JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB233797

Anti-ATF3 antibody [5A9B9]

Be the first to review this product! Submit a review

|

(1 Publication)

Mouse Monoclonal ATF3 antibody. Suitable for Flow Cyt, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human ATF3.

View Alternative Names

Cyclic AMP-dependent transcription factor ATF-3, cAMP-dependent transcription factor ATF-3, Activating transcription factor 3, ATF3

3 Images
Flow Cytometry - Anti-ATF3 antibody [5A9B9] (AB233797)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-ATF3 antibody [5A9B9] (AB233797)

Flow cytometric analysis of A549 (human lung carcinoma cell line) cells labeling ATF3 using ab233797 at 1/200 dilution (green) compared with a negative control (red).

Western blot - Anti-ATF3 antibody [5A9B9] (AB233797)
  • WB

Supplier Data

Western blot - Anti-ATF3 antibody [5A9B9] (AB233797)

Expected MW is 46.5 kDa

All lanes:

Western blot - Anti-ATF3 antibody [5A9B9] (ab233797) at 1/500 dilution

All lanes:

Recombinant human ATF3 protein

Predicted band size: 21 kDa

false

Western blot - Anti-ATF3 antibody [5A9B9] (AB233797)
  • WB

Supplier Data

Western blot - Anti-ATF3 antibody [5A9B9] (AB233797)

All lanes:

Western blot - Anti-ATF3 antibody [5A9B9] (ab233797) at 1/500 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 2:

ATF3 (AA: 1-181)-hIgGFc transfected HEK-293 cell lysate

Predicted band size: 21 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5A9B9

Isotype

IgG1

Carrier free

No

Reacts with

Human

Applications

WB, Flow Cyt

applications

Immunogen

Recombinant Full Length Protein corresponding to Human ATF3.

P18847

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/100 - 1/500", "WB-species-notes": "<p></p>" }, "Mouse": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "FlowCyt-species-checked": "predicted", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Purification notes
Purified from tissue culture supernatant.
Storage buffer
Preservative: 0.05% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATF3 also known as Activating Transcription Factor 3 functions primarily as a stress-responsive transcription factor. This protein has a molecular weight of about 21 kDa and is part of the ATF/CREB family of transcription factors. ATF3 is expressed in various tissues and is known for its role in stress response and regulation of gene expression. The expression levels change in response to cellular stress and it may interact with several other transcription factors to modulate gene expression profiles.
Biological function summary

ATF3 acts as a regulator of cellular stress responses and can influence cell cycle arrest and apoptosis. It does not form a large complex but interacts with diverse partners to execute its functions. Studies often use HCT116 cells to investigate ATF3's role where it can serve as a marker of cellular stress and inflammation. The protein regulates genes involved in maintaining homeostasis in stressed cells.

Pathways

ATF3 is involved in stress response and apoptotic pathways. In the context of apoptosis ATF3 interacts with proteins like p53 and Jun which are important for inducing cell cycle arrest and programmed cell death. In stress response pathways ATF3 modifies transcriptional activity in reaction to various stress signals providing a checkpoint for damaged cells before repairing or entering programmed death.

ATF3 relates to cancer and cardiovascular diseases. In cancer abnormal expression of ATF3 can influence tumor growth and metastasis often acting through its interactions with proteins like NF-kB. Additionally in cardiovascular disorders ATF3's role in regulating stress responses underpins its involvement in conditions linked to inflammation and atherosclerosis again with NF-kB signaling being a relevant pathway of interaction. Researchers continue to study ATF3 to uncover therapeutic targets for these and other diseases.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

This protein binds the cAMP response element (CRE) (consensus : 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter.. Isoform 2. Activates transcription presumably by sequestering inhibitory cofactors away from the promoters.. Isoform 3. Stress-induced isoform, counteracts the transcriptional repression of isoform 1.
See full target information ATF3
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Kidney international 81:458-68 PubMed22166849

2011

Functional metabotropic glutamate receptors 1 and 5 are expressed in murine podocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Leyi Gu,Xinyue Liang,Lihua Wang,Yucheng Yan,Zhaohui Ni,Huili Dai,Jiayuan Gao,Shan Mou,Qin Wang,Xinyu Chen,Liming Wang,Jiaqi Qian
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB191513

Anti-ATF3 [CL1685] antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF3 [CL1685] antibody (AB191513)

  • 0
  • 2
Primary Antibodies

AB207434

Anti-ATF3 antibody [EPR19488] - ChIP Grade

RabMAb|Advanced Validation|Recombinant|KO Validated|20ul selling size

Immunocytochemistry/ Immunofluorescence - Anti-ATF3 antibody [EPR19488] - ChIP Grade (AB207434)

  • 5
  • 1
Primary Antibodies

AB254268

Anti-ATF3 antibody [EPR22610-19] - ChIP Grade

RabMAb|Advanced Validation|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF3 antibody [EPR22610-19] - ChIP Grade (AB254268)

  • 3
  • 2
Primary Antibodies

AB256534

Anti-ATF3 antibody [EPR22610-19] - BSA and Azide free

RabMAb|Advanced Validation|Recombinant|KO Validated

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF3 antibody [EPR22610-19] - BSA and Azide free (AB256534)

  • 0
  • 0
Primary Antibodies

AB232375

Anti-ATF3 antibody [EPR19488] - BSA and Azide free

RabMAb|Advanced Validation|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-ATF3 antibody [EPR19488] - BSA and Azide free (AB232375)

  • 0
  • 0

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB125066

Anti-Glutathione Peroxidase 4 antibody [EPNCIR144]

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Glutathione Peroxidase 4 antibody [EPNCIR144] (AB125066)

  • 5
  • 13
Primary Antibodies

AB68428

Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker

BOND RX™ Validated|20ul selling size|RabMAb|Advanced Validation|Recombinant

Multiplex immunohistochemistry - Anti-GFAP antibody [EPR1034Y] - Astrocyte Marker (AB68428)

  • 5
  • 19

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com