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AB247975

Anti-ATG10 antibody [EPR4804] - BSA and Azide free

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Rabbit Recombinant Monoclonal ATG10 antibody. Carrier free. Suitable for WB and reacts with Human samples.

View Alternative Names

APG10L, PP12616, ATG10, Ubiquitin-like-conjugating enzyme ATG10, Autophagy-related protein 10, APG10-like

3 Images
Western blot - Anti-ATG10 antibody [EPR4804] - BSA and Azide free (AB247975)
  • WB

Lab

Western blot - Anti-ATG10 antibody [EPR4804] - BSA and Azide free (AB247975)

This data was developed using ab124711, the same antibody clone in a different buffer formulation.

Lanes 1-4 : Merged signal (red and green). Green - ab124711 observed at 28 kDa. Red - loading control ab8245 observed at 36 kDa.

ab124711 Anti-ATG10 antibody [EPR4804] was shown to specifically react with ATG10 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab265682 (knockout cell lysate ab258786) was used. Wild-type and ATG10 knockout samples were subjected to SDS-PAGE. ab124711 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ATG10 antibody [EPR4804] (<a href='/en-us/products/primary-antibodies/atg10-antibody-epr4804-ab124711'>ab124711</a>) at 1/1000 dilution

Lane 1:

Wild-type HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

ATG10 knockout HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 20 µg

Lane 2:

Western blot - Human ATG10 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-atg10-knockout-hela-cell-line-ab265682'>ab265682</a>)

Lane 3:

K562 (Human chronic myelogenous leukemia lymphoblast cell line) whole cell lysate at 20 µg

Lane 4:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 25 kDa

Observed band size: 28 kDa

false

Western blot - Anti-ATG10 antibody [EPR4804] - BSA and Azide free (AB247975)
  • WB

Unknown

Western blot - Anti-ATG10 antibody [EPR4804] - BSA and Azide free (AB247975)

This data was developed using ab124711, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ATG10 antibody [EPR4804] (<a href='/en-us/products/primary-antibodies/atg10-antibody-epr4804-ab124711'>ab124711</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysates at 10 µg

Lane 2:

K562 cell lysates at 10 µg

Lane 3:

293T cell lysates at 10 µg

Predicted band size: 25 kDa

false

OI-RD Scanning - Anti-ATG10 antibody [EPR4804] - BSA and Azide free (AB247975)
  • OI-RD Scanning

Unknown

OI-RD Scanning - Anti-ATG10 antibody [EPR4804] - BSA and Azide free (AB247975)

We have systematically measured KD (the equilibrium dissociation constant between the antibody and its antigen), of more than 840 recombinant antibodies to assess not only their individual KD values but also to see the average affinity of antibody. Based on the comparison with published literature values for mouse monoclonal antibodies, Recombinant antibodies appear to be on average 1-2 order of magnitude higher affinity.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR4804

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab247975 is the carrier-free version of ab124711.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATG10 also known as Autophagy-Related Protein 10 is an important player in the autophagy pathway. This protein weighs approximately 21 kDa and is expressed in a variety of tissues with high levels observed in the heart and skeletal muscle. Mechanically ATG10 acts as an E2-like enzyme important for the conjugation of ATG12 to ATG5 forming the ATG12–ATG5 conjugate essential for autophagosome formation.
Biological function summary

The autophagy process involves ATG10 in the covalent attachment of ATG12 to ATG5 a step critical for elongation and completion of autophagosomes. This protein functions as part of the ATG12–ATG5–ATG16L1 complex which plays a structural role in the phagophore membrane. Without ATG10's activity efficient autophagosome assembly cannot occur highlighting its importance in maintaining cellular homeostasis through regulated turnover of cellular components.

Pathways

ATG10 significantly impacts the macroautophagy pathway and links to metabolic control pathways. It interacts closely with proteins such as ATG5 and ATG7 which are necessary for initiating and executing the autophagy process. This involvement ensures the proper degradation of damaged organelles and recycling of nutrients during cellular stress.

Researchers have connected ATG10 activity to conditions like neurodegenerative diseases and cancers. Dysregulation of ATG10 can impair autophagic function which may contribute to Alzheimer's disease progression by preventing the clearance of protein aggregates. Additionally alteration in ATG10 expression has associations with certain cancers where it impacts cancer cell survival and proliferation. Understanding ATG10's interactions with other proteins like BECN1 could further elucidate its role in these diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E2-like enzyme involved in autophagy. Acts as an E2-like enzyme that catalyzes the conjugation of ATG12 to ATG5. ATG12 conjugation to ATG5 is required for autophagy. Likely serves as an ATG5-recognition molecule. Not involved in ATG12 conjugation to ATG3 (By similarity). Plays a role in adenovirus-mediated cell lysis.
See full target information ATG10
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Complementary Products

Primary Antibodies

AB124711

Anti-ATG10 antibody [EPR4804]

RabMAb|Recombinant|KO Validated

Western blot - Anti-ATG10 antibody [EPR4804] (AB124711)

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Cell Lines & Lysates

AB265682

Human ATG10 knockout HeLa cell line

Advanced Validation

Western blot - Human ATG10 knockout HeLa cell line (AB265682)

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com