AB183516

Anti-ATG4C antibody [EPR15001]

RabMAb
Recombinant
KO Validated
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(3 Publications)

Rabbit Recombinant Monoclonal ATG4C antibody. Suitable for WB, Flow Cyt (Intra), IHC-P and reacts with Human samples. Cited in 3 publications.

View Alternative Names

APG4C, AUTL1, AUTL3, ATG4C, Cysteine protease ATG4C, AUT-like 3 cysteine endopeptidase, Autophagy-related cysteine endopeptidase 3, Autophagy-related protein 4 homolog C, Autophagin-3, HsAPG4C

4 Images

Flow Cytometry (Intracellular) - Anti-ATG4C antibody [EPR15001] (AB183516)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ATG4C antibody [EPR15001] (AB183516)

Intracellular flow cytometric analysis of 2%paraformaldehyde-fixed 293 cells labeling ATG4Cwith ab183516 at 1/100 dilution (green). Goat anti-rabbit IgG (FITC) at 1/150 dilution was used as the secondary antibody. Rabbit monoclonal IgG was used as the isotype control (red).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4C antibody [EPR15001] (AB183516)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4C antibody [EPR15001] (AB183516)

Immunohistochemical analysis of paraffin-embedded Human skeletal muscle tissue labeling ATG4C with ab183516 at 1/250 dilution. The slide is counterstained with Hematoxylin.

Perform heat mediated antigen retrieval with EDTA buffer pH 9 before commencing with IHC staining protocol.

Lab

Western blot - Anti-ATG4C antibody [EPR15001] (AB183516)

Lanes 1-4 : Merged signal (red and green). Green - ab183516 observed at 52 kDa. Red - loading control ab8245 observed at 36 kDa.

ab183516 Anti-ATG4C antibody [EPR15001] was shown to specifically react with ATG4C in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab267324 (knockout cell lysate ab257847) was used. Wild-type and ATG4C knockout samples were subjected to SDS-PAGE. ab183516 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

All lanes:

Western blot - Anti-ATG4C antibody [EPR15001] (ab183516) at 1/1000 dilution

Lane 1:

Wild-type HEK293T cell lysate at 20 µg

Lane 2:

ATG4C knockout HEK293T cell lysate at 20 µg

Lane 2:

Western blot - Human ATG4C knockout HEK-293T cell line (<a href='/en-us/products/cell-lines/human-atg4c-knockout-hek-293t-cell-line-ab267324'>ab267324</a>)

Lane 3:

Jurkat cell lysate at 20 µg

Lane 4:

Raji cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-irdye-800cw-preadsorbed-ab216773'>ab216773</a>) at 1/10000 dilution

Predicted band size: 52 kDa

Observed band size: 52 kDa

false

Supplier Data

Western blot - Anti-ATG4C antibody [EPR15001] (AB183516)

All lanes:

Western blot - Anti-ATG4C antibody [EPR15001] (ab183516) at 1/20000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

Raji cell lysate at 20 µg

Lane 3:

293 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 52 kDa

false

Carrier free

Anti-ATG4C antibody [EPR15001] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR15001

Isotype

IgG

Carrier free

Reacts with

Human

Applications

Flow Cyt (Intra), WB, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/100", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/250", "IHCP-species-notes": "" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATG4C also known as autophagin-3 is a cysteine protease involved in autophagy an important cellular degradation pathway. With a molecular mass of approximately 52 kDa the target plays a significant role in cytosolic processing. Primarily ATG4C acts in various tissues including the brain liver and muscle where it participates in the processing of essential autophagy-related proteins. The protein provides an enzymatic function necessary for the maturation and recycling of autophagosomes structures required for autophagy.

