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Rabbit Recombinant Monoclonal ATG3 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

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Images

Flow Cytometry (Intracellular) - Anti-ATG3 antibody [EPR4802] (AB108282), expandable thumbnail
  • Western blot - Anti-ATG3 antibody [EPR4802] (AB108282), expandable thumbnail
  • Western blot - Anti-ATG3 antibody [EPR4802] (AB108282), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.05% Sodium azide
Constituents: 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 0.1% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBIHC-PICC/IFFlow Cyt (Intra)
Human
Tested
Not recommended
Not recommended
Tested
Mouse
Expected
Not recommended
Not recommended
Predicted
Rat
Expected
Not recommended
Not recommended
Predicted

Tested
Tested

Species
Human
Dilution info
1/1000 - 1/10000
Notes

-

Expected
Expected

Species
Mouse, Rat
Dilution info
Use at an assay dependent concentration.
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Mouse, Rat
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
1/10 - 1/100
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Predicted
Predicted

Species
Mouse, Rat
Dilution info
-
Notes

-

Associated Products

Select an associated product type

3 products for Alternative Product

Target data

Function

E2 conjugating enzyme that catalyzes the covalent conjugation of the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A) to the amino group of phosphatidylethanolamine (PE)-containing lipids in the membrane resulting in membrane-bound ATG8-like proteins which is one of the key steps in the development of autophagic isolation membranes during autophagosome formation (PubMed:24191030, PubMed:33446636, PubMed:37252361). Cycles back and forth between binding to ATG7 for loading with the ATG8-like proteins and binding to E3 enzyme, composed of ATG12, ATG5 and ATG16L1 to promote ATG8-like proteins lipidation (PubMed:11825910, PubMed:12207896, PubMed:12890687, PubMed:16704426, PubMed:24186333). Also plays a role as a membrane curvature sensor that facilitates LC3/GABARAP lipidation by sensing local membrane stress associated with lipid-packing defects as occurs with high molar proportions of conical lipids or strident membrane curvature (By similarity). Interacts with negatively-charged membranes promoting membrane tethering and enhancing LC3/GABARAP lipidation (PubMed:29142222). Also acts as an autocatalytic E2-like enzyme by catalyzing the conjugation of ATG12 to itself in an ATG7-dependent manner, this complex thus formed, plays a role in mitochondrial homeostasis but not in autophagy (By similarity). ATG12-ATG3 conjugation promotes late endosome to lysosome trafficking and basal autophagosome maturation via its interaction with PDCD6IP (By similarity). ATG12-ATG3 conjugate is also formed upon viccina virus infection, leading to the disruption the cellular autophagy which is not necessary for vaccinia survival and proliferation (By similarity). Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway (By similarity).

Alternative names

APG3, APG3L, ATG3, Ubiquitin-like-conjugating enzyme ATG3, Autophagy-related protein 3, Protein PC3-96, APG3-like, hApg3

Recommended products

Rabbit Recombinant Monoclonal ATG3 antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse, Rat samples. Cited in 5 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR4802
Purity
Tissue culture supernatant
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Stable for 12 months at -20°C

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ATG3 also known as Autophagy-related protein 3 is an essential enzyme in the autophagy process. Its molecular weight is approximately 36 kDa. This protein facilitates the conjugation of LC3 (microtubule-associated protein 1A/1B-light chain 3) by acting as an E2-like enzyme. It is mainly expressed in tissues with high rates of autophagy including liver heart and brain.

Biological function summary

ATG3 plays an important role in the elongation of the autophagic membrane. It operates as a part of the autophagy machinery working closely with ATG7 an E1-like enzyme to transfer phosphatidylethanolamine (PE) to LC3 converting it into LC3-II. This lipidated form of LC3 is essential in the expansion and closure of the isolation membrane which forms the autophagosome the core structure for degradation and recycling of cellular components.

Pathways

ATG3 is a central player in the macroautophagy pathway a process critical for cellular homeostasis. It interacts with ATG7 to facilitate the lipidation of ATG8 family proteins which are important for autophagosome formation. Moreover ATG3 also links to apoptosis pathways where its balance with pro-apoptotic proteins helps regulate cell death processes.

Associated diseases and disorders

ATG3 has been implicated in neurodegenerative diseases like Alzheimer's. This relates partly to its role in protein clearance which is essential for preventing toxic aggregations. Altered ATG3 function can lead to accumulation of misfolded proteins exacerbating disease. Moreover ATG3's interaction with proteins in the cancer-related pathways such as p53 shows its involvement in tumorigenesis through its influence on cell survival and death mechanisms.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • Flow Cytometry (Intracellular) - Anti-ATG3 antibody [EPR4802] (ab108282), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ATG3 antibody [EPR4802] (ab108282)

    Intracellular flow cytometric analysis of permeabilized Jurkat cells using ab108282 at 1/10 (red) or a rabbit IgG (negative) (green).

  • Western blot - Anti-ATG3 antibody [EPR4802] (ab108282), expandable thumbnail

    Western blot - Anti-ATG3 antibody [EPR4802] (ab108282)

    Lanes 1-4: Merged signal (red and green). Green - ab108282 observed at 40 kDa. Red - loading control Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291 observed at 50 kDa.

    ab108282 Anti-ATG3 antibody [EPR4802] was shown to specifically react with ATG3 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human ATG3 knockout HEK-293T cell line ab266707 (knockout cell lysate Human ATG3 knockout HEK-293T cell lysate ab257363) was used. Wild-type and ATG3 knockout samples were subjected to SDS-PAGE. ab108282 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-ATG3 antibody [EPR4802] (ab108282) at 1/1000 dilution

    Lane 1: Wild-type HEK293T cell lysate at 20 µg

    Lane 2: ATG3 knockout HEK293T cell lysate at 20 µg

    Lane 2: Western blot - Human ATG3 knockout HEK-293T cell line (Human ATG3 knockout HEK-293T cell line ab266707)

    Lane 3: K-562 cell lysate at 20 µg

    Lane 4: HeLa cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 35 kDa

    Observed band size: 40 kDa

  • Western blot - Anti-ATG3 antibody [EPR4802] (ab108282), expandable thumbnail

    Western blot - Anti-ATG3 antibody [EPR4802] (ab108282)

    All lanes: Western blot - Anti-ATG3 antibody [EPR4802] (ab108282) at 1/1000 dilution

    Lane 1: K562 cell lysate at 10 µg

    Lane 2: HeLa cell lysate at 10 µg

    Lane 3: Jurkat cell lysate at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 35 kDa

    Observed band size: 40 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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