Rabbit Recombinant Monoclonal ATG14L antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Images
![Sandwich ELISA - Anti-ATG14L antibody [EPR28551-51] - BSA and Azide free (Detector) (AB322649), expandable thumbnail](https://content.abcam.com/products/images/atg14l-antibody-epr28551-51-bsa-and-azide-free-detector-ab322649--sandwich-elisa-img439361.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-ATG14L antibody [EPR28551-51] - BSA and Azide free (Detector) (AB322649)")
![Sandwich ELISA - Anti-ATG14L antibody [EPR28551-51] - BSA and Azide free (Detector) (AB322649), expandable thumbnail](https://content.abcam.com/products/images/atg14l-antibody-epr28551-51-bsa-and-azide-free-detector-ab322649--sandwich-elisa-img439362.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-ATG14L antibody [EPR28551-51] - BSA and Azide free (Detector) (AB322649)")
![Sandwich ELISA - Anti-ATG14L antibody [EPR28551-51] - BSA and Azide free (Detector) (AB322649), expandable thumbnail](https://content.abcam.com/products/images/atg14l-antibody-epr28551-51-bsa-and-azide-free-detector-ab322649--sandwich-elisa-img439363.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-ATG14L antibody [EPR28551-51] - BSA and Azide free (Detector) (AB322649)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Human | Tested |
sELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Target data
Function
Required for both basal and inducible autophagy. Determines the localization of the autophagy-specific PI3-kinase complex PI3KC3-C1 (PubMed:18843052, PubMed:19050071). Plays a role in autophagosome formation and MAP1LC3/LC3 conjugation to phosphatidylethanolamine (PubMed:19270696, PubMed:20713597). Promotes BECN1 translocation from the trans-Golgi network to autophagosomes (PubMed:20713597). Enhances PIK3C3 activity in a BECN1-dependent manner. Essential for the autophagy-dependent phosphorylation of BECN1 (PubMed:23878393). Stimulates the phosphorylation of BECN1, but suppresses the phosphorylation PIK3C3 by AMPK (PubMed:23878393). Binds to STX17-SNAP29 binary t-SNARE complex on autophagosomes and primes it for VAMP8 interaction to promote autophagosome-endolysosome fusion (PubMed:25686604, PubMed:37632749). Modulates the hepatic lipid metabolism (By similarity).
Alternative names
ATG14L, KIAA0831, ATG14, Beclin 1-associated autophagy-related key regulator, Barkor, Autophagy-related protein 14-like protein, Atg14L
Rabbit Recombinant Monoclonal ATG14L antibody. Carrier free. Suitable for sELISA and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR28551-51
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab322649 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human ATG14L. The recommended pair for sandwich ELISA is:
Capture: Anti-ATG14L antibody [EPR28551-44] - BSA and Azide free (Capture) ab322648, Human ATG14L Capture Antibody (unconjugated)
Detector: ab322649, Human ATG14L Detector Antibody (unconjugated)
The reference range value is 15.63-2000 pg/mL.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Sandwich ELISA - Anti-ATG14L antibody [EPR28551-51] - BSA and Azide free (Detector) (ab322649), expandable thumbnail](https://content.abcam.com/products/images/atg14l-antibody-epr28551-51-bsa-and-azide-free-detector-ab322649--sandwich-elisa-img439361.jpg "Sandwich ELISA - Anti-ATG14L antibody [EPR28551-51] - BSA and Azide free (Detector) (ab322649)")
Sandwich ELISA - Anti-ATG14L antibody [EPR28551-51] - BSA and Azide free (Detector) (ab322649)
Sandwich ELISA of Human ATG14L Antibody Pair - BSA and Azide free ab322647 with the capture antibody Anti-ATG14L antibody [EPR28551-44] - BSA and Azide free (Capture) ab322648 dilution at 2 ug/mL and detector antibody ab322649 dilution at 0.5 ug/mL.
![Sandwich ELISA - Anti-ATG14L antibody [EPR28551-51] - BSA and Azide free (Detector) (ab322649), expandable thumbnail](https://content.abcam.com/products/images/atg14l-antibody-epr28551-51-bsa-and-azide-free-detector-ab322649--sandwich-elisa-img439362.jpg "Sandwich ELISA - Anti-ATG14L antibody [EPR28551-51] - BSA and Azide free (Detector) (ab322649)")
Sandwich ELISA - Anti-ATG14L antibody [EPR28551-51] - BSA and Azide free (Detector) (ab322649)
Interpolated concentrations of native ATG14L in human control wild type HeLa (human cervical adenocarcinoma epithelial cell) and ATG14 (ATG14L) knockout HeLa cell based on 250 ug/mL extract loads. The concentrations of ATG14L were measured in duplicate and interpolated from the ATG14L standard curve and corrected for sample dilution. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean ATG14L concentration was determined to be 397.4 pg/mL in wild type HeLa extract (Human wild-type HeLa cell line ab255448) and 50.4 pg/mL in ATG14 (ATG14L) knockout HeLa extract (Human ATG14 (ATG14L) knockout HeLa cell line Human ATG14 (ATG14L) knockout HeLa cell line ab264684).
![Sandwich ELISA - Anti-ATG14L antibody [EPR28551-51] - BSA and Azide free (Detector) (ab322649), expandable thumbnail](https://content.abcam.com/products/images/atg14l-antibody-epr28551-51-bsa-and-azide-free-detector-ab322649--sandwich-elisa-img439363.jpg "Sandwich ELISA - Anti-ATG14L antibody [EPR28551-51] - BSA and Azide free (Detector) (ab322649)")
Sandwich ELISA - Anti-ATG14L antibody [EPR28551-51] - BSA and Azide free (Detector) (ab322649)
Interpolated concentration of native ATG14L was measured at different sample concentrations. Undiluted samples are as follows: HeLa cell extract (250 ug/mL) and HCT116 cell extract (400 ug/mL). The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). Sample dilutions are made in antibody diluent CP2 (Antibody Diluent CP2 ab319981). Data represented here are indicative of assay working using example of biological sample. However, we do not guarantee same sensitivity level due to biological variation.
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