AB233796

Anti-ATG16L1 antibody [5H9A11]

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(1 Publication)

Mouse Monoclonal ATG16L1 antibody. Suitable for Flow Cyt, WB and reacts with Human, Mouse, Rat, Transfected cell lysate - Human, Recombinant fragment - Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human ATG16L1 aa 1-300.

View Alternative Names

APG16L, UNQ9393/PRO34307, ATG16L1, Autophagy-related protein 16-1, APG16-like 1

8 Images

Flow Cytometry - Anti-ATG16L1 antibody [5H9A11] (AB233796)

Flow Cyt

Supplier Data

Flow Cytometry - Anti-ATG16L1 antibody [5H9A11] (AB233796)

Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling ATG16L1 with ab233796 at 1/200 dilution (green) compared with a negative control (red).

Unknown

Western blot - Anti-ATG16L1 antibody [5H9A11] (AB233796)

Lanes 1-4 : Merged signal (red and green). Green - ab233796 observed at 68 and 72 kDa. Red - loading control ab181602 observed at 37 kDa.

ab233796 Anti-ATG16L1 antibody [5H9A11] was shown to specifically react with ATG16L1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261772 (knockout cell lysate ab256843) was used. Wild-type and ATG16L1 knockout samples were subjected to SDS-PAGE. ab233796 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ATG16L1 antibody [5H9A11] (ab233796) at 1/500 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

ATG16L1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human ATG16L1 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-atg16l1-knockout-hela-cell-line-ab261772'>ab261772</a>)

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

Daudi cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Mouse IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-mouse-igg-h-l-irdye-800cw-preadsorbed-ab216772'>ab216772</a>) at 1/20000 dilution

Predicted band size: 68 kDa

Observed band size: 68 kDa,72 kDa

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Lab

Western blot - Anti-ATG16L1 antibody [5H9A11] (AB233796)

False colour image of Western blot : Anti-ATG16L1 antibody [5H9A11] staining at 1/500 dilution, shown in green; Rabbit Anti-GAPDH antibody [EPR16891] (ab181602) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab233796 was shown to bind specifically to ATG16L1. A band was observed at 68/70 kDa in wild-type THP-1 cell lysates with no signal observed at this size in ATG16L1 knockout cell line ab277834 (knockout cell lysate ab278184). To generate this image, wild-type and ATG16L1 knockout THP-1 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in fluorescent western blot (TBS-based) blocking solution before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Mouse IgG H&L (IRDye^® 800CW) preabsorbed (ab216772) and Goat anti-Rabbit IgG H&L (IRDye^® 680RD) preabsorbed (ab216777) at 1/20000 dilution.

All lanes:

Western blot - Anti-ATG16L1 antibody [5H9A11] (ab233796) at 1/500 dilution

Lane 1:

Wild-type THP-1 cell lysate at 20 µg

Lane 2:

ATG16L1 knockout THP-1 cell lysate at 20 µg

Lane 2:

Western blot - Human ATG16L1 knockout THP-1 cell line (<a href='/en-us/products/cell-lines/human-atg16l1-knockout-thp-1-cell-line-ab277834'>ab277834</a>)

Lane 3:

Wild type HeLa cell lysate at 20 µg

Lane 4:

ATG16L1 knockout HeLa cell lysate at 20 µg

Predicted band size: 68 kDa

Observed band size: 68 kDa,70 kDa

false

Lab

Western blot - Anti-ATG16L1 antibody [5H9A11] (AB233796)

Lanes 1-4 : Merged signal (red and green). Green - ab233796 observed at 68 and 72 kDa. Red - loading control ab181602 observed at 37 kDa.

ab233796 Anti-ATG16L1 antibody [5H9A11] was shown to specifically react with ATG16L1 in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab261773 (knockout cell lysate ab256844) was used. Wild-type and ATG16L1 knockout samples were subjected to SDS-PAGE. ab233796 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^® 800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye^® 680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ATG16L1 antibody [5H9A11] (ab233796) at 1/500 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

ATG16L1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human ATG16L1 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-atg16l1-knockout-hela-cell-line-ab261773'>ab261773</a>)

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

Daudi cell lysate at 20 µg

Secondary

All lanes:

