Rabbit Recombinant Multiclonal ATG12 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human ATG12.
Images
![Western blot - Anti-ATG12 antibody [RP23040104] (AB313434), expandable thumbnail](https://content.abcam.com/products/images/atg12-antibody-rp23040104-ab313434--western-blot-img417370.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ATG12 antibody [RP23040104] (AB313434)")
![Western blot - Anti-ATG12 antibody [RP23040104] (AB313434), expandable thumbnail](https://content.abcam.com/products/images/atg12-antibody-rp23040104-ab313434--western-blot-img417365.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ATG12 antibody [RP23040104] (AB313434)")
![Immunocytochemistry/ Immunofluorescence - Anti-ATG12 antibody [RP23040104] (AB313434), expandable thumbnail](https://content.abcam.com/products/images/atg12-antibody-rp23040104-ab313434--immunocytochemistry-immunofluorescence-img417438.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-ATG12 antibody [RP23040104] (AB313434)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS- Form
- Liquid
- Clonality
- Multiclonal
Immunogens
This product was produced with the following immunogens:
Reactivity data
Select an application| WB | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
| Mouse | Tested | Expected |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.50000-4.00000 µg/mL | Notes - |
Species Mouse | Dilution info 2.50000-4.00000 µg/mL | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2 µg/mL | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. As part of the ATG8 conjugation system with ATG5 and ATG16L1, required for recruitment of LRRK2 to stressed lysosomes and induction of LRRK2 kinase activity in response to lysosomal stress (By similarity). (Microbial infection) May act as a proviral factor. In association with ATG5, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection (PubMed:17709747). Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for the initiation of HCV replication, but not required once infection is established (PubMed:19666601).
Alternative names
APG12, APG12L, ATG12, Ubiquitin-like protein ATG12, Autophagy-related protein 12, APG12-like
Recommended products
Rabbit Recombinant Multiclonal ATG12 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human ATG12.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.09% Sodium azide
Constituents: 99.91% PBS- Form
- Liquid
- Clonality
- Multiclonal
- Immunogens
This product was produced with the following immunogens:
- Clone number
- RP23040104
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains.
Recombinant multiclonal antibodies offer the sensitivity of polyclonal antibodies by recognising multiple epitopes, along with consistency of a recombinant antibody.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-ATG12 antibody [RP23040104] (ab313434), expandable thumbnail](https://content.abcam.com/products/images/atg12-antibody-rp23040104-ab313434--western-blot-img417370.jpg "Western blot - Anti-ATG12 antibody [RP23040104] (ab313434)")
Western blot - Anti-ATG12 antibody [RP23040104] (ab313434)
All lanes: Western blot - Anti-ATG12 antibody [RP23040104] (ab313434) at 4 µg/mL
Lane 1: HEK293 whole cell lysate at 30 µg
Lane 2: THP-1 whole cell lysate at 30 µg
Lane 3: PANC-1 whole cell lysate at 30 µg
Lane 4: HCT-116 whole cell lysate at 30 µg
Lane 5: C2C12 whole cell lysate at 30 µg
Lane 6: NIH 3T3 whole cell lysate at 30 µg
Lane 7: Mouse colon tissue lysate at 30 µg
Lane 8: Mouse Kidney tissue lysate at 30 µg
SecondaryAll lanes: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution
Developed using the ECL technique.
Predicted band size: 15.113 kDa
Observed band size: 55 kDa
![Western blot - Anti-ATG12 antibody [RP23040104] (ab313434), expandable thumbnail](https://content.abcam.com/products/images/atg12-antibody-rp23040104-ab313434--western-blot-img417365.jpg "Western blot - Anti-ATG12 antibody [RP23040104] (ab313434)")
Western blot - Anti-ATG12 antibody [RP23040104] (ab313434)
All lanes: Western blot - Anti-ATG12 antibody [RP23040104] (ab313434) at 1/350 dilution
Lane 1: Untransfected U-2OS cell lysate
Lane 2: non-targeting scrambled siRNA transfected U-2OS cell lysate
Lane 3: ATG12 knockdown u-2OS cell lysate
SecondaryAll lanes: Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution
Predicted band size: 15.113 kDa
Observed band size: 55 kDa
![Immunocytochemistry/ Immunofluorescence - Anti-ATG12 antibody [RP23040104] (ab313434), expandable thumbnail](https://content.abcam.com/products/images/atg12-antibody-rp23040104-ab313434--immunocytochemistry-immunofluorescence-img417438.jpg "Immunocytochemistry/ Immunofluorescence - Anti-ATG12 antibody [RP23040104] (ab313434)")
Immunocytochemistry/ Immunofluorescence - Anti-ATG12 antibody [RP23040104] (ab313434)
Immunofluorescent analysis of HeLa cells fixed using 4% formaldehyde (reconstituted in 1X PBS) for 10 min at room temperature and permeabilized using 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling ATG12 with ab313434 at 2 ?g/mL followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate at 1/2000 dilution. Panel a) shows representative cells that were stained for detection and localization of ATG12 protein (green), Panel b) is stained for nuclei (blue) using DAPI. Panel c) represents cytoskeletal F-actin staining using Alexa Fluor 555 Rhodamine Phalloidin at 1/300 dilution. Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of ATG12 Panel e) represents control cells with no primary Antibody to assess background.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com