AB99001

Anti-ATG7 antibody

Name: Anti-ATG7 antibody (ab99001) | Abcam
Brand: Abcam Limited
SKU: AB99001

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(2 Publications)

Rabbit Polyclonal ATG7 antibody. Suitable for WB and reacts with Arabidopsis thaliana samples. Cited in 2 publications. Immunogen corresponding to Full Length Protein corresponding to Arabidopsis thaliana ATG7.

View Alternative Names

APG7, PEUP4, At5g45900, K15I22.10, ATG7, Ubiquitin-like modifier-activating enzyme atg7, ATG12-activating enzyme E1 atg7, Autophagy-related protein 7, Protein PEROXISOME UNUSUAL POSITIONING 4, AtAPG7

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Arabidopsis thaliana

Applications

applications

Immunogen

Full Length Protein corresponding to Arabidopsis thaliana ATG7. The exact immunogen used to generate this antibody is proprietary information.

Q94CD5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Arabidopsis thaliana": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purity

Whole antiserum

Storage buffer

Constituents: Whole serum

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATG7 also known as Autophagy Related 7 is an essential protein involved in the autophagy process. It functions as an E1-like enzyme activating and transferring ubiquitin-like proteins such as ATG12 and LC3. The molecular weight of ATG7 is approximately 78 kDa. It is widely expressed in various tissues although expression levels can differ. One can detect ATG7 using immunoassays like ELISA or antibodies specifically targeting ATG7. Understanding its mechanical role is key to studying cellular homeostasis.

Biological function summary

ATG7 plays a significant role in autophagy a cellular degradation pathway critical for cell survival under stress. ATG7 contributes to the formation of autophagosomes by facilitating conjugation of ATG8 family proteins including LC3 to phosphatidylethanolamine. It acts within complexes that regulate cellular energy balance and stress responses ensuring cells maintain their function and integrity. Knockdown of ATG7 can impair autophagic flux highlighting its importance in maintaining cellular processes.

Pathways

ATG7 is a central player in the autophagy pathway influencing cellular metabolism and turnover. It interacts closely with ATG5 and ATG12 in this pathway to form a conjugation system essential for autophagosome elongation. Additionally ATG7 is involved in the mTOR signaling pathway which regulates nutrient sensing and cellular growth. Interaction with proteins like mTOR allows ATG7 to integrate signals from nutrient availability and stress responses finely tuning the autophagy process.

ATG7 dysfunction has connections to cancer and neurodegenerative diseases like Alzheimer's. Abnormal ATG7 activity disrupts autophagic balance possibly leading to the accumulation of damaged proteins and organelles contributing to disease progression. In cancer altered ATG7 expression may influence tumor survival by affecting cellular stress responses. Proteins such as p53 involved in cell cycle regulation often show association with ATG7-related pathways indicating a complex network influencing disease states. Understanding ATG7's role in these conditions can help explore potential therapeutic strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

E1-like activating enzyme involved in the 2 ubiquitin-like systems required for cytoplasm to vacuole transport (Cvt) and autophagy. Activates ATG12 for its conjugation with ATG5 and ATG8 for its conjugation with phosphatidylethanolamine. Both systems are needed for the ATG8 association to Cvt vesicles and autophagosomes. Involved in the senescence process (PubMed : 12070171). Involved in the degradation of damaged peroxisomes (PubMed : 24368788). Involved in the non-selective degradation of chlorophylls and photosynthetic proteins during stress-induced leaf yellowing (PubMed : 24510943).

See full target information ATG7

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

The Plant cell 32:263-284 PubMed31732704

2019

Arabidopsis SINAT Proteins Control Autophagy by Mediating Ubiquitylation and Degradation of ATG13.

Applications

Unspecified application

Species

Unspecified reactive species

Hua Qi,Juan Li,Fan-Nv Xia,Jin-Yu Chen,Xue Lei,Mu-Qian Han,Li-Juan Xie,Qing-Ming Zhou,Shi Xiao

Frontiers in plant science 8:1229 PubMed28747924

2017

Endocytosis of AtRGS1 Is Regulated by the Autophagy Pathway after D-Glucose Stimulation.

Applications

Unspecified application

Species

Unspecified reactive species

Quanquan Yan,Jingchun Wang,Zheng Qing Fu,Wenli Chen

View all publications

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Product promise

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For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ATG7 antibody

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

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