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Rabbit Recombinant Monoclonal ATG4C antibody. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 3 publications.

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Images

Western blot - Anti-ATG4C antibody [EPR15001] (AB183516), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4C antibody [EPR15001] (AB183516), expandable thumbnail
  • Western blot - Anti-ATG4C antibody [EPR15001] (AB183516), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-ATG4C antibody [EPR15001] (AB183516), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
WBFlow Cyt (Intra)IHC-P
Human
Tested
Tested
Tested

Tested
Tested

Species
Human
Dilution info
1/1000
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Tested
Tested

Species
Human
Dilution info
1/100
Notes

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

Tested
Tested

Species
Human
Dilution info
1/250
Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target data

Function

Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins (PubMed:21177865, PubMed:29458288, PubMed:30661429). The protease activity is required for proteolytic activation of ATG8 family proteins: cleaves the C-terminal amino acid of ATG8 proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine (PubMed:21177865). Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy (By similarity). In addition to the protease activity, also mediates delipidation of ATG8 family proteins (PubMed:29458288, PubMed:33909989). Catalyzes delipidation of PE-conjugated forms of ATG8 proteins during macroautophagy (PubMed:29458288, PubMed:33909989). Compared to ATG4B, the major protein for proteolytic activation of ATG8 proteins, shows weaker ability to cleave the C-terminal amino acid of ATG8 proteins, while it displays stronger delipidation activity (PubMed:29458288). In contrast to other members of the family, weakly or not involved in phagophore growth during mitophagy (PubMed:33773106).

Alternative names

APG4C, AUTL1, AUTL3, ATG4C, Cysteine protease ATG4C, AUT-like 3 cysteine endopeptidase, Autophagy-related cysteine endopeptidase 3, Autophagy-related protein 4 homolog C, Autophagin-3, HsAPG4C

Recommended products

Rabbit Recombinant Monoclonal ATG4C antibody. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 3 publications.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR15001
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ATG4C also known as autophagin-3 is a cysteine protease involved in autophagy an important cellular degradation pathway. With a molecular mass of approximately 52 kDa the target plays a significant role in cytosolic processing. Primarily ATG4C acts in various tissues including the brain liver and muscle where it participates in the processing of essential autophagy-related proteins. The protein provides an enzymatic function necessary for the maturation and recycling of autophagosomes structures required for autophagy.

Biological function summary

ATG4C cleaves the ATG8 family proteins such as LC3 at a conserved glycine residue facilitating their conjugation to phosphatidylethanolamine. This step is critical in the elongation of the isolation membrane during autophagosome formation. ATG4C is not typically found as part of a large protein complex but its interaction with ATG8 family members is an important regulatory point. Its function in recycling ATG8-family proteins helps maintain cellular homeostasis and responds to stress conditions like nutrient deprivation.

Pathways

ATG4C plays a central role in the autophagy pathway essential for cell survival differentiation and tissue remodeling. The protein closely interacts with components of the autophagy machinery such as ATG5 and the ULK1 complex coordinating the effective sequestration of cytoplasmic constituents. Its activity impacts the broader cellular pathways involving mTOR and AMPK both of which regulate cellular energy status and respond to metabolic signals positioning ATG4C as a vital component in these pathways.

Associated diseases and disorders

Alterations in ATG4C function have been linked to cancer and neurodegenerative disorders. In cancer dysregulated autophagy affects tumor growth and therapy resistance. ATG4C is related to Beclin 1 which regulates autophagy initiation in tumors. In neurodegeneration impaired autophagy exacerbates the accumulation of protein aggregates. Misfunction of ATG4C can influence these processes showing a direct connection to p62/SQSTM1 a protein involved in defective degradation that leads to neurodegenerative pathology.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-ATG4C antibody [EPR15001] (ab183516), expandable thumbnail

    Western blot - Anti-ATG4C antibody [EPR15001] (ab183516)

    Lanes 1-4: Merged signal (red and green). Green - ab183516 observed at 52 kDa. Red - loading control Anti-GAPDH antibody [6C5] - Loading Control ab8245 observed at 36 kDa.

    ab183516 Anti-ATG4C antibody [EPR15001] was shown to specifically react with ATG4C in wild-type HEK293T cells. Loss of signal was observed when knockout cell line Human ATG4C knockout HEK-293T cell line ab267324 (knockout cell lysate Human ATG4C knockout HEK-293T cell lysate ab257847) was used. Wild-type and ATG4C knockout samples were subjected to SDS-PAGE. ab183516 and Anti-GAPDH antibody [6C5] - Loading Control (Anti-GAPDH antibody [6C5] - Loading Control ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-ATG4C antibody [EPR15001] (ab183516) at 1/1000 dilution

    Lane 1: Wild-type HEK293T cell lysate at 20 µg

    Lane 2: ATG4C knockout HEK293T cell lysate at 20 µg

    Lane 2: Western blot - Human ATG4C knockout HEK-293T cell line (Human ATG4C knockout HEK-293T cell line ab267324)

    Lane 3: Jurkat cell lysate at 20 µg

    Lane 4: Raji cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) at 1/10000 dilution

    Predicted band size: 52 kDa

    Observed band size: 52 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4C antibody [EPR15001] (ab183516), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4C antibody [EPR15001] (ab183516)

    Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling ATG4C with ab183516 at 1/250 dilution. The slide is counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

  • Western blot - Anti-ATG4C antibody [EPR15001] (ab183516), expandable thumbnail

    Western blot - Anti-ATG4C antibody [EPR15001] (ab183516)

    All lanes: Western blot - Anti-ATG4C antibody [EPR15001] (ab183516) at 1/20000 dilution

    Lane 1: Jurkat cell lysate at 20 µg

    Lane 2: Raji cell lysate at 20 µg

    Lane 3: 293 cell lysate at 20 µg

    Secondary

    All lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 52 kDa

  • Flow Cytometry (Intracellular) - Anti-ATG4C antibody [EPR15001] (ab183516), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ATG4C antibody [EPR15001] (ab183516)

    Intracellular flow cytometric analysis of 2%paraformaldehyde-fixed 293 cells labeling ATG4Cwith ab183516 at 1/100 dilution (green). Goat anti-rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control (red).

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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