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Knockout Tested Rabbit Recombinant Monoclonal ATG14L antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

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Images

Flow Cytometry (Intracellular) - Anti-ATG14 antibody [EPR26188-66] - BSA and Azide free (AB315010), expandable thumbnail
  • Western blot - Anti-ATG14 antibody [EPR26188-66] - BSA and Azide free (AB315010), expandable thumbnail
  • Western blot - Anti-ATG14 antibody [EPR26188-66] - BSA and Azide free (AB315010), expandable thumbnail
  • Western blot - Anti-ATG14 antibody [EPR26188-66] - BSA and Azide free (AB315010), expandable thumbnail
  • Western blot - Anti-ATG14 antibody [EPR26188-66] - BSA and Azide free (AB315010), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: 100% PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBFlow Cyt (Intra)IHC-PICC/IFIP
Human
Tested
Tested
Not recommended
Not recommended
Not recommended
Mouse
Tested
Expected
Not recommended
Not recommended
Not recommended
Rat
Tested
Expected
Not recommended
Not recommended
Not recommended

Tested
Tested

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

1 product for Alternative Version

Target data

Function

Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1 (PubMed:18843052, PubMed:19050071). Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine (PubMed:19270696, PubMed:20713597). Promotes BECN1 translocation from the trans-Golgi network to autophagosomes (PubMed:20713597). Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1 (PubMed:23878393). Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK (PubMed:23878393). Binds to STX17-SNAP29 binary t-SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion (PubMed:25686604, PubMed:37632749). Modulates the hepatic lipid metabolism (By similarity).

Alternative names

ATG14L, KIAA0831, ATG14, Beclin 1-associated autophagy-related key regulator, Barkor, Autophagy-related protein 14-like protein, Atg14L

Recommended products

Knockout Tested Rabbit Recombinant Monoclonal ATG14L antibody. Carrier free. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR26188-66
Purification technique
Affinity purification Protein A
Specificity

In western blot we detect a double banding pattern. Based on the location of the immunogen, this antibody recognises both ATG14L and ATG14S isoforms (PMID: 36371383).

Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C

Notes

ab315010 is the carrier-free version of Anti-ATG14 antibody [EPR26188-66] ab315009.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ATG14 also known as Barkor is an important component involved in autophagy a cellular process for degrading and recycling cellular components. The protein has an approximate mass of 55 kDa. Researchers have observed ATG14 expression in various tissues with particularly high levels in the brain and liver. It plays a significant role in the formation and maturation of autophagosomes which are double-membrane structures that capture unnecessary cellular material for degradation.

Biological function summary

ATG14 serves as an important regulator within the autophagy machinery. It functions as a part of the Beclin-1-VPS34 complex which is essential for initiating the nucleation of the autophagosome membrane. This complex facilitates the localization of other autophagy-related proteins to the pre-autophagosomal structures thereby aiding in assembling the autophagosome. Through its ability to coordinate this assembly ATG14 influences both cellular homeostasis and response to stress.

Pathways

Researchers place ATG14 at a central point in the autophagy signaling pathway. It has connections to the PI3K signaling pathway where it interacts with Beclin-1 and VPS34 proteins. ATG14's function ties into the nutrient-sensing pathways that regulate cell growth and metabolism particularly under conditions of cellular stress or nutrient deprivation. This involvement reveals its relevance to cellular adaptive mechanisms which are important for organisms to maintain balance under varying environmental conditions.

Associated diseases and disorders

ATG14 associates with neurodegenerative diseases and certain types of cancer. Its role in autophagy suggests influence on the development and progression of neurodegenerative disorders like Alzheimer's disease where malfunctioning autophagy leads to the accumulation of defective proteins. Furthermore aberrations in autophagy regulated by ATG14 may contribute to cancer progression through interactions with oncogenes and tumor suppressors such as Beclin-1. These associations underline ATG14’s potential as a target for therapeutic strategies in treating related diseases.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

5 product images

  • Flow Cytometry (Intracellular) - Anti-ATG14 antibody [EPR26188-66] - BSA and Azide free (ab315010), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ATG14 antibody [EPR26188-66] - BSA and Azide free (ab315010)

    This data was developed using Anti-ATG14 antibody [EPR26188-66] ab315009, the same antibody clone in a different buffer formulation.

    Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized ATG14 KO HeLa (human ATG14 knockout cervical adenocarcinoma epithelial cell, Left) / Parental HeLa (Right) cells labelling ATG14 with Anti-ATG14 antibody [EPR26188-66] ab315009 at 1/50 dilution (1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

  • Western blot - Anti-ATG14 antibody [EPR26188-66] - BSA and Azide free (ab315010), expandable thumbnail

    Western blot - Anti-ATG14 antibody [EPR26188-66] - BSA and Azide free (ab315010)

    This data was developed using Anti-ATG14 antibody [EPR26188-66] ab315009, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Anti-ATG14 antibody [EPR26188-66] ab315009 can detect both ATG14L and ATG14S isoforms (PMID: 36371383).

