Rabbit Recombinant Monoclonal ATF2 phospho T71 antibody. Carrier free. Suitable for IP, Dot, WB, ICC/IF, ChIC/CUT&RUN-seq and reacts with Human, Synthetic peptide, Mouse samples. Cited in 1 publication.
Images
![Immunoprecipitation - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (AB242381), expandable thumbnail](https://content.abcam.com/products/images/atf2-phospho-t71-antibody-e268-bsa-and-azide-free-ab242381--immunoprecipitation-img9139.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (AB242381)")
![Immunocytochemistry/ Immunofluorescence - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (AB242381), expandable thumbnail](https://content.abcam.com/products/images/atf2-phospho-t71-antibody-e268-bsa-and-azide-free-ab242381--immunocytochemistry-immunofluorescence-img91090.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (AB242381)")
![Dot Blot - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (AB242381), expandable thumbnail](https://content.abcam.com/products/images/atf2-phospho-t71-antibody-e268-bsa-and-azide-free-ab242381--dot-blot-img149842.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (AB242381)")
![ChIC/CUT&RUN sequencing - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (AB242381), expandable thumbnail](https://content.abcam.com/products/images/atf2-phospho-t71-antibody-e268-bsa-and-azide-free-ab242381--chic-cut-run-sequencing-img443785.jpg/_jcr_content/renditions/webp-475.webp "ChIC/CUT&RUN sequencing - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (AB242381)")
![ChIC/CUT&RUN sequencing - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (AB242381), expandable thumbnail](https://content.abcam.com/products/images/atf2-phospho-t71-antibody-e268-bsa-and-azide-free-ab242381--chic-cut-run-sequencing-img443787.jpg/_jcr_content/renditions/webp-475.webp "ChIC/CUT&RUN sequencing - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (AB242381)")
Publications
Filter by
- Immunology 164:754-7652021Transcriptomic landscape of skin lesions in cutaneous leishmaniasis reveals a strong CD8 T cell immunosenescence signature linked to immunopathology.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCarlos Henrique Fantecelle,Luciana Polaco Covre,Renan Garcia de Moura,Herbert Leonel de Matos Guedes,Camila Farias Amorim,Phillip Scott,David Mosser,Aloisio Falqueto,Arne N Akbar,Daniel Claudio Oliveira GomesPubMed 34432883
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | IHC | Flow Cyt | Dot | WB | ICC/IF | ChIC/CUT&RUN-seq | |
|---|---|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Expected | Expected | Tested | Tested |
| Mouse | Predicted | Not recommended | Not recommended | Predicted | Expected | Predicted | Predicted |
| Synthetic peptide | Not recommended | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
IHC
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Synthetic peptide | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ChIC/CUT&RUN-seq
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
12 products for Alternative Product
Target data
Function
Transcriptional activator which regulates the transcription of various genes, including those involved in anti-apoptosis, cell growth, and DNA damage response. Dependent on its binding partner, binds to CRE (cAMP response element) consensus sequences (5'-TGACGTCA-3') or to AP-1 (activator protein 1) consensus sequences (5'-TGACTCA-3'). In the nucleus, contributes to global transcription and the DNA damage response, in addition to specific transcriptional activities that are related to cell development, proliferation and death. In the cytoplasm, interacts with and perturbs HK1- and VDAC1-containing complexes at the mitochondrial outer membrane, thereby impairing mitochondrial membrane potential, inducing mitochondrial leakage and promoting cell death. The phosphorylated form (mediated by ATM) plays a role in the DNA damage response and is involved in the ionizing radiation (IR)-induced S phase checkpoint control and in the recruitment of the MRN complex into the IR-induced foci (IRIF). Exhibits histone acetyltransferase (HAT) activity which specifically acetylates histones H2B and H4 in vitro (PubMed:10821277). In concert with CUL3 and RBX1, promotes the degradation of KAT5 thereby attenuating its ability to acetylate and activate ATM. Can elicit oncogenic or tumor suppressor activities depending on the tissue or cell type.
