Rabbit Polyclonal ATG8 antibody. Suitable for ELISA, WB and reacts with Saccharomyces cerevisiae samples. Cited in 10 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C ATG8.
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Publications
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- Oxidative medicine and cellular longevity 2022:35042792022Astrocytic IGF-1 and IGF-1R Orchestrate Mitophagy in Traumatic Brain Injury via Exosomal miR-let-7e.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRen Dabin,Chen Wei,Shu Liang,Cao Ke,Wang Zhihan,Zheng PingPubMed 36062186
- Autophagy 16:862-8772019Dehydrin MtCAS31 promotes autophagic degradation under drought stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXin Li et. al.PubMed 31362589
- Plant, cell & environment 42:2696-27142019Cadmium induces reactive oxygen species-dependent pexophagy in Arabidopsis leaves.Applications:Unspecified applicationReactive species:Unspecified reactive speciesNieves Calero-Muñoz,Marino Exposito-Rodriguez,Aurelio M Collado-Arenal,María Rodríguez-Serrano,Ana M Laureano-Marín,M Estrella Santamaría,Cecilia Gotor,Isabel Díaz,Philip M Mullineaux,María C Romero-Puertas,Adela Olmedilla,Luisa M SandalioPubMed 31152467
- Plant science : an international journal of experimental plant biology 269:12-192018Autophagy contributes to regulate the ROS levels and PCD progress in TMV-infected tomatoes.Applications:WBReactive species:Unspecified reactive speciesShumin Zhou,Qiang Hong,Yang Li,Qi Li,Mao WangPubMed 29606209
- Genome research 27:1487-15002017Yeast genetic interaction screen of human genes associated with amyotrophic lateral sclerosis: identification of MAP2K5 kinase as a potential drug target.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMyungjin Jo et. al.PubMed 28596290
- The New phytologist 211:886-982016Phosphorus starvation induces membrane remodeling and recycling in Emiliania huxleyi.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAdva Shemi,Daniella Schatz,Helen F Fredricks,Benjamin A S Van Mooy,Ziv Porat,Assaf VardiPubMed 27111716
- Human molecular genetics 25:1100-152016α-Synuclein interferes with the ESCRT-III complex contributing to the pathogenesis of Lewy body disease.Applications:Unspecified applicationReactive species:Unspecified reactive speciesBrian Spencer et. al.PubMed 26740557
- Yeast (Chichester, England) 32:499-5172015Expression of Malus xiaojinensis IRT1 (MxIRT1) protein in transgenic yeast cells leads to degradation through autophagy in the presence of excessive iron.Applications:WBReactive species:Unspecified reactive speciesShuang Li,Xi Zhang,Xiu-Yue Zhang,Wei Xiao,James O Berry,Peng Li,Si Jin,Song Tan,Peng Zhang,Wei-Zhong Zhao,Li-Ping YinPubMed 25871543
- The New phytologist 204:854-632014Hijacking of an autophagy-like process is critical for the life cycle of a DNA virus infecting oceanic algal blooms.Applications:EM, WBReactive species:Unspecified reactive speciesDaniella Schatz et. al.PubMed 25195618
- PloS one 5:e93132010Selective molecular alterations in the autophagy pathway in patients with Lewy body disease and in models of alpha-synucleinopathy.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C ATG8. Database link P38182
Reactivity data
Select an application| ELISA | WB | |
|---|---|---|
| Saccharomyces cerevisiae | Expected | Tested |
ELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Saccharomyces cerevisiae | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Saccharomyces cerevisiae | Dilution info - | Notes This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates). Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions. |
Target data
Function
Ubiquitin-like modifier involved in cytoplasm to vacuole transport (Cvt) vesicles and autophagosome formation (PubMed:10525546, PubMed:10681575, PubMed:10837468, PubMed:11038174, PubMed:11149920, PubMed:16680092, PubMed:18508918, PubMed:19398890, PubMed:21429936, PubMed:7593182, PubMed:8224160). With ATG4, mediates the delivery of the vesicles and autophagosomes to the vacuole via the microtubule cytoskeleton (PubMed:9649430). Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production (PubMed:17404498, PubMed:22768199). Participates also in membrane fusion events that take place in the early secretory pathway (PubMed:10837468). Also involved in endoplasmic reticulum-specific autophagic process and is essential for the survival of cells subjected to severe ER stress (PubMed:17132049). The ATG8-PE conjugate mediates tethering between adjacent membranes and stimulates membrane hemifusion, leading to expansion of the autophagosomal membrane during autophagy (PubMed:11100732, PubMed:17632063, PubMed:20855502). Moreover not only conjugation, but also subsequent ATG8-PE deconjugation is an important step required to facilitate multiple events during macroautophagy, and especially for efficient autophagosome biogenesis, the assembly of ATG9-containing tubulovesicular clusters into phagophores/autophagosomes, and for the disassembly of PAS-associated ATG components (PubMed:17632063, PubMed:20855502, PubMed:22622160). Also plays a role in regulation of filamentous growth (PubMed:17700056). Has a lipidation-independent vacuolar function to facilitate the degradation of vacuolar integral membrane proteins during early-stationary vacuole turnover (EVT) when cells enter stationary phase.
