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Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Rabbit Polyclonal ATG8 antibody. Suitable for ELISA, WB and reacts with Saccharomyces cerevisiae samples. Cited in 10 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C ATG8.
IgG
Rabbit
pH: 6.5
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Tripotassium orthophosphate
Liquid
Polyclonal
ELISA | WB | |
---|---|---|
Saccharomyces cerevisiae | Expected | Tested |
Species | Dilution info | Notes |
---|---|---|
Species Saccharomyces cerevisiae | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
---|---|---|
Species Saccharomyces cerevisiae | Dilution info - | Notes This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates). Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions. |
Ubiquitin-like modifier involved in cytoplasm to vacuole transport (Cvt) vesicles and autophagosomes formation. With ATG4, mediates the delivery of the vesicles and autophagosomes to the vacuole via the microtubule cytoskeleton. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Participates also in membrane fusion events that take place in the early secretory pathway. Also involved in endoplasmic reticulum-specific autophagic process and is essential for the survival of cells subjected to severe ER stress. The ATG8-PE conjugate mediates tethering between adjacent membranes and stimulates membrane hemifusion, leading to expansion of the autophagosomal membrane during autophagy. Moreover not only conjugation, but also subsequent ATG8-PE deconjugation is an important step required to facilitate multiple events during macroautophagy, and especially for efficient autophagosome biogenesis, the assembly of ATG9-containing tubulovesicular clusters into phagophores/autophagosomes, and for the disassembly of PAS-associated ATG components. Plays also a role in regulation of filamentous growth.
Autophagy-related protein 8, Autophagy-related ubiquitin-like modifier ATG8, Cytoplasm to vacuole targeting protein 5, AUT7, ATG8, APG8, YBL0732, CVT5, YBL078C
Rabbit Polyclonal ATG8 antibody. Suitable for ELISA, WB and reacts with Saccharomyces cerevisiae samples. Cited in 10 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C ATG8.
Autophagy-related protein 8, Autophagy-related ubiquitin-like modifier ATG8, Cytoplasm to vacuole targeting protein 5, AUT7, ATG8, APG8, YBL0732, CVT5, YBL078C
IgG
Rabbit
pH: 6.5
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Tripotassium orthophosphate
Liquid
Polyclonal
Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C ATG8. Database link P38182
Ion exchange chromatography
Anti-Apg8 antibody, also known as Atg8, is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
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The ATG8 protein assists in the selective degradation of damaged organelles and proteins through autophagy. It is a member of the ATG ubiquitin-like protein family and works in collaboration with other autophagy proteins such as ATG3 and ATG7. These interactions form complexes necessary for the autophagic process. ATG8 acts as a marker for autophagosomes guiding cargo to be engulfed and recycled effectively.
The ATG8 protein also known as autophagy-related protein 8 weighs approximately 14 kDa and plays an important role in autophagy. It is expressed widely in eukaryotic cells including yeast plants and mammals. ATG8 engages in the conjugation with phospholipid phosphatidylethanolamine (PE) on the surface of autophagic vesicles. This conjugation allows the vesicles to elongate and form autophagosomes which are important for the engulfment of cellular components that need to be degraded.
ATG8 functions within the autophagy pathway and the lysosome degradation pathway which are essential for cellular maintenance and nutrient recycling. In these pathways ATG8 interacts with proteins like mTOR and ULK1 that regulate autophagy. As a central player it connects upstream signaling events with downstream autophagic membrane dynamics facilitating cellular adaptation in response to nutrient availability and stress conditions.
Alterations in ATG8 function relate to neurodegenerative diseases such as Parkinson's disease and Huntington's disease. Dysregulation of autophagy where ATG8 plays a critical role impacts protein aggregation and cellular toxicity commonly seen in these conditions. Additionally it connects with proteins like p62 in these disorders as impaired protein clearance pathways influence disease progression. ATG8 involvement highlights its significance in maintaining neuronal health and potentially in therapeutic strategies targeting aberrant autophagic processes.
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We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
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Terms & Conditions.
Ab4753, generated by immunization with recombinant yeast ATG8 (or Apg 8), was tested by immunoblot with other anti-UBL (Ubiquitin-like modifier) antibodies against E.coli lysates expressing the ATG8-GFP fusion protein (Apg 8-GFP in both panels)). All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL.
Panel A shows total protein staining using ponceau.
Panel B shows specific reaction with ATG8 (Apg 8) using a 1:4,000 and 1:8,000 dilution of ab4753 followed by reaction with a 1:15,000 dilution of HRP Goat-a-Rabbit IgG (ab7090). All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4° C. E.coli lysate proteins were separated by SDS-PAGE using a 15% gel.
Similar experiments (data not shown), where other UBL fusion proteins were separated and probed with this antibody showed no react
All lanes: Western blot - Anti-ATG8 antibody (AB4753)
Predicted band size: 14 kDa
Gel run under denaturing conditions.
Primary antibody incubated for 10 minutes at 20°C.
Blocked using 0.5% milk for 1 minute at 20°C.
Detection method: Western lightning chemiluminescent reagent.
All lanes: Western blot - Anti-ATG8 antibody (AB4753) at 1/1500 dilution
All lanes: Whole cell lysate prepared from Human huh-7 cells expressing the Saccharomyces cerevisiae protein at 15 µg
All lanes: Sheep anti-rabbit IgG conjugated to HRP at 1/7000 dilution
Predicted band size: 14 kDa
Observed band size: 15 kDa
Exposure time: 4min
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