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AB174756

Anti-ATF6 antibody

5

(1 Review)

|

(2 Publications)

Goat Polyclonal ATF6 antibody. Suitable for ICC, WB and reacts with Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ATF6 aa 400-450.

View Alternative Names

Cyclic AMP-dependent transcription factor ATF-6 alpha, cAMP-dependent transcription factor ATF-6 alpha, Activating transcription factor 6 alpha, ATF6-alpha, ATF6

3 Images
Immunocytochemistry - Anti-ATF6 antibody (AB174756)
  • ICC

Supplier Data

Immunocytochemistry - Anti-ATF6 antibody (AB174756)

Immunocytochemistry analysis of paraformaldehyde fixed HeLa cells labelling ATF6 with ab174756 at 10 μg/mL. Cells permeabilized with 0.15% Triton. Primary incubation for 1 hour followed by Alexa Fluor® 488 secondary antibody (2 μg/mL). The nuclear stain is DAPI (blue).

Negative control : Unimmunized goat IgG (10 μg/mL) followed by Alexa Fluor® 488 secondary antibody (2 μg/mL).

Immunocytochemistry - Anti-ATF6 antibody (AB174756)
  • ICC

Supplier Data

Immunocytochemistry - Anti-ATF6 antibody (AB174756)

Immunocytochemistry analysis of paraformaldehyde fixed A431 cells labelling ATF6 with ab174756 at 10 μg/mL. Cells permeabilized with 0.15% Triton. Primary incubation for 1 hour followed by Alexa Fluor® 488 secondary antibody (2 μg/mL). The nuclear stain is DAPI (blue).

Negative control : Unimmunized goat IgG (10 μg/mL) followed by Alexa Fluor® 488 secondary antibody (2 μg/mL).

Western blot - Anti-ATF6 antibody (AB174756)
  • WB

Supplier Data

Western blot - Anti-ATF6 antibody (AB174756)

All lanes:

Western blot - Anti-ATF6 antibody (ab174756) at 1 µg/mL

Lane 1:

HeLa whole cell lysate in RIPA buffer at 35 µg

Lane 2:

MCF7 whole cell lysate in RIPA buffer at 35 µg

Lane 3:

HeLa nuclear cell lysate in RIPA buffer at 35 µg

Lane 4:

Jurkat nuclear cell lysate in RIPA buffer at 35 µg

Predicted band size: 74 kDa

Observed band size: 75-80 kDa

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Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC, WB

applications

Immunogen

Synthetic Peptide within Human ATF6 aa 400-450. The exact immunogen used to generate this antibody is proprietary information.

P18850

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICC" : {"fullname" : "Immunocytochemistry", "shortname":"ICC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICC-species-checked": "testedAndGuaranteed", "ICC-species-dilution-info": "10 µg/mL", "ICC-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.3-2 µg/mL", "WB-species-notes": "<p>A 1 hour primary incubation is recommended for this product.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Activating Transcription Factor 6 (ATF6) also known as ATF 6 is a significant protein in the endoplasmic reticulum (ER) stress response. The ATF6 protein has a molecular weight of approximately 84 kDa. It expresses predominantly in the endoplasmic reticulum membrane and plays a critical role in the unfolded protein response (UPR). During ER stress ATF6 relocates to the Golgi apparatus where it undergoes cleavage to become an active transcription factor. This process helps the cell to manage the accumulation of unfolded proteins.
Biological function summary

ATF6 operates as part of the transcription regulation mechanisms responding to ER stress. The ATF6 protein is essential in managing the expression of chaperone genes and ER-associated degradation (ERAD) components thereby maintaining protein homeostasis. ATF6 itself does not operate within a traditional complex but its activation involves proteolytic cleavage which subsequently releases the active form to the nucleus where it influences gene expression to alleviate stress conditions.

Pathways

ATF6 is prominently involved in the unfolded protein response pathway which manages cell survival and stress adaptation. This pathway closely interacts with other proteins like IRE1 and PERK forming a network that modulates the transcription of UPR target genes. Additionally ATF6 contributes to checkpoint control pathways that stabilize cellular environment by regulating genes related to chaperone and protein folding.

Malfunction or dysregulation of ATF6 links to conditions like diabetes and neurodegenerative diseases. In diabetes improper ATF6 function affects insulin signaling and glucose homeostasis often alongside proteins such as XBP1. In neurodegenerative diseases an inappropriate response to ER stress influences the progression of disorders like Alzheimer's disease where protein misfolding and aggregation play pivotal roles. Understanding how ATF6 interplays with these diseases could lead to new therapeutic avenues.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cyclic AMP-dependent transcription factor ATF-6 alpha. Precursor of the transcription factor form (Processed cyclic AMP-dependent transcription factor ATF-6 alpha), which is embedded in the endoplasmic reticulum membrane (PubMed : 10564271, PubMed : 11158310, PubMed : 11779464). Endoplasmic reticulum stress promotes processing of this form, releasing the transcription factor form that translocates into the nucleus, where it activates transcription of genes involved in the unfolded protein response (UPR) (PubMed : 10564271, PubMed : 11158310, PubMed : 11779464).. Processed cyclic AMP-dependent transcription factor ATF-6 alpha. Transcription factor that initiates the unfolded protein response (UPR) during endoplasmic reticulum stress by activating transcription of genes involved in the UPR (PubMed : 10564271, PubMed : 11158310, PubMed : 11163209, PubMed : 11779464). Binds DNA on the 5'-CCAC[GA]-3'half of the ER stress response element (ERSE) (5'-CCAAT-N(9)-CCAC[GA]-3') and of ERSE II (5'-ATTGG-N-CCACG-3') (PubMed : 10564271, PubMed : 11158310, PubMed : 11779464). Binding to ERSE requires binding of NF-Y to ERSE. Could also be involved in activation of transcription by the serum response factor (PubMed : 10564271, PubMed : 11158310, PubMed : 11779464). May play a role in foveal development and cone function in the retina (PubMed : 26029869).
See full target information ATF6
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

American journal of translational research 12:4354-4370 PubMed32913511

2020

Rho-kinase inhibition by Fasudil promotes tumor maturation and apoptosis in small-cell lung cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Zitian Huo,Yinxia Su,Yuting Dong,Yiyun Zheng,Qian Zhang,Yaqi Duan,Guoping Wang

Cancer medicine 8:6344-6357 PubMed31464090

2019

RIG-I promotes IFN/JAK2 expression and the endoplasmic reticulum stress response to inhibit chemoradiation resistance in nasopharyngeal carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Di Jing,Weibing Zhou,Lin Shen,Qian Zhang,Wang-Ti Xie,Erdong Shen,Zhi Li,Liang-Fang Shen,Lun-Quan Sun
View all publications
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Product promise

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