AB226931

Anti-ATG2A antibody

Name: Anti-ATG2A antibody (ab226931) | Abcam
Brand: Abcam Limited
SKU: AB226931
Price: 525 USD
Availability: InStock

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(3 Publications)

Rabbit Polyclonal ATG2A antibody. Suitable for IP, WB and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ATG2A.

View Alternative Names

KIAA0404, ATG2A, Autophagy-related protein 2 homolog A

2 Images

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Immunoprecipitation - Anti-ATG2A antibody (AB226931)

ATG2A was immunoprecipitated from THP-1 (human monocytic leukemia cell line) whole cell extract with 5 μg ab226931. Western blot was performed from the immunoprecipitate using ab226931.

Lane 1 : Control IgG IP in THP-1 whole cell extract.

Lane 2 : ab226931 IP in THP-1 whole cell extract.

All lanes:

Immunoprecipitation - Anti-ATG2A antibody (ab226931)

Predicted band size: 212 kDa

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Supplier Data

Western blot - Anti-ATG2A antibody (AB226931)

5% SDS-PAGE gel.

All lanes:

Western blot - Anti-ATG2A antibody (ab226931) at 1/1000 dilution

Lane 1:

U-87 MG (human glioblastoma-astrocytoma epithelial cell line) whole cell extract at 30 µg

Lane 2:

SK-N-SH (human neuroblastoma cell line) whole cell extract at 30 µg

Predicted band size: 212 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Recombinant Fragment Protein within Human ATG2A. The exact immunogen used to generate this antibody is proprietary information.

Q2TAZ0

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/571", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 Preservative: 0.025% Proclin 300 Constituents: PBS, 20% Glycerol (glycerin, glycerine)

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATG2A also known as Autophagy-related protein 2 homolog A is an important protein involved in autophagy. It has a molecular mass of approximately 230 kDa and is expressed in tissues like the liver heart and skeletal muscle. Mechanically ATG2A is important for the lipid transfer between membranes which is essential for the expansion of autophagosomes cellular structures that engulf and degrade damaged cellular materials.

Biological function summary

ATG2A plays an important role in macroautophagy by forming part of a multiprotein complex called the ATG9 complex. It participates in membrane tethering and expansion during autophagosome formation. This protein interacts with other autophagy-related proteins including the ATG8 family to facilitate the progression of the autophagic process thereby maintaining cellular health and homeostasis.

Pathways

ATG2A operates mainly within the autophagy pathway specifically the PI3K-AKT-mTOR signaling pathway which is vital for cellular survival and growth. In this context ATG2A acts in concert with proteins like ULK1 which helps initiate autophagy. The regulation of autophagic flux via ATG2A is critical for responding to cellular stress and adjusting metabolic activities according to the environmental conditions.

Mutations or dysfunction in ATG2A can impact cancer and neurodegenerative disorders such as Alzheimer's disease. The protein's role in cancer links to its association with protein components like Beclin 1 which regulates autophagic vacuole formation and influences cancer cell survival. In Alzheimer's disease ATG2A interactions with neural proteins can affect the degradation of misfolded proteins which is instrumental in disease progression.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Lipid transfer protein involved in autophagosome assembly (PubMed : 28561066, PubMed : 30952800, PubMed : 31271352). Tethers the edge of the isolation membrane (IM) to the endoplasmic reticulum (ER) and mediates direct lipid transfer from ER to IM for IM expansion (PubMed : 30952800, PubMed : 31271352). Binds to the ER exit site (ERES), which is the membrane source for autophagosome formation, and extracts phospholipids from the membrane source and transfers them to ATG9 (ATG9A or ATG9B) to the IM for membrane expansion (PubMed : 30952800, PubMed : 31271352). Lipid transfer activity is enhanced by WIPI1 and WDR45/WIPI4, which promote ATG2A-association with phosphatidylinositol 3-monophosphate (PI3P)-containing membranes (PubMed : 31271352). Also regulates lipid droplets morphology and distribution within the cell (PubMed : 22219374, PubMed : 28561066).

See full target information ATG2A

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Journal of neuroinflammation 22:113 PubMed40254576

2025

Targeting SARM1 as a novel neuroprotective therapy in neurotropic viral infections.

Applications

Unspecified application

Species

Unspecified reactive species

Sheng He,Yanyan Zhu,Xinyue Wang,Gaofeng Zhang,Kaijian Hou,Xianzhu Xia,Zhenyou Jiang,Xiaoqian Gong,Pingsen Zhao

Autophagy 18:2615-2635 PubMed35253629

2022

Therapeutic targeting of the USP2-E2F4 axis inhibits autophagic machinery essential for zinc homeostasis in cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Xiao,Jianqun Wang,Xiaojing Wang,Shuang Cai,Yanhua Guo,Lin Ye,Dan Li,Anpei Hu,Shikai Jin,Boling Yuan,Yi Zhou,Qilan Li,Qiangsong Tong,Liduan Zheng

Autophagy 17:3644-3670 PubMed33685363

2021

WIPI1 promotes fission of endosomal transport carriers and formation of autophagosomes through distinct mechanisms.

Applications

Unspecified application

Species

Unspecified reactive species

Maria Giovanna De Leo,Philipp Berger,Andreas Mayer

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ATG2A antibody

Immunoprecipitation - Anti-ATG2A antibody (AB226931)

Western blot - Anti-ATG2A antibody (AB226931)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise