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Rabbit Polyclonal ATG8 antibody. Suitable for ELISA, WB and reacts with Saccharomyces cerevisiae samples. Cited in 10 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C ATG8.


Images

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 6.5
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Tripotassium orthophosphate

Form

Liquid

Clonality

Polyclonal

Immunogen

  • Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C ATG8. Database link P38182
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ELISAWB
Saccharomyces cerevisiae
Expected
Tested

Expected
Expected

Species

Saccharomyces cerevisiae

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Saccharomyces cerevisiae

Dilution info

-

Notes

This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates). Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions.

Target data

Function

Ubiquitin-like modifier involved in cytoplasm to vacuole transport (Cvt) vesicles and autophagosomes formation. With ATG4, mediates the delivery of the vesicles and autophagosomes to the vacuole via the microtubule cytoskeleton. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Participates also in membrane fusion events that take place in the early secretory pathway. Also involved in endoplasmic reticulum-specific autophagic process and is essential for the survival of cells subjected to severe ER stress. The ATG8-PE conjugate mediates tethering between adjacent membranes and stimulates membrane hemifusion, leading to expansion of the autophagosomal membrane during autophagy. Moreover not only conjugation, but also subsequent ATG8-PE deconjugation is an important step required to facilitate multiple events during macroautophagy, and especially for efficient autophagosome biogenesis, the assembly of ATG9-containing tubulovesicular clusters into phagophores/autophagosomes, and for the disassembly of PAS-associated ATG components. Plays also a role in regulation of filamentous growth.

Alternative names

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Rabbit Polyclonal ATG8 antibody. Suitable for ELISA, WB and reacts with Saccharomyces cerevisiae samples. Cited in 10 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C ATG8.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Polyclonal

Immunogen

Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C ATG8. Database link P38182

Purification technique

Ion exchange chromatography

Concentration
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Purification notes

Anti-Apg8 antibody, also known as Atg8, is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.

If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.

Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.

Supplementary info

Biological summary

The ATG8 protein assists in the selective degradation of damaged organelles and proteins through autophagy. It is a member of the ATG ubiquitin-like protein family and works in collaboration with other autophagy proteins such as ATG3 and ATG7. These interactions form complexes necessary for the autophagic process. ATG8 acts as a marker for autophagosomes guiding cargo to be engulfed and recycled effectively.

Mechanical summary

The ATG8 protein also known as autophagy-related protein 8 weighs approximately 14 kDa and plays an important role in autophagy. It is expressed widely in eukaryotic cells including yeast plants and mammals. ATG8 engages in the conjugation with phospholipid phosphatidylethanolamine (PE) on the surface of autophagic vesicles. This conjugation allows the vesicles to elongate and form autophagosomes which are important for the engulfment of cellular components that need to be degraded.

Pathway

ATG8 functions within the autophagy pathway and the lysosome degradation pathway which are essential for cellular maintenance and nutrient recycling. In these pathways ATG8 interacts with proteins like mTOR and ULK1 that regulate autophagy. As a central player it connects upstream signaling events with downstream autophagic membrane dynamics facilitating cellular adaptation in response to nutrient availability and stress conditions.

Disease

Alterations in ATG8 function relate to neurodegenerative diseases such as Parkinson's disease and Huntington's disease. Dysregulation of autophagy where ATG8 plays a critical role impacts protein aggregation and cellular toxicity commonly seen in these conditions. Additionally it connects with proteins like p62 in these disorders as impaired protein clearance pathways influence disease progression. ATG8 involvement highlights its significance in maintaining neuronal health and potentially in therapeutic strategies targeting aberrant autophagic processes.

Product promise

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2 product images

  • Western blot - Anti-ATG8 antibody (ab4753), expandable thumbnail

    Western blot - Anti-ATG8 antibody (ab4753)

    Ab4753, generated by immunization with recombinant yeast ATG8 (or Apg 8), was tested by immunoblot with other anti-UBL (Ubiquitin-like modifier) antibodies against E.coli lysates expressing the ATG8-GFP fusion protein (Apg 8-GFP in both panels)). All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL.

    Panel A shows total protein staining using ponceau.

    Panel B shows specific reaction with ATG8 (Apg 8) using a 1:4,000 and 1:8,000 dilution of ab4753 followed by reaction with a 1:15,000 dilution of HRP Goat-a-Rabbit IgG (ab7090). All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4° C. E.coli lysate proteins were separated by SDS-PAGE using a 15% gel.

    Similar experiments (data not shown), where other UBL fusion proteins were separated and probed with this antibody showed no react

    All lanes: Western blot - Anti-ATG8 antibody (AB4753)

    Predicted band size: 14 kDa

  • Western blot - Anti-ATG8 antibody (ab4753), expandable thumbnail
    This image is courtesy of an anonymous customer review.

    Western blot - Anti-ATG8 antibody (ab4753)

    Gel run under denaturing conditions.
    Primary antibody incubated for 10 minutes at 20°C.
    Blocked using 0.5% milk for 1 minute at 20°C.
    Detection method: Western lightning chemiluminescent reagent.

    All lanes: Western blot - Anti-ATG8 antibody (AB4753) at 1/1500 dilution

    All lanes: Whole cell lysate prepared from Human huh-7 cells expressing the Saccharomyces cerevisiae protein at 15 µg

    Secondary

    All lanes: Sheep anti-rabbit IgG conjugated to HRP at 1/7000 dilution

    Predicted band size: 14 kDa

    Observed band size: 15 kDa

    Exposure time: 4min

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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