Alternative names
CREB2, CREBP1, ATF2, Cyclic AMP-dependent transcription factor ATF-2, cAMP-dependent transcription factor ATF-2, Activating transcription factor 2, Cyclic AMP-responsive element-binding protein 2, HB16, cAMP response element-binding protein CRE-BP1, CREB-2, cAMP-responsive element-binding protein 2
Recommended products
Rabbit Recombinant Monoclonal ATF2 phospho T71 antibody. Carrier free. Suitable for IP, Dot, WB, ICC/IF, ChIC/CUT&RUN-seq and reacts with Human, Synthetic peptide, Mouse samples. Cited in 1 publication.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- E268
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab242381 is the carrier-free version of Anti-ATF2 (phospho T71) antibody [E268] ab32019.
SAPK and p38 MAPK activate, in response to cellular stress, ATF2 by phosphorylating the protein at Thr69 and Thr71. Mutations of these sites result in the loss of stress induced transcription by ATF2.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ATF2 protein also referred to as ATF-2 or activating transcription factor 2 plays a significant role as a transcription factor in cellular processes. It weighs approximately 75 kDa and is expressed in many tissues with higher levels in the brain heart and skeletal muscle. Functionally ATF2 belongs to the leucine zipper family of proteins facilitating its ability to bind DNA and regulate the expression of genes involved in stress responses development and growth.
- Biological function summary
ATF2 takes part in the regulation of gene expression in response to various stimuli. It often forms a complex with other proteins such as c-Jun when binding to the DNA. This complex then influences the transcription of genes that respond to cellular stress and DNA damage. By phosphorylating specific serine residues cellular kinases activate ATF2 which then translocates to the nucleus where it exerts its function.
- Pathways
ATF2 integrates into the MAPK and JNK signaling cascades which are important for transmitting stress signals from the cell surface to the nucleus. Through these pathways ATF2 interacts with proteins such as JNK and p38 MAPK modulating the transcription of downstream genes that control cell proliferation apoptosis and differentiation. Its role in these pathways positions ATF2 as a critical node where various signaling inputs merge to influence cellular outcomes.
- Associated diseases and disorders
ATF2 has associations with conditions such as cancer and neurological disorders. Aberrant regulation of ATF2 can contribute to oncogenesis by affecting cell cycle control and apoptosis. For example in melanoma altered ATF2 activity is linked to tumor progression and resistance to apoptosis. Additionally in neurological disorders its interaction with proteins like phospho-c-Jun influences neuronal survival and plasticity implicating ATF2 in pathologies related to neurodegeneration and cognitive dysfunction.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
6 product images
![Immunoprecipitation - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (ab242381), expandable thumbnail](https://content.abcam.com/products/images/atf2-phospho-t71-antibody-e268-bsa-and-azide-free-ab242381--immunoprecipitation-img9139.jpg "Immunoprecipitation - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (ab242381)")
Immunoprecipitation - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (ab242381)
Anti-ATF2 (phospho T71) antibody [E268] ab32019 Immunoprecipitating ATF2 in Human Hela whole cell lysate. For western blotting Anti-ATF2 (phospho T71) antibody [E268] ab32019 (1:1000) was used to confirm successful immunoprecipitation. Blocking and diluting buffer used was 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ATF2 (phospho T71) antibody [E268] ab32019).
All lanes: Immunoprecipitation - Anti-ATF2 (phospho T71) antibody [E268] (Anti-ATF2 (phospho T71) antibody [E268] ab32019) at 0.47 µg/mL
Lane 1: HeLa (human cervix adenocarcinoma) treated with 250ng/ml anisomycin for 30min whole cell lysate (input) at 10 µg
Lane 2: HeLa treated with 250ng/ml anisomycin for 30min whole cell lysate (+)
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-ATF2 (phospho T71) antibody [E268] ab32019 in HeLa treated with 250ng/ml anisomycin for 30min whole cell lysate (-)
SecondaryAll lanes: Immunoprecipitation - VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) at 1/10000 dilution
Predicted band size: 55 kDa
Exposure time: 3min
![Immunocytochemistry/ Immunofluorescence - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (ab242381), expandable thumbnail](https://content.abcam.com/products/images/atf2-phospho-t71-antibody-e268-bsa-and-azide-free-ab242381--immunocytochemistry-immunofluorescence-img91090.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (ab242381)")
Immunocytochemistry/ Immunofluorescence - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (ab242381)
Immunocytochemistry of HeLa (Human epithelial cell line from cervix adenocarcinoma), prepared in FBS free medium overnight labeling ATF2 at 0.9 μg/ml. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% tritonX-100. Alexa Fluor® 488 Goat anti-Rabbit (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) was used as the secondary antibody at 1/500. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889) was used as the counter stain at 2.5 μg/ml. DAPI was used for nuclear counter stain. Confocal image showing the expression was increased on HeLa cells, prepared in FBS free medium overnight, then treated with 250ng/ml anisomycin for 30min.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ATF2 (phospho T71) antibody [E268] ab32019).
