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AB154843

Anti-ATG4B antibody [EPR6436(2)]

5

(2 Reviews)

|

(12 Publications)

Rabbit Recombinant Monoclonal ATG4B antibody. Suitable for WB, Flow Cyt (Intra) and reacts with Human, Mouse samples. Cited in 12 publications.

View Alternative Names

APG4B, AUTL1, KIAA0943, ATG4B, Cysteine protease ATG4B, AUT-like 1 cysteine endopeptidase, Autophagy-related cysteine endopeptidase 1, Autophagy-related protein 4 homolog B, Autophagin-1, HsAPG4B, hAPG4B

4 Images
Flow Cytometry (Intracellular) - Anti-ATG4B antibody [EPR6436(2)] (AB154843)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-ATG4B antibody [EPR6436(2)] (AB154843)

Intracellular Flow Cytometry analysis of permeabilized Jurkat cells labeling ATG4B (red), using ab154843 at a 1/100 dilution, and negative control cells probed with a Rabbit IgG (green)

Western blot - Anti-ATG4B antibody [EPR6436(2)] (AB154843)
  • WB

Lab

Western blot - Anti-ATG4B antibody [EPR6436(2)] (AB154843)

Lane 1 : Wild-type HAP1 cell lysate (20 μg)
Lane 2 : ATG4B knockout HAP1 cell lysate (20 μg)

Lanes 1 - 2 : Merged signal (red and green). Green - ab154843 observed at 47 kDa. Red - loading control, ab18058, observed at 130 kDa.

ab154843 was shown to specifically react with ATG4B when ATG4B knockout samples were used. Wild-type and ATG4B knockout samples were subjected to SDS-PAGE. ab154843 and ab18058 (loading control to Vinculin) were diluted at 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ATG4B antibody [EPR6436(2)] (ab154843)

Predicted band size: 44 kDa

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Western blot - Anti-ATG4B antibody [EPR6436(2)] (AB154843)
  • WB

Lab

Western blot - Anti-ATG4B antibody [EPR6436(2)] (AB154843)

Lanes 1 - 2 : Merged signal (red and green). Green - ab154843 observed at 47 kDa. Red - loading control ab8245 (Mouse anti-GAPDH antibody [6C5]) observed at 37kDa.
ab154843 was shown to react with ATG4B in wild-type HeLa cells in Western blot with loss of signal observed in ATG4B knockout cell line ab260973 (ATG4B knockout cell lysate ab257364). Wild-type and ATG4B knockout HEK293T cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3 % milk in TBS-T (0.1 % Tween®) before incubation with ab154843 and ab8245 (Mouse anti-GAPDH antibody [6C5]) overnight at 4 °C at a 1 in 1000 dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 h at room temperature before imaging.

All lanes:

Western blot - Anti-ATG4B antibody [EPR6436(2)] (ab154843) at 1/1000 dilution

Lane 1:

Wild-type HeLa cell lysate at 20 µg

Lane 2:

ATG4B knockout HeLa cell lysate at 20 µg

Lane 2:

Western blot - Human ATG4B knockout HeLa cell line (<a href='/en-us/products/cell-lines/human-atg4b-knockout-hela-cell-line-ab265814'>ab265814</a>)

Predicted band size: 44 kDa

Observed band size: 47 kDa

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Western blot - Anti-ATG4B antibody [EPR6436(2)] (AB154843)
  • WB

Unknown

Western blot - Anti-ATG4B antibody [EPR6436(2)] (AB154843)

All lanes:

Western blot - Anti-ATG4B antibody [EPR6436(2)] (ab154843) at 1/1000 dilution

Lane 1:

Ramos cell lysate at 10 µg

Lane 2:

293T cell lysate at 10 µg

Lane 3:

Jurkat cell lysate at 10 µg

Lane 4:

HeLa cell lysate at 10 µg

Predicted band size: 44 kDa

false

  • Carrier free

    Anti-ATG4B antibody [EPR6436(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6436(2)

