-Hua Jin,Xin Wang,Ying-Ying Chen,Li Qi,Jian-Xiong Geng,Fang Liu

Frontiers in genetics 12:704712 PubMed34671381

2021

Targeting Long Non-Coding RNA TTN-AS1 Suppresses Bladder Cancer Progression.

Applications

Unspecified application

Species

Unspecified reactive species

Huiyuan Xiao,Wen Huang,Yanlei Li,Rongxin Zhang,Long Yang

American journal of translational research 13:10896-10907 PubMed34650771

2021

LncRNA GAS5 enhances radiosensitivity of hepatocellular carcinoma and restricts tumor growth and metastasis by miR-144-5p/ATF2.

Applications

Unspecified application

Species

Unspecified reactive species

Chuanyun Yu,Yi Liang,Yiqiang Jin,Qinghuan Li

Molecular medicine reports 24: PubMed34498716

2021

Long non‑coding RNA ZSCAN16‑AS1 promotes the malignant properties of hepatocellular carcinoma by decoying microRNA‑451a and consequently increasing ATF2 expression.

Applications

Unspecified application

Species

Unspecified reactive species

Chaohua Lv,Qingsong Wan,Chengxiang Shen,Hongsheng Wu,Bin Zhou,Weiwei Wang
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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