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AB189934

Anti-ATG2B antibody - N-terminal

3

(1 Review)

|

(6 Publications)

Rabbit Polyclonal ATG2B antibody. N-terminal. Suitable for WB, IHC-P, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human ATG2B.

View Alternative Names

C14orf103, ATG2B, Autophagy-related protein 2 homolog B

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-ATG2B antibody - N-terminal (AB189934)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ATG2B antibody - N-terminal (AB189934)

Immunofluorescent analysis of K562 cells labeling ATG2B with ab189934 at 20 μg/ml.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG2B antibody - N-terminal (AB189934)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG2B antibody - N-terminal (AB189934)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human colon tissue labeling ATG2B with ab189934 at 5 μg/ml.

Western blot - Anti-ATG2B antibody - N-terminal (AB189934)
  • WB

Supplier Data

Western blot - Anti-ATG2B antibody - N-terminal (AB189934)

Lane 1:

Western blot - Anti-ATG2B antibody - N-terminal (ab189934) at 1 µg/mL

Lane 2:

Western blot - Anti-ATG2B antibody - N-terminal (ab189934) at 2 µg/mL

All lanes:

K562 cell lysate

Predicted band size: 233 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Synthetic Peptide within Human ATG2B. The exact immunogen used to generate this antibody is proprietary information.

Q96BY7

Specificity

ab189934 is predicted to not cross-react with other ATG2A.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1-2 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "20 µg/mL", "ICCIF-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATG2B also known as Autophagy-related protein 2 homolog B plays a significant role in the autophagy process. This protein encodes a part of the essential machinery for the expansion of the autophagosome which is important for cellular degradation and recycling of cytoplasmic components. ATG2B has a molecular mass of approximately 223 kDa and shows expression in various tissues with higher levels observed in the heart and liver. Scientists study ATG2B due to its important function in maintaining cellular homeostasis.
Biological function summary

The protein facilitates the transfer of lipids necessary for the elongation of the pre-autophagosomal structure. ATG2B acts as a critical component of the ATG protein complex interacting closely with ATG9A and WIPI1 proteins. Through this complex ATG2B supports the formation and growth of autophagic membranes playing a pivotal role in cellular stress responses. The protein also affects cargo recognition during autophagy thereby influencing the selectivity of molecules degraded within the lysosome.

Pathways

The autophagy process significantly involves ATG2B within its regulatory mechanisms. This protein interacts actively in the mechanistic target of rapamycin (mTOR) signaling pathway which controls autophagy initiation and progression based on cellular nutrient status. Furthermore ATG2B shares collaborations with other related proteins within the phosphatidylinositol 3-kinase (PI3K) pathway linking it to lipid signaling mediations. These associations reinforce its role in maintaining cell survival pathways by degradation of damaged organelles and proteins.

Scientists have linked ATG2B to conditions like cancer and neurodegenerative diseases. The malfunction or alteration of ATG2B functions may result in defective autophagy contributing to the growth and survival of cancer cells by disrupting normal cellular cleanup processes. Similarly impaired ATG2B-mediated autophagy can lead to the accumulation of dysfunctional proteins a hallmark of diseases like Parkinson's. Additionally there are associations between ATG2B and proteins such as alpha-synuclein in these neurodegenerative contexts indicating a potential role in the pathology of these disorders.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Lipid transfer protein required for both autophagosome formation and regulation of lipid droplet morphology and dispersion (PubMed : 22219374, PubMed : 31721365). Tethers the edge of the isolation membrane (IM) to the endoplasmic reticulum (ER) and mediates direct lipid transfer from ER to IM for IM expansion (PubMed : 22219374, PubMed : 31721365). Binds to the ER exit site (ERES), which is the membrane source for autophagosome formation, and extracts phospholipids from the membrane source and transfers them to ATG9 (ATG9A or ATG9B) to the IM for membrane expansion (By similarity). Lipid transfer activity is enhanced by WDR45/WIPI4, which promotes ATG2B-association with phosphatidylinositol 3-monophosphate (PI3P)-containing membranes (PubMed : 31721365).
See full target information ATG2B
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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 15:410 PubMed38866787

2024

Human gastric cancer progression and stabilization of ATG2B through RNF5 binding facilitated by autophagy-associated CircDHX8.

Applications

Unspecified application

Species

Unspecified reactive species

Guanxin Wei,Xiang Chen,Tuo Ruan,Xianxiong Ma,Xiuxian Zhu,Wenhao Wen,Danzeng He,Kaixiong Tao,Chuanqing Wu

Bioengineered 12:4569-4580 PubMed34320900

2021

Crocin exerts anti-proliferative and apoptotic effects on cutaneous squamous cell carcinoma via miR-320a/ATG2B.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoqing Bi,Zhenjuan Jiang,Zhaohui Luan,Daoqing Qiu

Journal of cellular and molecular medicine 25:8062-8073 PubMed34180119

2021

The lncRNA HOTAIR regulates autophagy and affects lipopolysaccharide-induced acute lung injury through the miR-17-5p/ATG2/ATG7/ATG16 axis.

Applications

Unspecified application

Species

Unspecified reactive species

Yujun Li,Zhike Liang,Hua He,Xiaomei Huang,Zexun Mo,Jinwen Tan,Weihong Guo,Ziwen Zhao,Shuquan Wei

Cell death & disease 12:367 PubMed33824300

2021

LncRNA-HOTAIR activates autophagy and promotes the imatinib resistance of gastrointestinal stromal tumor cells through a mechanism involving the miR-130a/ATG2B pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Jinyan Zhang,Ke Chen,Yuexiao Tang,Xiaorui Luan,Xiaoxiao Zheng,Xuemei Lu,Jiayan Mao,Liqiang Hu,Shufen Zhang,Xianning Zhang,Wei Chen

Tissue & cell 67:101453 PubMed33130456

2020

MiR-143-3p targets ATG2B to inhibit autophagy and promote endothelial progenitor cells tube formation in deep vein thrombosis.

Applications

Unspecified application

Species

Unspecified reactive species

Wangao Zhang,Pengju Chen,Huimin Zong,Yikun Ding,Ruhu Yan

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research 26:2498-510 PubMed21681813

2011

Smad6 is essential to limit BMP signaling during cartilage development.

Applications

Unspecified application

Species

Unspecified reactive species

Kristine D Estrada,Kelsey N Retting,Alana M Chin,Karen M Lyons
View all publications
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Product promise

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