AB4753

Anti-ATG8 antibody

Name: Anti-ATG8 antibody (ab4753) | Abcam
Brand: Abcam Limited
SKU: AB4753
Rating: 4 (4 reviews)

(4 Reviews)

(12 Publications)

Rabbit Polyclonal ATG8 antibody. Suitable for ELISA, WB and reacts with Saccharomyces cerevisiae samples. Cited in 12 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C ATG8.

View Alternative Names

APG8, AUT7, CVT5, YBL078C, YBL0732, ATG8, Autophagy-related protein 8, Autophagy-related ubiquitin-like modifier ATG8, Cytoplasm to vacuole targeting protein 5

2 Images

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Western blot - Anti-ATG8 antibody (AB4753)

ab4753, generated by immunization with recombinant yeast ATG8 (or Apg 8), was tested by immunoblot with other anti-UBL (Ubiquitin-like modifier) antibodies against E.coli lysates expressing the ATG8-GFP fusion protein (Apg 8-GFP in both panels)). All UBLs possess limited homology to Ubiquitin and to each other, therefore it is important to know the degree of reactivity of each antibody against each UBL.

Panel A shows total protein staining using ponceau.

Panel B shows specific reaction with ATG8 (Apg 8) using a 1 : 4,000 and 1 : 8,000 dilution of ab4753 followed by reaction with a 1 : 15,000 dilution of HRP Goat-a-Rabbit IgG (ab7090). All primary antibodies were diluted in TTBS buffer supplemented with 5% non-fat milk and incubated with the membranes overnight at 4° C. E.coli lysate proteins were separated by SDS-PAGE using a 15% gel.

Similar experiments (data not shown), where other UBL fusion proteins were separated and probed with this antibody showed no react

All lanes:

Western blot - Anti-ATG8 antibody (ab4753)

Predicted band size: 14 kDa

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AbReview16731****

Western blot - Anti-ATG8 antibody (AB4753)

Gel run under denaturing conditions.
Primary antibody incubated for 10 minutes at 20°C.
Blocked using 0.5% milk for 1 minute at 20°C.
Detection method : Western lightning chemiluminescent reagent.

All lanes:

Western blot - Anti-ATG8 antibody (ab4753) at 1/1500 dilution

All lanes:

Whole cell lysate prepared from Human huh-7 cells expressing the Saccharomyces cerevisiae protein at 15 µg

Secondary

All lanes:

Sheep anti-rabbit IgG conjugated to HRP at 1/7000 dilution

Predicted band size: 14 kDa

Observed band size: 15 kDa

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Exposure time: 4min

This image is courtesy of an anonymous abreview.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Saccharomyces cerevisiae

Applications

ELISA, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Saccharomyces cerevisiae S288C ATG8.

P38182

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ELISA" : {"fullname" : "ELISA", "shortname":"ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Saccharomyces cerevisiae": { "ELISA-species-checked": "guaranteed", "ELISA-species-dilution-info": "", "ELISA-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p>This antibody using the specified conditions may recognize other prominent intrinsic bands (UBLs or their conjugates). Other intrinsic bands are readily detectable in yeast lysates at lower antibody dilutions.</p>" } } }

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Properties and storage information

Form

Liquid

Purity

IgG fraction

Purification technique

Ion exchange chromatography

Purification notes

Anti-Apg8 antibody, also known as Atg8, is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer.

Storage buffer

Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ATG8 protein also known as autophagy-related protein 8 weighs approximately 14 kDa and plays an important role in autophagy. It is expressed widely in eukaryotic cells including yeast plants and mammals. ATG8 engages in the conjugation with phospholipid phosphatidylethanolamine (PE) on the surface of autophagic vesicles. This conjugation allows the vesicles to elongate and form autophagosomes which are important for the engulfment of cellular components that need to be degraded.

Biological function summary

The ATG8 protein assists in the selective degradation of damaged organelles and proteins through autophagy. It is a member of the ATG ubiquitin-like protein family and works in collaboration with other autophagy proteins such as ATG3 and ATG7. These interactions form complexes necessary for the autophagic process. ATG8 acts as a marker for autophagosomes guiding cargo to be engulfed and recycled effectively.

Pathways

ATG8 functions within the autophagy pathway and the lysosome degradation pathway which are essential for cellular maintenance and nutrient recycling. In these pathways ATG8 interacts with proteins like mTOR and ULK1 that regulate autophagy. As a central player it connects upstream signaling events with downstream autophagic membrane dynamics facilitating cellular adaptation in response to nutrient availability and stress conditions.