Predicted band size: 68 kDa

Observed band size: 68 kDa,72 kDa

false

Unknown

Western blot - Anti-ATG16L1 antibody [5H9A11] (AB233796)

Lanes 1- 4 : Merged signal (red and green). Green - ab233796 observed at 68 kDa. Red - Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) observed at 37 kDa.

ab233796 was shown to react with ATG16L1 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265263 (knockout cell lysate ab256842) was used. Wild-type HeLa and ATG16L1 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab233796 and Anti-GAPDH antibody[EPR16891] - Loading Control (ab181602) overnight at 4°C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Mouse IgG H&L (IRDye^®800CW) preadsorbed (ab216772) and Goat Anti-Rabbit IgG H&L (IRDye^®680RD) preadsorbed (ab216777) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ATG16L1 antibody [5H9A11] (ab233796) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

ATG16L1 knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human ATG16L1 knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-atg16l1-knockout-hela-cell-line-ab265263'>ab265263</a>)

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

Daudi cell lysate at 20 µg

Predicted band size: 68 kDa

Observed band size: 68 kDa

false

Supplier Data

Western blot - Anti-ATG16L1 antibody [5H9A11] (AB233796)

All lanes:

Western blot - Anti-ATG16L1 antibody [5H9A11] (ab233796) at 1/500 dilution

Lane 1:

HeLa (human epithelial cell line from cervix adenocarcinoma) cell lysate

Lane 2:

Raji (human Burkitt's lymphoma cell line) cell lysate

Lane 3:

PANC-1 (human pancreatic epithelial cancinoma cell line) cell lysate

Lane 4:

Jurkat (human T cell leukemia cell line from peripheral blood) cell lysate

Lane 5:

PC-12 (rat adrenal gland pheochromocytoma cell line) cell lysate

Lane 6:

HepG2 (human liver hepatocellular carcinoma cell line) cell lysate

Lane 7:

HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 8:

NIH/3T3 (mouse embryo fibroblast cell line) cell lysate

Predicted band size: 68 kDa

false

Supplier Data

Western blot - Anti-ATG16L1 antibody [5H9A11] (AB233796)

All lanes:

Western blot - Anti-ATG16L1 antibody [5H9A11] (ab233796) at 1/500 dilution

Lane 1:

Untransfected HEK-293 (human epithelial cell line from embryonic kidney) cell lysate

Lane 2:

ATG16L1 (aa 11-257)-hIgG-Fc-transfected HEK-293 cell lysate

Predicted band size: 68 kDa

false

Supplier Data

Western blot - Anti-ATG16L1 antibody [5H9A11] (AB233796)

Expected MW is 56 kDa.

All lanes:

Western blot - Anti-ATG16L1 antibody [5H9A11] (ab233796) at 1/500 dilution

All lanes:

Recombinant human ATG16L1 (aa 11-257) protein

Predicted band size: 68 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

5H9A11

Isotype

IgG1

Carrier free

Reacts with

Mouse, Rat, Human

Applications

WB, Flow Cyt

applications

Immunogen

Recombinant Fragment Protein within Human ATG16L1 aa 1-300. The exact immunogen used to generate this antibody is proprietary information.

Q676U5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/200 - 1/400", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Rat": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Recombinant fragment - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" }, "Transfected cell lysate - Human": { "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

Purified from tissue culture supernatant.

Storage buffer

Preservative: 0.05% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATG16L1 also known as Autophagy Related 16 Like 1 is a protein involved in the autophagy process. It functions as part of a complex that includes ATG12 and ATG5. The molecular weight of ATG16L1 is approximately 66 kDa. It is highly expressed in various tissues including the immune cells highlighting its involvement in essential cellular processes. The protein interacts with other autophagy-related proteins to facilitate the elongation and maturation of autophagosomes.

Biological function summary

ATG16L1 plays a role in mediating autophagy a vital cellular degradation process. It forms a complex with ATG5 and ATG12 necessary for the elongation of the autophagosome membrane. Apart from its role in autophagy ATG16L1 contributes to the regulation of innate immunity by influencing the secretion of inflammatory cytokines. Its presence is essential for maintaining cellular homeostasis and proper immune responses.

