Rabbit Polyclonal ATG12 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 31 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ATG12 aa 1 to C-terminus.
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- Genes & diseases 10:1582-15952023Progranulin regulation of autophagy contributes to its chondroprotective effect in osteoarthritis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYiming Pan,Yuyou Yang,Mengtian Fan,Cheng Chen,Rong Jiang,Li Liang,Menglin Xian,Biao Kuang,Nana Geng,Naibo Feng,Lin Deng,Wei Zheng,Fengmei Zhang,Xiaoli Li,Fengjin GuoPubMed 37397563
- Cell death discovery 9:152023Neuroprotective effect of astragalin via activating PI3K/Akt-mTOR-mediated autophagy on APP/PS1 mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCui-Zhu Yang,Shu-Han Wang,Run-Heng Zhang,Jia-Hong Lin,Ying-Hong Tian,Ya-Qi Yang,Jing Liu,Yu-Xin MaPubMed 36681681
- Cell reports 41:1114352022Destabilization of TP53 by USP10 is essential for neonatal autophagy and survival.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHongchang Li,Chaonan Li,Wenjing Zhai,Xin Zhang,Lei Li,Bo Wu,Biyue Yu,Pengfei Zhang,Jie Li,Chun-Ping Cui,Lingqiang ZhangPubMed 36198274
- Journal of virology 96:e01375222022Peste des Petits Ruminants Virus Upregulates STING to Activate ATF6-Mediated Autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRui Zhang,Hanrui Lin,Qing You,Zhixiong Zhang,Ling Bai,Fei Chen,Xueliang Zhu,Zhidong Zhang,Yanmin LiPubMed 36197111
- Frontiers in oncology 12:9298382022ACO1 and IREB2 downregulation confer poor prognosis and correlate with autophagy-related ferroptosis and immune infiltration in KIRC.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTing Zhu,Zhuoyu Xiao,Haoyu Yuan,Hu Tian,Taoyi Chen,Qi Chen,Mingkun Chen,Jiankun Yang,Qizhao Zhou,Wenbin Guo,Kangyi Xue,Ming Xia,Jiming Bao,Cheng Yang,Haifeng Duan,Hongyi Wang,Zhipeng Huang,Cundong Liu,Junhao ZhouPubMed 36059676
- Journal of inflammation research 15:2333-23472022Identification of Key Genes and Potential Mechanisms Based on the Autophagy Regulatory Network in Osteoclasts Using a Murine Osteoarthritis Model.Applications:Unspecified applicationReactive species:Unspecified reactive speciesHaifeng Zhang,Houyi Sun,Wei Zhang,Yaozeng Xu,Dechun GengPubMed 35437349
- Cell & bioscience 11:1702021Huaier polysaccharides suppress triple-negative breast cancer metastasis and epithelial-mesenchymal transition by inducing autophagic degradation of Snail.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYuan Tian,Jin Wu,Lingjuan Zeng,Linxi Zhou,Ying Hu,Qinwen Pan,Wei Liu,Yuzhao Yan,Ziwei Wu,Zhaoyu Wang,Zhen Zeng,Peng Tang,Jun Jiang,Minghao WangPubMed 34481526
- Scientific reports 11:148982021Interleukin-27 promotes autophagy in human serum-induced primary macrophages via an mTOR- and LC3-independent pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSylvain Laverdure,Ziqiu Wang,Jun Yang,Takuya Yamamoto,Tima Thomas,Toyotaka Sato,Kunio Nagashima,Tomozumi ImamichiPubMed 34290273
- Frontiers in cell and developmental biology 9:6219062021Sp1 Targeted PARP1 Inhibition Protects Cardiomyocytes From Myocardial Ischemia-Reperfusion Injury via Downregulation of Autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYifeng Xu,Boqian Wang,Xiaoxiao Liu,Yunfei Deng,Yanqi Zhu,Feng Zhu,Yanyan Liang,Hongli LiPubMed 34124031
- Cell biology international 45:305-3192020PSPH induces cell autophagy and promotes cell proliferation and invasion in the hepatocellular carcinoma cell line Huh7 via the AMPK/mTOR/ULK1 signaling pathway.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJianli Zhang,Erhao Wang,Lei Zhang,Bo ZhouPubMed 33079432
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human ATG12 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O94817
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Reactivity data
Select an application| WB | IHC-P | ICC/IF | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Tested | Expected | Expected |
| Rat | Tested | Expected | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/500 - 1/3000 | Notes - |
Species Rat | Dilution info 1/500 - 1/3000 | Notes - |
Species Human | Dilution info 1/500 - 1/3000 | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 - 1/1000 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Associated Products
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Target data
Function
Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. As part of the ATG8 conjugation system with ATG5 and ATG16L1, required for recruitment of LRRK2 to stressed lysosomes and induction of LRRK2 kinase activity in response to lysosomal stress (By similarity). (Microbial infection) May act as a proviral factor. In association with ATG5, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection (PubMed:17709747). Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for the initiation of HCV replication, but not required once infection is established (PubMed:19666601).
