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AB228494

Anti-ATG12 antibody - N-terminal

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(1 Publication)

Rabbit Polyclonal ATG12 antibody. N-terminal. Suitable for Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Human ATG12.

View Alternative Names

APG12, APG12L, ATG12, Ubiquitin-like protein ATG12, Autophagy-related protein 12, APG12-like

1 Images
Flow Cytometry (Intracellular) - Anti-ATG12 antibody - N-terminal (AB228494)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ATG12 antibody - N-terminal (AB228494)

Flow cytometric analysis of methanol-fixed, 0.1% Tween-20-permeabilized SH-SY5Y (human neuroblastoma cell line from bone marrow) cell line labeling ATG12 with ab228494 at 2 μg per 106 cells (black) compared with a Rabbit IgG Control (white). Donkey anti-Rabbit PE at 1/100 dilution was used as the secondary antibody.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt (Intra)

applications

Immunogen

Synthetic Peptide within Human ATG12. The exact immunogen used to generate this antibody is proprietary information.

O94817

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "2 µg/cells for 6 µg/cells", "FlowCytIntra-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Lyophilized
Reconstitution
reconstitute with water at 500µL
Purification technique
Affinity purification Protein G
Storage buffer
pH: 7 - 7.4
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATG12 also known as Autophagy-related protein 12 plays a critical role in autophagy an essential cellular process for degrading and recycling cellular components. The molecular weight of ATG12 is around 14 kDa. It is part of the ATG12-conjugation system and is invariably expressed in various tissues with notable presence in the liver brain and heart. ATG12 forms a covalent bond with ATG5 in a ubiquitin-like conjugation pathway which is significant for this mechanism.
Biological function summary

ATG12 is vital for the formation of autophagosomes which are double-membraned vesicles that sequester cellular components for degradation. It functions as part of a complex by binding to ATG5 and further associates with ATG16L1 to form a larger ATG12-ATG5-ATG16 complex. This complex localizes to the outer membrane of the growing autophagosome facilitating elongation and closure of the vesicle making it effective in the regulation of autophagy.

Pathways

ATG12's interaction with other proteins like ATG5 places it in the macroautophagy pathway an important process maintaining cellular homeostasis and turnover of cellular components. In addition to this ATG12 also contributes to cellular responses to stress conditions via this pathway. Furthermore it has connections with signaling pathways involving proteins such as LC3 which marks autophagosomal membranes and interacts with ATG12-conjugated complexes enhancing the autophagic flux.

Researchers associate dysregulated expression of ATG12 with neurodegenerative diseases such as Parkinson’s disease where impaired autophagy leads to toxic protein accumulation. Additionally alterations in ATG12 expression have links to cancer as enhanced autophagy in some tumor cells supports survival under metabolic stress. Both disorders demonstrate connections to proteins involved in the autophagy pathway suggesting therapeutic potential in modulating ATG12 activity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. As part of the ATG8 conjugation system with ATG5 and ATG16L1, required for recruitment of LRRK2 to stressed lysosomes and induction of LRRK2 kinase activity in response to lysosomal stress (By similarity).. (Microbial infection) May act as a proviral factor. In association with ATG5, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection (PubMed : 17709747). Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for the initiation of HCV replication, but not required once infection is established (PubMed : 19666601).
See full target information ATG12
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

CNS neuroscience & therapeutics 31:e70578 PubMed40904192

2025

Endothelin-Converting Enzyme-Like 1 Regulated by LIF Contributes to Chronic Constriction Injury-Induced Neuropathic Pain in Mice.

Applications

Unspecified application

Species

Unspecified reactive species

Feng Gao,Yuchen Pan,Yong Huang,Chen Gu,Xiaowei Song,Cunjin Wang
View all publications
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