Name: Anti-ATF2 antibody [EPR22938-114]
SKU: ab239361
Rating: 5 (1 reviews)

Rabbit Recombinant Monoclonal ATF2 antibody. Suitable for IP, WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 8 publications.

Images

Western blot - Anti-ATF2 antibody [EPR22938-114] (AB239361), expandable thumbnail

Publications

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

Select an application

Product promiseTestedExpectedPredictedNot recommended

	IP	ChIP	WB	ICC/IF	Flow Cyt (Intra)	IHC-P
Human	Expected	Not recommended	Tested	Not recommended	Expected	Tested
Mouse	Tested	Not recommended	Tested	Not recommended	Tested	Tested
Rat	Expected	Not recommended	Tested	Not recommended	Expected	Tested

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/30	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species	Dilution info	Notes
Species Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Human	Dilution info -	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Mouse	Dilution info -	Notes -
Species Rat	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/1000	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/1000	Notes -
Species Human	Dilution info 1/1000	Notes -

Not recommended
Not recommended

Species	Dilution info	Notes
Species Mouse, Rat, Human	Dilution info -	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/40	Notes -

Expected
Expected

Species	Dilution info	Notes
Species Rat, Human	Dilution info Use at an assay dependent concentration.	Notes -

Tested
Tested

Species	Dilution info	Notes
Species Mouse	Dilution info 1/250	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Rat	Dilution info 1/250	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Species Human	Dilution info 1/250	Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Associated Products

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Target data

See full target information ATF2

Function

Transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro (PubMed:10821277). In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type.

Alternative names

CREB2, CREBP1, ATF2, Cyclic AMP-dependent transcription factor ATF-2, cAMP-dependent transcription factor ATF-2, Activating transcription factor 2, Cyclic AMP-responsive element-binding protein 2, HB16, cAMP response element-binding protein CRE-BP1, CREB-2, cAMP-responsive element-binding protein 2

Recommended products

Rabbit Recombinant Monoclonal ATF2 antibody. Suitable for IP, WB, Flow Cyt (Intra), IHC-P and reacts with Mouse, Rat, Human samples. Cited in 8 publications.

Key facts

Isotype: IgG
Host species: Rabbit
Storage buffer: pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Form: Liquid
Clonality: Monoclonal
Immunogen: The exact immunogen used to generate this antibody is proprietary information.
Clone number: EPR22938-114
Purification technique: Affinity purification Protein A
Concentration

Storage

Shipped at conditions: Blue Ice
Appropriate short-term storage duration: 1-2 weeks
Appropriate short-term storage conditions: +4°C
Appropriate long-term storage conditions: -20°C
Aliquoting information: Upon delivery aliquot
Storage information: Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

The ATF2 protein also referred to as ATF-2 or activating transcription factor 2 plays a significant role as a transcription factor in cellular processes. It weighs approximately 75 kDa and is expressed in many tissues with higher levels in the brain heart and skeletal muscle. Functionally ATF2 belongs to the leucine zipper family of proteins facilitating its ability to bind DNA and regulate the expression of genes involved in stress responses development and growth.

Biological function summary

ATF2 takes part in the regulation of gene expression in response to various stimuli. It often forms a complex with other proteins such as c-Jun when binding to the DNA. This complex then influences the transcription of genes that respond to cellular stress and DNA damage. By phosphorylating specific serine residues cellular kinases activate ATF2 which then translocates to the nucleus where it exerts its function.

Pathways

ATF2 integrates into the MAPK and JNK signaling cascades which are important for transmitting stress signals from the cell surface to the nucleus. Through these pathways ATF2 interacts with proteins such as JNK and p38 MAPK modulating the transcription of downstream genes that control cell proliferation apoptosis and differentiation. Its role in these pathways positions ATF2 as a critical node where various signaling inputs merge to influence cellular outcomes.