![Dot Blot - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (ab242381), expandable thumbnail](https://content.abcam.com/products/images/atf2-phospho-t71-antibody-e268-bsa-and-azide-free-ab242381--dot-blot-img149842.jpg "Dot Blot - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (ab242381)")
Dot Blot - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (ab242381)
Blocking and diluting buffer used was 5% NFDM/TBST.
Primary: Anti-ATF2 (phospho T71) antibody [E268] ab32019, 0.5 ug/ml
Secondary: Goat Anti-Rabbit IgG H&L (HRP) ab97051, 1/100000 dilution![ChIC/CUT&RUN sequencing - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (ab242381), expandable thumbnail](https://content.abcam.com/products/images/atf2-phospho-t71-antibody-e268-bsa-and-azide-free-ab242381--chic-cut-run-sequencing-img443785.jpg "ChIC/CUT&RUN sequencing - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (ab242381)")
ChIC/CUT&RUN sequencing - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (ab242381)
This data was developed using the same antibody clone in a different buffer formulation (Anti-ATF2 (phospho T71) antibody [E268] ab32019).
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 105 K-562 (human chronic myelogenous leukemia lymphoblast) cells treated with anisomycin (25 µg/mL 30 min) and 5 µg of ab Anti-ATF2 (phospho T71) antibody [E268] ab32019[E268]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
![ChIC/CUT&RUN sequencing - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (ab242381), expandable thumbnail](https://content.abcam.com/products/images/atf2-phospho-t71-antibody-e268-bsa-and-azide-free-ab242381--chic-cut-run-sequencing-img443787.jpg "ChIC/CUT&RUN sequencing - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (ab242381)")
ChIC/CUT&RUN sequencing - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (ab242381)
This data was developed using the same antibody clone in a different buffer formulation (Anti-ATF2 (phospho T71) antibody [E268] ab32019).
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 105 K-562 (human chronic myelogenous leukemia lymphoblast) cells treated with anisomycin (25 µg/mL 30 min) and 5 µg of ab Anti-ATF2 (phospho T71) antibody [E268] ab32019[E268]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
![ChIC/CUT&RUN sequencing - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (ab242381), expandable thumbnail](https://content.abcam.com/products/images/atf2-phospho-t71-antibody-e268-bsa-and-azide-free-ab242381--chic-cut-run-sequencing-img443786.jpg "ChIC/CUT&RUN sequencing - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (ab242381)")
ChIC/CUT&RUN sequencing - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (ab242381)
This data was developed using the same antibody clone in a different buffer formulation (Anti-ATF2 (phospho T71) antibody [E268] ab32019).
ChIC/CUT&RUN was performed using a pAG-MNase at a final concentration of 700 ng/mL, 2.5 x 105 K-562 (human chronic myelogenous leukemia lymphoblast) cells treated with anisomycin (25 µg/mL 30 min) and 5 µg of ab Anti-ATF2 (phospho T71) antibody [E268] ab32019[E268]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730 is also shown.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
![ChIC/CUT&RUN sequencing - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (ab242381), expandable thumbnail](https://content.abcam.com/products/images/atf2-phospho-t71-antibody-e268-bsa-and-azide-free-ab242381--chic-cut-run-sequencing-img443786.jpg/_jcr_content/renditions/webp-475.webp "ChIC/CUT&RUN sequencing - Anti-ATF2 (phospho T71) antibody [E268] - BSA and Azide free (ab242381)")
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