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AB313434

Anti-ATG12 antibody [RP23040104]

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Rabbit Recombinant Multiclonal ATG12 antibody. Suitable for WB, ICC/IF and reacts with Human, Mouse samples. Immunogen corresponding to Synthetic Peptide within Human ATG12.

View Alternative Names

APG12, APG12L, ATG12, Ubiquitin-like protein ATG12, Autophagy-related protein 12, APG12-like

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-ATG12 antibody [RP23040104] (AB313434)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ATG12 antibody [RP23040104] (AB313434)

Immunofluorescent analysis of HeLa cells fixed using 4% formaldehyde (reconstituted in 1X PBS) for 10 min at room temperature and permeabilized using 0.1 % Triton X-100 in PBS for 15 min at room temperature labeling ATG12 with ab313434 at 2 µg/mL followed by Goat anti-Rabbit IgG (H+L) Secondary Antibody, Alexa Fluor 488 conjugate at 1/2000 dilution. Panel a) shows representative cells that were stained for detection and localization of ATG12 protein (green), Panel b) is stained for nuclei (blue) using DAPI. Panel c) represents cytoskeletal F-actin staining using Alexa Fluor 555 Rhodamine Phalloidin at 1/300 dilution. Panel d) is a composite image of Panels a, b and c clearly demonstrating cytoplasmic localization of ATG12 Panel e) represents control cells with no primary Antibody to assess background.

Western blot - Anti-ATG12 antibody [RP23040104] (AB313434)
  • WB

Supplier Data

Western blot - Anti-ATG12 antibody [RP23040104] (AB313434)

All lanes:

Western blot - Anti-ATG12 antibody [RP23040104] (ab313434) at 4 µg/mL

Lane 1:

HEK293 whole cell lysate at 30 µg

Lane 2:

THP-1 whole cell lysate at 30 µg

Lane 3:

PANC-1 whole cell lysate at 30 µg

Lane 4:

HCT-116 whole cell lysate at 30 µg

Lane 5:

C2C12 whole cell lysate at 30 µg

Lane 6:

NIH 3T3 whole cell lysate at 30 µg

Lane 7:

Mouse colon tissue lysate at 30 µg

Lane 8:

Mouse Kidney tissue lysate at 30 µg

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/2500 dilution

Predicted band size: 15.113 kDa

Observed band size: 55 kDa

true

Western blot - Anti-ATG12 antibody [RP23040104] (AB313434)
  • WB

Supplier Data

Western blot - Anti-ATG12 antibody [RP23040104] (AB313434)

All lanes:

Western blot - Anti-ATG12 antibody [RP23040104] (ab313434) at 1/350 dilution

Lane 1:

Untransfected U-2OS cell lysate

Lane 2:

non-targeting scrambled siRNA transfected U-2OS cell lysate

Lane 3:

ATG12 knockdown u-2OS cell lysate

Secondary

All lanes:

Goat anti-Rabbit IgG (H+L) Secondary Antibody, HRP conjugate at 1/4000 dilution

Predicted band size: 15.113 kDa

Observed band size: 55 kDa

false

Key facts

Host species

Rabbit

Clonality

Multiclonal

Clone number

RP23040104

Isotype

IgG

Carrier free

No

Reacts with

Human, Mouse

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human ATG12. Database link O94817

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2.5-4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "2 µg/mL", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "2.5-4 µg/mL", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Product details

What are recombinant multiclonals?
Recombinant multiclonals are a mixture of recombinant antibodies co-expressed from a library of heavy and light chains. They offer several advantages including:

  • - The sensitivity of polyclonal antibodies by recognising multiple epitopes
  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

View our range of recombinant multiclonal antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 Preservative: 0.09% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATG12 also known as Autophagy-related protein 12 plays a critical role in autophagy an essential cellular process for degrading and recycling cellular components. The molecular weight of ATG12 is around 14 kDa. It is part of the ATG12-conjugation system and is invariably expressed in various tissues with notable presence in the liver brain and heart. ATG12 forms a covalent bond with ATG5 in a ubiquitin-like conjugation pathway which is significant for this mechanism.
Biological function summary

ATG12 is vital for the formation of autophagosomes which are double-membraned vesicles that sequester cellular components for degradation. It functions as part of a complex by binding to ATG5 and further associates with ATG16L1 to form a larger ATG12-ATG5-ATG16 complex. This complex localizes to the outer membrane of the growing autophagosome facilitating elongation and closure of the vesicle making it effective in the regulation of autophagy.

Pathways

ATG12's interaction with other proteins like ATG5 places it in the macroautophagy pathway an important process maintaining cellular homeostasis and turnover of cellular components. In addition to this ATG12 also contributes to cellular responses to stress conditions via this pathway. Furthermore it has connections with signaling pathways involving proteins such as LC3 which marks autophagosomal membranes and interacts with ATG12-conjugated complexes enhancing the autophagic flux.

Researchers associate dysregulated expression of ATG12 with neurodegenerative diseases such as Parkinson’s disease where impaired autophagy leads to toxic protein accumulation. Additionally alterations in ATG12 expression have links to cancer as enhanced autophagy in some tumor cells supports survival under metabolic stress. Both disorders demonstrate connections to proteins involved in the autophagy pathway suggesting therapeutic potential in modulating ATG12 activity.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes. As part of the ATG8 conjugation system with ATG5 and ATG16L1, required for recruitment of LRRK2 to stressed lysosomes and induction of LRRK2 kinase activity in response to lysosomal stress (By similarity).. (Microbial infection) May act as a proviral factor. In association with ATG5, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection (PubMed : 17709747). Required for the translation of incoming hepatitis C virus (HCV) RNA and, thereby, for the initiation of HCV replication, but not required once infection is established (PubMed : 19666601).
See full target information ATG12
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Product promise

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For full details, please see our Terms & Conditions

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