Alterations in ATG8 function relate to neurodegenerative diseases such as Parkinson's disease and Huntington's disease. Dysregulation of autophagy where ATG8 plays a critical role impacts protein aggregation and cellular toxicity commonly seen in these conditions. Additionally it connects with proteins like p62 in these disorders as impaired protein clearance pathways influence disease progression. ATG8 involvement highlights its significance in maintaining neuronal health and potentially in therapeutic strategies targeting aberrant autophagic processes.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Ubiquitin-like modifier involved in cytoplasm to vacuole transport (Cvt) vesicles and autophagosome formation (PubMed : 10525546, PubMed : 10681575, PubMed : 10837468, PubMed : 11038174, PubMed : 11149920, PubMed : 16680092, PubMed : 18508918, PubMed : 19398890, PubMed : 21429936, PubMed : 7593182, PubMed : 8224160). With ATG4, mediates the delivery of the vesicles and autophagosomes to the vacuole via the microtubule cytoskeleton (PubMed : 9649430). Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production (PubMed : 17404498, PubMed : 22768199). Participates also in membrane fusion events that take place in the early secretory pathway (PubMed : 10837468). Also involved in endoplasmic reticulum-specific autophagic process and is essential for the survival of cells subjected to severe ER stress (PubMed : 17132049). The ATG8-PE conjugate mediates tethering between adjacent membranes and stimulates membrane hemifusion, leading to expansion of the autophagosomal membrane during autophagy (PubMed : 11100732, PubMed : 17632063, PubMed : 20855502). Moreover not only conjugation, but also subsequent ATG8-PE deconjugation is an important step required to facilitate multiple events during macroautophagy, and especially for efficient autophagosome biogenesis, the assembly of ATG9-containing tubulovesicular clusters into phagophores/autophagosomes, and for the disassembly of PAS-associated ATG components (PubMed : 17632063, PubMed : 20855502, PubMed : 22622160). Also plays a role in regulation of filamentous growth (PubMed : 17700056).. Has a lipidation-independent vacuolar function to facilitate the degradation of vacuolar integral membrane proteins during early-stationary vacuole turnover (EVT) when cells enter stationary phase.

See full target information ATG8

Additional targets

ATG8

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Publications (12)

Recent publications for all applications. Explore the full list and refine your search

Biomaterials research 29:0201 PubMed40330275

2025

Impact of Culture Duration on the Properties and Functionality of Yeast-Derived Extracellular Vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Gyeongchan Jeon,Yang-Hoon Kim,Jiho Min

Heliyon 10:e35131 PubMed39157375

2024

IL-37 attenuated HPV induced inflammation and growth of oral epithelial cells via regulating autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Yahong Shi,Wenjing Wang,Yunfang Bai,Xiaoying Liu,Liwei Wu,Ning Liu

Oxidative medicine and cellular longevity 2022:3504279 PubMed36062186

2022

Astrocytic IGF-1 and IGF-1R Orchestrate Mitophagy in Traumatic Brain Injury via Exosomal miR-let-7e.

Applications

Unspecified application

Species

Unspecified reactive species

Ren Dabin,Chen Wei,Shu Liang,Cao Ke,Wang Zhihan,Zheng Ping

Autophagy 16:862-877 PubMed31362589

2019

Dehydrin MtCAS31 promotes autophagic degradation under drought stress.

Applications

Unspecified application

Species

Unspecified reactive species

Xin Li,Qianwen Liu,Hao Feng,Jie Deng,Rongxue Zhang,Jiangqi Wen,Jiangli Dong,Tao Wang

Plant, cell & environment 42:2696-2714 PubMed31152467

2019

Cadmium induces reactive oxygen species-dependent pexophagy in Arabidopsis leaves.

Applications

Unspecified application

Species

Unspecified reactive species

Nieves Calero-Muñoz,Marino Exposito-Rodriguez,Aurelio M Collado-Arenal,María Rodríguez-Serrano,Ana M Laureano-Marín,M Estrella Santamaría,Cecilia Gotor,Isabel Díaz,Philip M Mullineaux,María C Romero-Puertas,Adela Olmedilla,Luisa M Sandalio

Plant science : an international journal of experimental plant biology 269:12-19 PubMed29606209

2018

Autophagy contributes to regulate the ROS levels and PCD progress in TMV-infected tomatoes.

Applications

Species

Unspecified reactive species

Shumin Zhou,Qiang Hong,Yang Li,Qi Li,Mao Wang

Genome research 27:1487-1500 PubMed28596290

2017

Yeast genetic interaction screen of human genes associated with amyotrophic lateral sclerosis: identification of MAP2K5 kinase as a potential drug target.

Applications

Unspecified application

Species

Unspecified reactive species

Myungjin Jo,Ah Young Chung,Nozomu Yachie,Minchul Seo,Hyejin Jeon,Youngpyo Nam,Yeojin Seo,Eunmi Kim,Quan Zhong,Marc Vidal,Hae Chul Park,Frederick P Roth,Kyoungho Suk

The New phytologist 211:886-98 PubMed27111716

2016

Phosphorus starvation induces membrane remodeling and recycling in Emiliania huxleyi.

Applications

Unspecified application

Species

Unspecified reactive species

Adva Shemi,Daniella Schatz,Helen F Fredricks,Benjamin A S Van Mooy,Ziv Porat,Assaf Vardi

Human molecular genetics 25:1100-15 PubMed26740557

2016

α-Synuclein interferes with the ESCRT-III complex contributing to the pathogenesis of Lewy body disease.

Applications

Unspecified application

Species

Unspecified reactive species

Brian Spencer,Changyoun Kim,Tania Gonzalez,Alejandro Bisquertt,Christina Patrick,Edward Rockenstein,Anthony Adame,Seung-Jae Lee,Paula Desplats,Eliezer Masliah

Yeast (Chichester, England) 32:499-517 PubMed25871543

2015

Expression of Malus xiaojinensis IRT1 (MxIRT1) protein in transgenic yeast cells leads to degradation through autophagy in the presence of excessive iron.

Applications

Species

Unspecified reactive species

Shuang Li,Xi Zhang,Xiu-Yue Zhang,Wei Xiao,James O Berry,Peng Li,Si Jin,Song Tan,Peng Zhang,Wei-Zhong Zhao,Li-Ping Yin

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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Anti-ATG8 antibody

Western blot - Anti-ATG8 antibody (AB4753)

Western blot - Anti-ATG8 antibody (AB4753)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purity

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (12)

Complementary Products

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Primary Antibodies

Primary Antibodies

Primary Antibodies

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