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AB223380

Anti-ATG7 antibody

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(4 Publications )

Rabbit Polyclonal ATG7 antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Cited in 4 publications. Immunogen corresponding to Synthetic Peptide within Human ATG7.

View Alternative Names

APG7L, ATG7, Ubiquitin-like modifier-activating enzyme ATG7, ATG12-activating enzyme E1 ATG7, Autophagy-related protein 7, Ubiquitin-activating enzyme E1-like protein, APG7-like, hAGP7

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-ATG7 antibody (AB223380)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ATG7 antibody (AB223380)

4% formaldehyde-fixed RKO cells stained for ATG7 (green) using ab223380 at 1/100 dilution in ICC/IF. Secondary Antibody : Goat Anti-Rabbit ATTO 488 at 1/100 for 60 min at RT. Counterstain : Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1/1000, 1/5000 for 60 min at RT, 5 min at RT.

(A) DAPI nuclear stain (B) Phalloidin Texas Red F-Actin stain (C) ab223380 (D) Merge.

Western blot - Anti-ATG7 antibody (AB223380)
  • WB

Supplier Data

Western blot - Anti-ATG7 antibody (AB223380)

Blocking buffer : 5% skim milk in TBST.

Primary and secondary antibodies were incubated for 1 hour at RT.

All lanes:

Western blot - Anti-ATG7 antibody (ab223380) at 1/1000 dilution

All lanes:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP at 1/2000 dilution

Predicted band size: 77 kDa

Observed band size: 78 kDa

true

Exposure time: 6min

Western blot - Anti-ATG7 antibody (AB223380)
  • WB

Supplier Data

Western blot - Anti-ATG7 antibody (AB223380)

Blocking buffer : 2% BSA and 2% skim milk in TBST.

Primary antibody was incubated for 16 hours at 4°C, the secondary for 1 hour at RT.

All lanes:

Western blot - Anti-ATG7 antibody (ab223380) at 1/1000 dilution

All lanes:

Rat brain cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG HRP at 1/2000 dilution

Predicted band size: 77 kDa

Observed band size: 78 kDa

true

Exposure time: 6min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

ICC/IF, WB

applications

Immunogen

Synthetic Peptide within Human ATG7. The exact immunogen used to generate this antibody is proprietary information.

O95352

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATG7 also known as Autophagy Related 7 is an essential protein involved in the autophagy process. It functions as an E1-like enzyme activating and transferring ubiquitin-like proteins such as ATG12 and LC3. The molecular weight of ATG7 is approximately 78 kDa. It is widely expressed in various tissues although expression levels can differ. One can detect ATG7 using immunoassays like ELISA or antibodies specifically targeting ATG7. Understanding its mechanical role is key to studying cellular homeostasis.
Biological function summary

ATG7 plays a significant role in autophagy a cellular degradation pathway critical for cell survival under stress. ATG7 contributes to the formation of autophagosomes by facilitating conjugation of ATG8 family proteins including LC3 to phosphatidylethanolamine. It acts within complexes that regulate cellular energy balance and stress responses ensuring cells maintain their function and integrity. Knockdown of ATG7 can impair autophagic flux highlighting its importance in maintaining cellular processes.

Pathways

ATG7 is a central player in the autophagy pathway influencing cellular metabolism and turnover. It interacts closely with ATG5 and ATG12 in this pathway to form a conjugation system essential for autophagosome elongation. Additionally ATG7 is involved in the mTOR signaling pathway which regulates nutrient sensing and cellular growth. Interaction with proteins like mTOR allows ATG7 to integrate signals from nutrient availability and stress responses finely tuning the autophagy process.

ATG7 dysfunction has connections to cancer and neurodegenerative diseases like Alzheimer's. Abnormal ATG7 activity disrupts autophagic balance possibly leading to the accumulation of damaged proteins and organelles contributing to disease progression. In cancer altered ATG7 expression may influence tumor survival by affecting cellular stress responses. Proteins such as p53 involved in cell cycle regulation often show association with ATG7-related pathways indicating a complex network influencing disease states. Understanding ATG7's role in these conditions can help explore potential therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 as well as the ATG8 family proteins for their conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes membranes. Required for autophagic death induced by caspase-8 inhibition. Facilitates LC3-I lipidation with phosphatidylethanolamine to form LC3-II which is found on autophagosomal membranes (PubMed : 34161705). Required for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Modulates p53/TP53 activity to regulate cell cycle and survival during metabolic stress. Also plays a key role in the maintenance of axonal homeostasis, the prevention of axonal degeneration, the maintenance of hematopoietic stem cells, the formation of Paneth cell granules, as well as in adipose differentiation. Plays a role in regulating the liver clock and glucose metabolism by mediating the autophagic degradation of CRY1 (clock repressor) in a time-dependent manner (By similarity).
See full target information ATG7
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Publications (4)

Recent publications for all applications. Explore the full list and refine your search

Translational oncology 46:101996 PubMed38795560

2024

Regulatory feedback loop between circ-EIF4A3 and EIF4A3 Enhances autophagy and growth in colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Qingke Li,Zhiwu Wang,Jian Wang,Jiangong Wang,Xuan Zheng,Dan Li,Zhuo Wang,Jingwu Li,Yufeng Li

Microbiology spectrum 10:e0071822 PubMed35768946

2022

-Induced Mitophagy That Balances Mitochondrial Homeostasis and mROS-Mediated Bactericidal Activity.

Applications

Unspecified application

Species

Unspecified reactive species

Yang Jiao,Shiyang Cao,Yuan Zhang,Yafang Tan,Yazhou Zhou,Tong Wang,Yang You,Hongyan Chen,Yifan Ren,Ruifu Yang,Zongmin Du

Molecular neurobiology 59:1199-1213 PubMed34962635

2021

Autophagy Inhibition Reduces Irradiation-Induced Subcortical White Matter Injury Not by Reducing Inflammation, but by Increasing Mitochondrial Fusion and Inhibiting Mitochondrial Fission.

Applications

Unspecified application

Species

Unspecified reactive species

Yafeng Wang,Yiran Xu,Kai Zhou,Shan Zhang,Yong Wang,Tao Li,Cuicui Xie,Xiaoli Zhang,Juan Song,Xiaoyang Wang,Changlian Zhu

Oxidative medicine and cellular longevity 2020:5954635 PubMed33488926

2020

Melatonin Protects Goat Spermatogonial Stem Cells against Oxidative Damage during Cryopreservation by Improving Antioxidant Capacity and Inhibiting Mitochondrial Apoptosis Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Tian-Yu Feng,Qian Li,Fa Ren,Hua-Ming Xi,Dong-Liang Lv,Yu Li,Jian-Hong Hu
View all publications
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Product promise

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