Additional Targets
ATG8
Alternative names
APG8, AUT7, CVT5, YBL078C, YBL0732, ATG8, Autophagy-related protein 8, Autophagy-related ubiquitin-like modifier ATG8, Cytoplasm to vacuole targeting protein 5
Recommended products
Rabbit Polyclonal ATG8 antibody. Suitable for ELISA, WB and reacts with Saccharomyces cerevisiae samples. Cited in 10 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C ATG8.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C ATG8. Database link P38182
- Purification technique
- Ion exchange chromatography
- Concentration
- Purification notes
Anti-Apg8 antibody, also known as Atg8, is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The ATG8 protein also known as autophagy-related protein 8 weighs approximately 14 kDa and plays an important role in autophagy. It is expressed widely in eukaryotic cells including yeast plants and mammals. ATG8 engages in the conjugation with phospholipid phosphatidylethanolamine (PE) on the surface of autophagic vesicles. This conjugation allows the vesicles to elongate and form autophagosomes which are important for the engulfment of cellular components that need to be degraded.
- Biological function summary
The ATG8 protein assists in the selective degradation of damaged organelles and proteins through autophagy. It is a member of the ATG ubiquitin-like protein family and works in collaboration with other autophagy proteins such as ATG3 and ATG7. These interactions form complexes necessary for the autophagic process. ATG8 acts as a marker for autophagosomes guiding cargo to be engulfed and recycled effectively.
- Pathways
ATG8 functions within the autophagy pathway and the lysosome degradation pathway which are essential for cellular maintenance and nutrient recycling. In these pathways ATG8 interacts with proteins like mTOR and ULK1 that regulate autophagy. As a central player it connects upstream signaling events with downstream autophagic membrane dynamics facilitating cellular adaptation in response to nutrient availability and stress conditions.
- Associated diseases and disorders
Alterations in ATG8 function relate to neurodegenerative diseases such as Parkinson's disease and Huntington's disease. Dysregulation of autophagy where ATG8 plays a critical role impacts protein aggregation and cellular toxicity commonly seen in these conditions. Additionally it connects with proteins like p62 in these disorders as impaired protein clearance pathways influence disease progression. ATG8 involvement highlights its significance in maintaining neuronal health and potentially in therapeutic strategies targeting aberrant autophagic processes.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Western blot - Anti-ATG8 antibody (ab4753)
ab4753, generated by immunization with recombinant yeast ATG8 (or Apg 8), was tested by immunoblot with other anti-UBL (Ubiquitin-like modifier) antibodies against E.coli lysates expressing the ATG8-GFP fusion protein (Apg 8-GFP in both panels)). All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL.
Panel A shows total protein staining using ponceau.
Panel B shows specific reaction with ATG8 (Apg 8) using a 1:4,000 and 1:8,000 dilution of ab4753 followed by reaction with a 1:15,000 dilution of HRP Goat-a-Rabbit IgG (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab7090). All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4° C. E.coli lysate proteins were separated by SDS-PAGE using a 15% gel.
Similar experiments (data not shown), where other UBL fusion proteins were separated and probed with this antibody showed no react
All lanes: Western blot - Anti-ATG8 antibody (ab4753)
Predicted band size: 14 kDa
This image is courtesy of an anonymous customer review.Western blot - Anti-ATG8 antibody (ab4753)
Gel run under denaturing conditions.
Primary antibody incubated for 10 minutes at 20°C.
Blocked using 0.5% milk for 1 minute at 20°C.
Detection method: Western lightning chemiluminescent reagent.All lanes: Western blot - Anti-ATG8 antibody (ab4753) at 1/1500 dilution
All lanes: Whole cell lysate prepared from Human huh-7 cells expressing the Saccharomyces cerevisiae protein at 15 µg
SecondaryAll lanes: Sheep anti-rabbit IgG conjugated to HRP at 1/7000 dilution
Predicted band size: 14 kDa
Observed band size: 15 kDa
Exposure time: 4min
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