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Rabbit Recombinant Monoclonal ATF2 antibody. Carrier free. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

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Images

Immunoprecipitation - Anti-ATF2 antibody [EPR22938-114] - BSA and Azide free (AB256820), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-ATF2 antibody [EPR22938-114] - BSA and Azide free (AB256820), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF2 antibody [EPR22938-114] - BSA and Azide free (AB256820), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF2 antibody [EPR22938-114] - BSA and Azide free (AB256820), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF2 antibody [EPR22938-114] - BSA and Azide free (AB256820), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IHC-PIPChIPWBICC/IFFlow Cyt (Intra)
Human
Tested
Expected
Not recommended
Tested
Not recommended
Expected
Mouse
Tested
Tested
Not recommended
Tested
Not recommended
Tested
Rat
Tested
Expected
Not recommended
Tested
Not recommended
Expected

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Rat
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Mouse
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Mouse, Rat, Human
Dilution info
-
Notes

-

Tested
Tested

Species
Mouse
Dilution info
-
Notes

-

Expected
Expected

Species
Rat, Human
Dilution info
Use at an assay dependent concentration.
Notes

-

Associated Products

Select an associated product type

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Target data

Function

Transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro (PubMed:10821277). In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type.

Alternative names

CREB2, CREBP1, ATF2, Cyclic AMP-dependent transcription factor ATF-2, cAMP-dependent transcription factor ATF-2, Activating transcription factor 2, Cyclic AMP-responsive element-binding protein 2, HB16, cAMP response element-binding protein CRE-BP1, CREB-2, cAMP-responsive element-binding protein 2

Recommended products

Rabbit Recombinant Monoclonal ATF2 antibody. Carrier free. Suitable for IHC-P, IP, WB, Flow Cyt (Intra) and reacts with Mouse, Rat, Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR22938-114
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab256820 is the carrier-free version of Anti-ATF2 antibody [EPR22938-114] ab239361.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

The ATF2 protein also referred to as ATF-2 or activating transcription factor 2 plays a significant role as a transcription factor in cellular processes. It weighs approximately 75 kDa and is expressed in many tissues with higher levels in the brain heart and skeletal muscle. Functionally ATF2 belongs to the leucine zipper family of proteins facilitating its ability to bind DNA and regulate the expression of genes involved in stress responses development and growth.

Biological function summary

ATF2 takes part in the regulation of gene expression in response to various stimuli. It often forms a complex with other proteins such as c-Jun when binding to the DNA. This complex then influences the transcription of genes that respond to cellular stress and DNA damage. By phosphorylating specific serine residues cellular kinases activate ATF2 which then translocates to the nucleus where it exerts its function.

Pathways

ATF2 integrates into the MAPK and JNK signaling cascades which are important for transmitting stress signals from the cell surface to the nucleus. Through these pathways ATF2 interacts with proteins such as JNK and p38 MAPK modulating the transcription of downstream genes that control cell proliferation apoptosis and differentiation. Its role in these pathways positions ATF2 as a critical node where various signaling inputs merge to influence cellular outcomes.

Associated diseases and disorders

ATF2 has associations with conditions such as cancer and neurological disorders. Aberrant regulation of ATF2 can contribute to oncogenesis by affecting cell cycle control and apoptosis. For example in melanoma altered ATF2 activity is linked to tumor progression and resistance to apoptosis. Additionally in neurological disorders its interaction with proteins like phospho-c-Jun influences neuronal survival and plasticity implicating ATF2 in pathologies related to neurodegeneration and cognitive dysfunction.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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