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Human

Applications

Flow Cyt (Intra), WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purity
Tissue culture supernatant
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 50% Tissue culture supernatant, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATG4B is a cysteine protease also known as autophagin-1 with a molecular mass of approximately 45 kDa. It is involved in the autophagy process by cleaving ATG8 family proteins. This protease facilitates the lipidation of LC3 an essential component of the autophagosome membrane. You find ATG4B expressed in various tissues especially in the brain liver and muscle where it plays important roles in cellular maintenance.
Biological function summary

ATG4B functions in the cellular autophagic machinery cleaving the C-terminal of ATG8 proteins. This processing is necessary for the conjugation to phosphatidylethanolamine and integration into autophagosomes. ATG4B is part of a larger autophagic complex that also includes other critical proteins like ATG7 and ATG3 helping prepare ATG8 proteins for autophagosome creation.

Pathways

ATG4B participates in the autophagy pathway by refining the cargo processing mechanism. It interacts with the mTOR signaling pathway which influences cell survival and growth based on nutrient availability. The ATG4B enzyme is closely related to ATG7 in the pathway as both contribute to the maturation of autophagosomes.

ATG4B has significant links to cancer and neurodegenerative disorders. In cancer ATG4B activity affects tumor survival since cancer cells exploit autophagy for growth under stress conditions. In neurodegenerative diseases like Alzheimer's alterations in ATG4B activity disrupt cellular homeostasis. This disruption connects to proteins like LC3 which are pivotal in neuron survival and function.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins (PubMed : 15169837, PubMed : 15187094, PubMed : 17347651, PubMed : 19322194, PubMed : 21177865, PubMed : 22302004, PubMed : 26378241, PubMed : 27527864, PubMed : 28633005, PubMed : 28821708, PubMed : 29232556, PubMed : 30076329, PubMed : 30443548, PubMed : 30661429). Required for canonical autophagy (macroautophagy), non-canonical autophagy as well as for mitophagy (PubMed : 33773106, PubMed : 33909989). The protease activity is required for proteolytic activation of ATG8 family proteins : cleaves the C-terminal amino acid of ATG8 proteins MAP1LC3A, MAP1LC3B, MAP1LC3C, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine (PubMed : 15169837, PubMed : 15187094, PubMed : 17347651, PubMed : 19322194, PubMed : 20818167, PubMed : 21177865, PubMed : 22302004, PubMed : 27527864, PubMed : 28287329, PubMed : 28633005, PubMed : 29458288, PubMed : 30661429). Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy (PubMed : 15169837, PubMed : 15187094, PubMed : 17347651, PubMed : 19322194, PubMed : 21177865, PubMed : 22302004). Protease activity is also required to counteract formation of high-molecular weight conjugates of ATG8 proteins (ATG8ylation) : acts as a deubiquitinating-like enzyme that removes ATG8 conjugated to other proteins, such as ATG3 (PubMed : 31315929, PubMed : 33773106). In addition to the protease activity, also mediates delipidation of ATG8 family proteins (PubMed : 15187094, PubMed : 19322194, PubMed : 28633005, PubMed : 29458288, PubMed : 32686895, PubMed : 33909989). Catalyzes delipidation of PE-conjugated forms of ATG8 proteins during macroautophagy (PubMed : 15187094, PubMed : 19322194, PubMed : 29458288, PubMed : 32686895, PubMed : 33909989). Also involved in non-canonical autophagy, a parallel pathway involving conjugation of ATG8 proteins to single membranes at endolysosomal compartments, by catalyzing delipidation of ATG8 proteins conjugated to phosphatidylserine (PS) (PubMed : 33909989). Compared to other members of the family (ATG4A, ATG4C or ATG4C), constitutes the major protein for proteolytic activation of ATG8 proteins, while it displays weaker delipidation activity than other ATG4 paralogs (PubMed : 29458288, PubMed : 30661429). Involved in phagophore growth during mitophagy independently of its protease activity and of ATG8 proteins : acts by regulating ATG9A trafficking to mitochondria and promoting phagophore-endoplasmic reticulum contacts during the lipid transfer phase of mitophagy (PubMed : 33773106).
See full target information ATG4B
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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

BMB reports 57:497-502 PubMed39384175

2024

Differential roles of N- and C-terminal LIR motifs in the catalytic activity and membrane targeting of RavZ and ATG4B proteins.

