Anti-ATF6 antibody

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATF6 antibody (AB203119)

Immunohistochemical analysis of HeLa cells stained for ATF6 using ab203119 at a dilution of 1 : 100.

• Flow Cyt (Intra)

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Flow Cytometry (Intracellular) - Anti-ATF6 antibody (AB203119)

Jurkat cells were fixed with 4% PFA for 10min at room temperature,permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. Cells were then stained with ATF6 Antibody (ab203119) at 1 : 100 dilution in blocking buffer and incubated for 30 min at room temperature, washed twice with 2%BSA in PBS, followed by secondary antibody incubation for 40 min at room temperature. Acquisitions of 20,000 events were performed. Cells stained with primary antibody (green), and isotype control (orange).

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-ATF6 antibody (AB203119)

Immunocytochemistry/ Immunofluorescence analysis of U-251 cells labeling ATF6 with ab203119 at 1/200 (diluted in PBS and incubated for 2 hours at 37°C). Goat Anti-Rabbit IgG (H+L) FITC conjugated antibody was used as the secondary antibody. Nuclei were stained with DAPI (blue).

• WB

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Western blot - Anti-ATF6 antibody (AB203119)

All lanes:

Western blot - Anti-ATF6 antibody (ab203119) at 1/1000 dilution

Lane 1:

HUVEC cell lysate

Lane 2:

Raji cell lysate

Lane 3:

Jurkat cell lysate

Lane 4:

A549 cell lysate

Lane 5:

thp-1 cell lysate

Lane 6:

293T cell lysate

Lane 7:

du145 cell lysate

Secondary

All lanes:

Conjugated secondary at 1/2000 dilution

Predicted band size: 74 kDa

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