Associated diseases and disorders

ATF2 has associations with conditions such as cancer and neurological disorders. Aberrant regulation of ATF2 can contribute to oncogenesis by affecting cell cycle control and apoptosis. For example in melanoma altered ATF2 activity is linked to tumor progression and resistance to apoptosis. Additionally in neurological disorders its interaction with proteins like phospho-c-Jun influences neuronal survival and plasticity implicating ATF2 in pathologies related to neurodegeneration and cognitive dysfunction.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

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10 product images

Western blot - Anti-ATF2 antibody [EPR22938-114] (ab239361)
The molecular weight and degraded fragments observed are consistent with what has been described in the literature (PMID: 9488727, 10207054, 26901653). Suggest use freshly made lysate to minimize protein degradation.
Exposure time: 3 minutes.
Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-ATF2 antibody [EPR22938-114] (ab239361) at 1/1000 dilution
Lane 1: PC-12 (rat adrenal gland pheochromocytoma), whole cell lysate at 20 µg
Lane 2: Rat spleen tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 55 kDa
Observed band size: 70 kDa
Immunoprecipitation - Anti-ATF2 antibody [EPR22938-114] (ab239361)
ATF2 was immunoprecipitated from 0.35 mg NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate with ab239361 at 1/30 dilution (2μg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab239361. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/1000 dilution.
Lane 1: NIH/3T3 (Mouse embryonic fibroblast) whole cell lysate 10μg.
Lane 2: ab239361 IP in NIH/3T3 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab239361 in NIH/3T3 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 8 seconds
Lysate were made freshly and used in IP test immediately to minimize protein degradation. Incubation time was 2h.
The molecular weight and degraded fragments observed are consistent with what has been described in the literature (PMID: 9488727, PMID: 10207054, PMID:26901653).
All lanes: Immunoprecipitation - Anti-ATF2 antibody [EPR22938-114] (ab239361)
Predicted band size: 55 kDa
Western blot - Anti-ATF2 antibody [EPR22938-114] (ab239361)
The molecular weight observed is consistent with what has been described in the literature (PMID:26901653).
Exposure time: 3 minutes.
Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-ATF2 antibody [EPR22938-114] (ab239361) at 1/1000 dilution
All lanes: Mouse brain tissue lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 55 kDa
Observed band size: 70 kDa
Western blot - Anti-ATF2 antibody [EPR22938-114] (ab239361)
The molecular weight observed is consistent with what has been described in the literature (PMID:26901653).
Exposure time: 10 seconds.
Blocking and Diluting Buffer and concentration: 5% NFDM/TBST.
All lanes: Western blot - Anti-ATF2 antibody [EPR22938-114] (ab239361) at 1/1000 dilution
Lane 1: NIH/3T3 (mouse embryonic fibroblast), whole cell lysate at 20 µg
Lane 2: RAW264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage), whole cell lysate at 20 µg
Lane 3: HEK-293T (human embryonic kidney epithelial cell), whole cell lysate at 20 µg
Lane 4: K562 (human chronic myelogenous leukemia lymphoblast), whole cell lysate at 20 µg
Lane 5: Jurkat (human T cell leukemia T lymphocyte), whole cell lysate at 20 µg
Secondary
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Predicted band size: 55 kDa
Observed band size: 70 kDa
Flow Cytometry (Intracellular) - Anti-ATF2 antibody [EPR22938-114] (ab239361)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized RAW 264.7 (Mouse Abelson murine leukemia virus-induced tumor macrophage) cells labeling ATF2 with ab239361 at 1/40 (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF2 antibody [EPR22938-114] (ab239361)
Immunohistochemical analysis of paraffin-embedded rat kidney tissue labeling ATF2 with ab239361 at 1/250 dilution (1.76 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on rat kidney is observed. The section was incubated with ab239361 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF2 antibody [EPR22938-114] (ab239361)
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue labeling ATF2 with ab239361 at 1/250 dilution (1.76 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on mouse kidney is observed. The section was incubated with ab239361 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF2 antibody [EPR22938-114] (ab239361)
Immunohistochemical analysis of paraffin-embedded human kidney carcinoma tissue labeling ATF2 with ab239361 at 1/250 dilution (1.76 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on human kidney carcinoma (PMID: 27377902) is observed. The section was incubated with ab239361 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF2 antibody [EPR22938-114] (ab239361)
Immunohistochemical analysis of paraffin-embedded human kidney tissue labeling ATF2 with ab239361 at 1/250 dilution (1.76 ug/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Nuclear staining on human kidney (PMID: 27377902) is observed. The section was incubated with ab239361 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument. Counterstained with hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Flow Cytometry (Intracellular) - Anti-ATF2 antibody [EPR22938-114] (ab239361)
Intracellular flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized NIH/3T3 (Mouse embryonic fibroblast) cells labeling ATF2 with ab239361 at 1/40 (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor^® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.