Applications

Unspecified application

Species

Unspecified reactive species

Sang-Won Park,Ju-Hui Park,Haneul Choi,Pureum Jeon,Seung-Hwan Lee,Won-Dong Shin,Hun-Joo Kim,Jin-A Lee,Deok-Jin Jang

Lipids in health and disease 23:308 PubMed39334359

2024

Empagliflozin alleviates obesity-related cardiac dysfunction via the activation of SIRT3-mediated autophagosome formation.

Applications

Unspecified application

Species

Unspecified reactive species

Youhong Luo,Tongtong Ye,Hongzhan Tian,Hongwei Song,Chengxia Kan,Fang Han,Ningning Hou,Xiaodong Sun,Jingwen Zhang

Heliyon 9:e22467 PubMed38074857

2023

LncRNA AC125982.2 regulates apoptosis of cardiomyocytes through mir-450b-3p/ATG4B axis in a rat model with myocardial infarction.

Applications

Unspecified application

Species

Unspecified reactive species

Zhi-Jun Xu,Peng-Yin Zhang,Zhen-Qiu Li,Han-Ping Zhu,Wei-Lu Tan,Pei-Hua Ren

International journal of molecular sciences 24: PubMed37239971

2023

The miRNA Content of Bone Marrow-Derived Extracellular Vesicles Contributes to Protein Pathway Alterations Involved in Ionising Radiation-Induced Bystander Responses.

Applications

Unspecified application

Species

Unspecified reactive species

Ilona Barbara Csordás,Eric Andreas Rutten,Tünde Szatmári,Prabal Subedi,Lourdes Cruz-Garcia,Dávid Kis,Bálint Jezsó,Christine von Toerne,Martina Forgács,Géza Sáfrány,Soile Tapio,Christophe Badie,Katalin Lumniczky

International journal of oncology 62: PubMed36825592

2023

Dual targets of lethal apoptosis and protective autophagy in liver cancer with periplocymarin elicit a limited therapeutic effect.

Applications

Unspecified application

Species

Unspecified reactive species

Yuanyuan Hao,Tao Song,Mingye Wang,Tongtong Li,Chi Zhao,Ting Li,Yunlong Hou,Hongjiang He

Neoplasma 70:114-122 PubMed36704921

2023

Cryptotanshinone inhibits oral squamous cell carcinoma through the autophagic pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao-Tian Jiang,Ye Qiu,Cong-Hua Li

Cells 11: PubMed35740992

2022

BECLIN-1-Mediated Autophagy Suppresses Silica Nanoparticle-Induced Testicular Toxicity via the Inhibition of Caspase 8-Mediated Cell Apoptosis in Leydig Cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qianru Zhang,Jason William Grunberger,Nitish Khurana,Xin Zhou,Xianyu Xu,Hamidreza Ghandehari,Fenglei Chen

Human molecular genetics 29:3589-3605 PubMed33372681

2020

Mitochondria-lysosome membrane contacts are defective in GDAP1-related Charcot-Marie-Tooth disease.

Applications

Unspecified application

Species

Unspecified reactive species

Lara Cantarero,Elena Juárez-Escoto,Azahara Civera-Tregón,María Rodríguez-Sanz,Mónica Roldán,Raúl Benítez,Janet Hoenicka,Francesc Palau

Investigative ophthalmology & visual science 61:19 PubMed32926102

2020

S100A4 Silencing Facilitates Corneal Wound Healing After Alkali Burns by Promoting Autophagy via Blocking the PI3K/Akt/mTOR Signaling Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yulin Wang,Guiping Gao,Ying Wu,Yuqin Wang,Xiaorong Wu,Qiong Zhou

Cell death & disease 9:483 PubMed29706629

2018

Targeting the miR-665-3p-ATG4B-autophagy axis relieves inflammation and apoptosis in intestinal ischemia/reperfusion.

Applications

Unspecified application

Species

Unspecified reactive species

Zhenlu Li,Guangzhi Wang,Dongcheng Feng,Guo Zu,Yang Li,Xue Shi,Yan Zhao,Huirong Jing,Shili Ning,Weidong Le,Jihong Yao,Xiaofeng Tian
View all publications
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