Pathways

ATG16L1 is important in pathways like autophagy and immunity. In the autophagy pathway it works alongside ATG5 and ATG12 to ensure the proper formation of autophagosomes which are structures that engulf and degrade unwanted cellular components. Furthermore in the immune response pathway it helps regulate inflammation by managing cytokine production and secretion showing interaction with proteins like NOD2.

ATG16L1 has connections with inflammatory bowel diseases particularly Crohn's disease and various cancers. Mutations in ATG16L1 can lead to impaired autophagy contributing to the development of Crohn's disease. In cancer dysregulation of autophagy involving ATG16L1 may affect tumor progression and response to therapy. The protein associates with NOD2 in Crohn's disease demonstrating that changes in their interaction can influence disease susceptibility and severity.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Plays an essential role in both canonical and non-canonical autophagy : interacts with ATG12-ATG5 to mediate the lipidation to ATG8 family proteins (MAP1LC3A, MAP1LC3B, MAP1LC3C, GABARAPL1, GABARAPL2 and GABARAP) (PubMed : 23376921, PubMed : 23392225, PubMed : 24553140, PubMed : 24954904, PubMed : 27273576, PubMed : 29317426, PubMed : 30778222, PubMed : 33909989). Acts as a molecular hub, coordinating autophagy pathways via distinct domains that support either canonical or non-canonical signaling (PubMed : 29317426, PubMed : 30778222). During canonical autophagy, interacts with ATG12-ATG5 to mediate the conjugation of phosphatidylethanolamine (PE) to ATG8 proteins, to produce a membrane-bound activated form of ATG8 (PubMed : 23376921, PubMed : 23392225, PubMed : 24553140, PubMed : 24954904, PubMed : 27273576). Thereby, controls the elongation of the nascent autophagosomal membrane (PubMed : 23376921, PubMed : 23392225, PubMed : 24553140, PubMed : 24954904, PubMed : 27273576). As part of the ATG8 conjugation system with ATG5 and ATG12, required for recruitment of LRRK2 to stressed lysosomes and induction of LRRK2 kinase activity in response to lysosomal stress (By similarity). Also involved in non-canonical autophagy, a parallel pathway involving conjugation of ATG8 proteins to single membranes at endolysosomal compartments, probably by catalyzing conjugation of phosphatidylserine (PS) to ATG8 (PubMed : 33909989). Non-canonical autophagy plays a key role in epithelial cells to limit lethal infection by influenza A (IAV) virus (By similarity). Regulates mitochondrial antiviral signaling (MAVS)-dependent type I interferon (IFN-I) production (PubMed : 22749352, PubMed : 25645662). Negatively regulates NOD1- and NOD2-driven inflammatory cytokine response (PubMed : 24238340). Instead, promotes an autophagy-dependent antibacterial pathway together with NOD1 or NOD2 (PubMed : 20637199). Plays a role in regulating morphology and function of Paneth cell (PubMed : 18849966).

See full target information ATG16L1

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Kidney international 81:458-68 PubMed22166849

2011

Functional metabotropic glutamate receptors 1 and 5 are expressed in murine podocytes.

Applications

Unspecified application

Species

Unspecified reactive species

Leyi Gu,Xinyue Liang,Lihua Wang,Yucheng Yan,Zhaohui Ni,Huili Dai,Jiayuan Gao,Shan Mou,Qin Wang,Xinyu Chen,Liming Wang,Jiaqi Qian

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATG16L1 antibody [5H9A11]

Flow Cytometry - Anti-ATG16L1 antibody [5H9A11] (AB233796)

Western blot - Anti-ATG16L1 antibody [5H9A11] (AB233796)

Western blot - Anti-ATG16L1 antibody [5H9A11] (AB233796)

Western blot - Anti-ATG16L1 antibody [5H9A11] (AB233796)

Western blot - Anti-ATG16L1 antibody [5H9A11] (AB233796)

Western blot - Anti-ATG16L1 antibody [5H9A11] (AB233796)

Western blot - Anti-ATG16L1 antibody [5H9A11] (AB233796)

Western blot - Anti-ATG16L1 antibody [5H9A11] (AB233796)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise