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AB87844

Anti-ATF7 antibody

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(1 Publication)

Rabbit Polyclonal ATF7 antibody. Suitable for IP, WB and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ATF7 aa 400 to C-terminus.

View Alternative Names

ATFA, ATF7, Cyclic AMP-dependent transcription factor ATF-7, cAMP-dependent transcription factor ATF-7, Activating transcription factor 7, Transcription factor ATF-A

2 Images
Immunoprecipitation - Anti-ATF7 antibody (AB87844)
  • IP

Unknown

Immunoprecipitation - Anti-ATF7 antibody (AB87844)

Detection of human ATF7 by Immunoprecipitation from HeLa whole cell lysate (1 mg for IP, 20% of IP loaded), using ab87844 at 10 µg/mg lysate for IP. Subsequent Western blot detection used ab87844 at 1 µg/ml.
Developed using the ECL technique, with an exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-ATF7 antibody (ab87844)

Predicted band size: 53 kDa

false

Western blot - Anti-ATF7 antibody (AB87844)
  • WB

Unknown

Western blot - Anti-ATF7 antibody (AB87844)

All lanes:

Western blot - Anti-ATF7 antibody (ab87844) at 0.1 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 5 µg

Predicted band size: 53 kDa

Observed band size: 63 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human ATF7 aa 400 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

P17544

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "10-15 µg/mg of lysate", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Elephant": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Purification notes
Affinity purified using an epitope specific to ATF7 immobilized on solid support.
Storage buffer
pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: Tris citrate/phosphate
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATF7 (activating transcription factor 7) is a transcription factor that belongs to the ATF/CREB family. It has alternative names such as ATFa and B-ATF. Mechanically ATF7 regulates transcription by binding to cyclic AMP response elements (CRE) in DNA. This protein has a mass of approximately 55 kDa. ATF7 is expressed in various tissues throughout the body including the brain liver and spleen supporting its involvement in multiple physiological processes.
Biological function summary

ATF7 functions in cellular stress responses and cell differentiation. It often forms a complex with other transcription factors to exert its effects on gene expression. ATF7 is known to influence the expression of genes involved in stress response pathways and apoptosis. By regulating these genes ATF7 plays a role in how cells react to environmental changes and maintain homeostasis.

Pathways

ATF7 is involved in significant signaling pathways such as the MAPK and JNK pathways. These pathways are important for transmitting signals from the cell surface to the nucleus affecting gene expression. ATF7 interacts with proteins such as c-Jun and ATF2 within these pathways facilitating cellular responses to stress and growth signals.

ATF7 has been linked to certain cancers and neurodegenerative diseases. Abnormal expression or mutation of ATF7 can contribute to the progression of cancer possibly through its interaction with proteins like c-Myc. In neurodegenerative conditions ATF7 might play a role in neuronal cell death potentially in connection with proteins involved in stress signaling such as p53. Understanding the role of ATF7 in these disorders may provide insight into new therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Stress-responsive chromatin regulator that plays a role in various biological processes including innate immunological memory, adipocyte differentiation or telomerase regulation (PubMed : 29490055). In absence of stress, contributes to the formation of heterochromatin and heterochromatin-like structure by recruiting histone H3K9 tri- and di-methyltransferases thus silencing the transcription of target genes such as STAT1 in adipocytes, or genes involved in innate immunity in macrophages and adipocytes (By similarity). Stress induces ATF7 phosphorylation that disrupts interactions with histone methyltransferase and enhances the association with coactivators containing histone acetyltransferase and/or histone demethylase, leading to disruption of the heterochromatin-like structure and subsequently transcriptional activation (By similarity). In response to TNF, which is induced by various stresses, phosphorylated ATF7 and telomerase are released from telomeres leading to telomere shortening (PubMed : 29490055). Also plays a role in maintaining epithelial regenerative capacity and protecting against cell death during intestinal epithelial damage and repair (By similarity).. Isoform 4. Acts as a dominant repressor of the E-selectin/NF-ELAM1/delta-A promoter.. Isoform 5. Acts as a negative regulator, inhibiting both ATF2 and ATF7 transcriptional activities. It may exert these effects by sequestrating in the cytoplasm the Thr-53 phosphorylating kinase, preventing activation.
See full target information ATF7
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell transplantation 31:9636897221116085 PubMed36062473

2022

Establishment of a Zebrafish Xenograft Model for Investigation of Nasopharyngeal Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Enyu Huang,Haofeng Huang,Longji Wu,Binbin Li,Zhiwei He,Jingjing Zhang
View all publications
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Product promise

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