Alternative names
APG12, APG12L, ATG12, Ubiquitin-like protein ATG12, Autophagy-related protein 12, APG12-like
Recommended products
Rabbit Polyclonal ATG12 antibody. Suitable for WB, IHC-P, ICC/IF and reacts with Mouse, Rat, Human samples. Cited in 31 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ATG12 aa 1 to C-terminus.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human ATG12 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O94817
- Purification technique
- Affinity purification Immunogen
- Specificity
ab155589 detects free ATG12 and ATG12 conjugated to Atg5.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ATG12 also known as Autophagy-related protein 12 plays a critical role in autophagy an essential cellular process for degrading and recycling cellular components. The molecular weight of ATG12 is around 14 kDa. It is part of the ATG12-conjugation system and is invariably expressed in various tissues with notable presence in the liver brain and heart. ATG12 forms a covalent bond with ATG5 in a ubiquitin-like conjugation pathway which is significant for this mechanism.
- Biological function summary
ATG12 is vital for the formation of autophagosomes which are double-membraned vesicles that sequester cellular components for degradation. It functions as part of a complex by binding to ATG5 and further associates with ATG16L1 to form a larger ATG12-ATG5-ATG16 complex. This complex localizes to the outer membrane of the growing autophagosome facilitating elongation and closure of the vesicle making it effective in the regulation of autophagy.
- Pathways
ATG12's interaction with other proteins like ATG5 places it in the macroautophagy pathway an important process maintaining cellular homeostasis and turnover of cellular components. In addition to this ATG12 also contributes to cellular responses to stress conditions via this pathway. Furthermore it has connections with signaling pathways involving proteins such as LC3 which marks autophagosomal membranes and interacts with ATG12-conjugated complexes enhancing the autophagic flux.
- Associated diseases and disorders
Researchers associate dysregulated expression of ATG12 with neurodegenerative diseases such as Parkinson’s disease where impaired autophagy leads to toxic protein accumulation. Additionally alterations in ATG12 expression have links to cancer as enhanced autophagy in some tumor cells supports survival under metabolic stress. Both disorders demonstrate connections to proteins involved in the autophagy pathway suggesting therapeutic potential in modulating ATG12 activity.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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6 product images
Western blot - Anti-ATG12 antibody (ab155589)
False colour image of Western blot: Anti-ATG12 antibody staining at 1/500 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab155589 was shown to bind specifically to ATG12. A band likely to be the unfunctional complex with ATG5 was observed at 52 kDa in wild-type THP-1 cell lysates with no signal observed at this size in Atg12 knockout cell line Human ATG12 knockout THP-1 cell line ab277831 (knockout cell lysate ab278183) - unconjugated functional form not observed at 15 kD. To generate this image, wild-type and Atg12 knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3% milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-ATG12 antibody (ab155589) at 1/500 dilution
Lane 1: Wild-type THP-1 cell lysate at 20 µg
Lane 2: ATG12 knockout THP-1 cell lysate at 20 µg
Lane 2: Western blot - Human ATG12 knockout THP-1 cell line (Human ATG12 knockout THP-1 cell line ab277831)
Lane 3: HCT 116 cell lysate at 20 µg
Lane 4: HeLa cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 15 kDa
Observed band size: 52 kDa
Western blot - Anti-ATG12 antibody (ab155589)
12% SDS PAGE
All lanes: Western blot - Anti-ATG12 antibody (ab155589) at 1/1000 dilution
All lanes: NIH3T3 whole cell lysate at 30 µg
Predicted band size: 15 kDa
Western blot - Anti-ATG12 antibody (ab155589)
12% SDS PAGE
All lanes: Western blot - Anti-ATG12 antibody (ab155589) at 1/1000 dilution
All lanes: Rat brain whole cell lysate at 50 µg
Predicted band size: 15 kDa
Western blot - Anti-ATG12 antibody (ab155589)
12% SDS PAGE
All lanes: Western blot - Anti-ATG12 antibody (ab155589) at 1/1000 dilution
Lane 1: 293T whole cell lysate at 30 µg
Lane 2: A431 whole cell lysate at 30 µg
Predicted band size: 15 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG12 antibody (ab155589)
Immunohistochemical analysis of paraffin-embedded U87 (human) xenograft tissue labeling ATG12 with ab155589 at 1/500 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-ATG12 antibody (ab155589)
Immunofluorescent analysis of paraformaldehyde-fixed HeLa cells labeling ATG12 with ab155589 at 1/500 dilution. Lower panels co-stained with Hoechst 33342.
Cells were fixed in 4% paraformaldehyde for 15 minutes.
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