    In Western blot, Anti-ATG14 antibody [EPR26188-66] ab315009 was shown to bind specifically to ATG14. Target of interest was observed at 65 kDa and 58 KDa in wild-type HeLa cell lysates (lane 1) with no signal observed at this size in ATG14 knockout cell line (lane 2) (lane 2, knockout cell line Human ATG14 (ATG14L) knockout HeLa cell line ab264684 / knockout cell lysate Human ATG14 (ATG14L) knockout HeLa cell lysate ab258319).

    In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (Anti-GAPDH antibody [EPR16891] - Loading Control ab181602) staining at 1/200000 dilution.

    All lanes: Western blot - Anti-ATG14 antibody [EPR26188-66] (Anti-ATG14 antibody [EPR26188-66] ab315009) at 1/1000 dilution

    Lane 1: Wild-type HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: ATG14 knockout HeLa whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 65, 58 kDa, 36 kDa

    Exposure time: 81s

  • Western blot - Anti-ATG14 antibody [EPR26188-66] - BSA and Azide free (ab315010), expandable thumbnail

    Western blot - Anti-ATG14 antibody [EPR26188-66] - BSA and Azide free (ab315010)

    This data was developed using Anti-ATG14 antibody [EPR26188-66] ab315009, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: Intercept® (TBS) Blocking Buffer diluted with an equal volume of TBS.

    Anti-ATG14 antibody [EPR26188-66] ab315009 can detect both ATG14L and ATG14S isoforms (PMID: 36371383).

    In lanes 2 and 4, the lysate was stored at -80℃ prior to Western Blotting. In lanes 1 and 3, lysate was freshly made and used for Western Blotting immediately to minimize protein degradation.

    The samples were run on a Bis-Tris gel under reducing conditions.

    Anti-ATG14 antibody (Anti-ATG14 antibody [EPR26188-66] ab315009) staining at 1/1000 dilution, shown in green; Mouse anti-GAPDH antibody 6C5 (Anti-GAPDH antibody [6C5] - Loading Control ab8245) loading control staining at 1/20000 dilution, shown in red.

    All lanes: Western blot - Anti-ATG14 antibody [EPR26188-66] (Anti-ATG14 antibody [EPR26188-66] ab315009) at 1/1000 dilution

    Lane 1: Fresh HCT 116 (human colorectal carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: Frozen HCT 116 whole cell lysate at 20 µg

    Lane 3: Fresh HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 4: Frozen HepG2 whole cell lysate at 20 µg

    Secondary

    Lanes 1 - 4: Goat Anti-Rabbit IgG H&L (800CW) at 1/20000 dilution

    Lanes 1 - 4: Goat Anti-Mouse IgG H&L (680RD) at 1/20000 dilution

    Performed under reducing conditions.

    Observed band size: 58, 65 kDa, 36 kDa

  • Western blot - Anti-ATG14 antibody [EPR26188-66] - BSA and Azide free (ab315010), expandable thumbnail

    Western blot - Anti-ATG14 antibody [EPR26188-66] - BSA and Azide free (ab315010)

    This data was developed using Anti-ATG14 antibody [EPR26188-66] ab315009, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Anti-ATG14 antibody [EPR26188-66] ab315009 can detect both ATG14L and ATG14S isoforms (PMID: 36371383).

    All lanes: Western blot - Anti-ATG14 antibody [EPR26188-66] (Anti-ATG14 antibody [EPR26188-66] ab315009) at 1/1000 dilution

    Lane 1: Mouse brain tissue lysate at 20 µg

    Lane 2: Mouse testis tissue lysate at 20 µg

    Lane 3: Mouse pancreas tissue lysate at 20 µg

    Lane 4: Neuro-2a (mouse neuroblastoma neuroblast) whole cell lysate at 20 µg

    Lane 5: Rat brain tissue lysate at 20 µg

    Lane 6: Rat testis tissue lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 58, 65 kDa

    Exposure time: 81s

  • Western blot - Anti-ATG14 antibody [EPR26188-66] - BSA and Azide free (ab315010), expandable thumbnail

    Western blot - Anti-ATG14 antibody [EPR26188-66] - BSA and Azide free (ab315010)

    This data was developed using Anti-ATG14 antibody [EPR26188-66] ab315009, the same antibody clone in a different buffer formulation.

    Blocking and diluting buffer and concentration: 5% NFDM/TBST.

    Anti-ATG14 antibody [EPR26188-66] ab315009 can detect both ATG14L and ATG14S isoforms (PMID: 36371383).

    Lysates were freshly made and used for Western blotting immediately to minimize protein degradation.

    All lanes: Western blot - Anti-ATG14 antibody [EPR26188-66] (Anti-ATG14 antibody [EPR26188-66] ab315009) at 1/1000 dilution

    Lane 1: HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

    Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg

    Lane 3: NIH/3T3 (mouse embryonic fibroblast) whole cell lysate at 20 µg

    Lane 4: PC-12 (rat adrenal gland pheochromocytoma cell) whole cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

    Observed band size: 58, 65 kDa

    Exposure time: 59s

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