Biological function summary

ATG4C cleaves the ATG8 family proteins such as LC3 at a conserved glycine residue facilitating their conjugation to phosphatidylethanolamine. This step is critical in the elongation of the isolation membrane during autophagosome formation. ATG4C is not typically found as part of a large protein complex but its interaction with ATG8 family members is an important regulatory point. Its function in recycling ATG8-family proteins helps maintain cellular homeostasis and responds to stress conditions like nutrient deprivation.

Pathways

ATG4C plays a central role in the autophagy pathway essential for cell survival differentiation and tissue remodeling. The protein closely interacts with components of the autophagy machinery such as ATG5 and the ULK1 complex coordinating the effective sequestration of cytoplasmic constituents. Its activity impacts the broader cellular pathways involving mTOR and AMPK both of which regulate cellular energy status and respond to metabolic signals positioning ATG4C as a vital component in these pathways.

Alterations in ATG4C function have been linked to cancer and neurodegenerative disorders. In cancer dysregulated autophagy affects tumor growth and therapy resistance. ATG4C is related to Beclin 1 which regulates autophagy initiation in tumors. In neurodegeneration impaired autophagy exacerbates the accumulation of protein aggregates. Misfunction of ATG4C can influence these processes showing a direct connection to p62/SQSTM1 a protein involved in defective degradation that leads to neurodegenerative pathology.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Cysteine protease that plays a key role in autophagy by mediating both proteolytic activation and delipidation of ATG8 family proteins (PubMed : 21177865, PubMed : 29458288, PubMed : 30661429). The protease activity is required for proteolytic activation of ATG8 family proteins : cleaves the C-terminal amino acid of ATG8 proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine (PubMed : 21177865). Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy (By similarity). In addition to the protease activity, also mediates delipidation of ATG8 family proteins (PubMed : 29458288, PubMed : 33909989). Catalyzes delipidation of PE-conjugated forms of ATG8 proteins during macroautophagy (PubMed : 29458288, PubMed : 33909989). Compared to ATG4B, the major protein for proteolytic activation of ATG8 proteins, shows weaker ability to cleave the C-terminal amino acid of ATG8 proteins, while it displays stronger delipidation activity (PubMed : 29458288). In contrast to other members of the family, weakly or not involved in phagophore growth during mitophagy (PubMed : 33773106).

See full target information ATG4C

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 24: PubMed38003226

2023

WISP1 Is Involved in the Pathogenesis of Kashin-Beck Disease via the Autophagy Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Ping Li,Bolun Cheng,Yao Yao,Wenxing Yu,Li Liu,Shiqiang Cheng,Lu Zhang,Mei Ma,Xin Qi,Chujun Liang,Xiaomeng Chu,Jing Ye,Shiquan Sun,Yumeng Jia,Xiong Guo,Yan Wen,Feng Zhang

Science advances 8:eabo0412 PubMed35921421

2022

Deacetylation of ATG4B promotes autophagy initiation under starvation.

Applications

Unspecified application

Species

Unspecified reactive species

Liangbo Sun,Haojun Xiong,Lingxi Chen,Xufang Dai,Xiaojing Yan,Yaran Wu,Mingzhen Yang,Meihua Shan,Tao Li,Jie Yao,Wenbin Jiang,Haiyan He,Fengtian He,Jiqin Lian

Autophagy 14:992-1010 PubMed29458288

2018

Delipidation of mammalian Atg8-family proteins by each of the four ATG4 proteases.

Applications

Unspecified application

Species

Unspecified reactive species

Karlina J Kauffman,Shenliang Yu,Jiaxin Jin,Brian Mugo,Nathan Nguyen,Aidan O'Brien,Shanta Nag,Alf Håkon Lystad,Thomas J Melia

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATG4C antibody [EPR15001]

Flow Cytometry (Intracellular) - Anti-ATG4C antibody [EPR15001] (AB183516)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG4C antibody [EPR15001] (AB183516)

Western blot - Anti-ATG4C antibody [EPR15001] (AB183516)

Western blot - Anti-ATG4C antibody [EPR15001] (AB183516)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise