JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB229334

Anti-ATG9A antibody - C-terminal

Be the first to review this product! Submit a review

|

(2 Publications)

Rabbit Polyclonal ATG9A antibody. C-terminal. Suitable for IP, WB, IHC-P, ICC/IF and reacts with Human, Mouse, Rat samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ATG9A.

View Alternative Names

APG9L1, ATG9A, Autophagy-related protein 9A, APG9-like 1, mATG9

7 Images
Immunocytochemistry/ Immunofluorescence - Anti-ATG9A antibody - C-terminal (AB229334)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ATG9A antibody - C-terminal (AB229334)

A431 (human epidermoid carcinoma cell line) cells were stained for ATG9A (green) using ab229334 at 1/500 dilution in ICC/IF. Cells were fixed in 100% methanol for 5 minutes.

Nuclear counterstain : Hoechst 33342 (blue).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG9A antibody - C-terminal (AB229334)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG9A antibody - C-terminal (AB229334)

Paraffin-embedded human colon carcinoma tissue stained for ATG9A using ab229334 at 1/500 dilution in immunohistochemical analysis.

Immunoprecipitation - Anti-ATG9A antibody - C-terminal (AB229334)
  • IP

Supplier Data

Immunoprecipitation - Anti-ATG9A antibody - C-terminal (AB229334)

ATG9A was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract with 4 μg of ab229334. Western blot was performed from the immunoprecipitate using ab229334 at 1/500 dilution.

Lane 1 : 4 μg preimmune rabbit igG instead of ab229334 in HeLa whole cell extract.
Lane 2 : ab229334 IP in HeLa whole cell extract.

7.5% SDS-PAGE gel.

All lanes:

Immunoprecipitation - Anti-ATG9A antibody - C-terminal (ab229334)

Predicted band size: 94 kDa

false

Western blot - Anti-ATG9A antibody - C-terminal (AB229334)
  • WB

Supplier Data

Western blot - Anti-ATG9A antibody - C-terminal (AB229334)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-ATG9A antibody - C-terminal (ab229334) at 1/1000 dilution

All lanes:

RD (human rhabdomyosarcoma cell line) whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 94 kDa

false

Western blot - Anti-ATG9A antibody - C-terminal (AB229334)
  • WB

Supplier Data

Western blot - Anti-ATG9A antibody - C-terminal (AB229334)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-ATG9A antibody - C-terminal (ab229334) at 1/1000 dilution

Lane 1:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Lane 2:

A431 (human epidermoid carcinoma cell line) whole cell lysate at 30 µg

Lane 3:

HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 94 kDa

false

Western blot - Anti-ATG9A antibody - C-terminal (AB229334)
  • WB

Supplier Data

Western blot - Anti-ATG9A antibody - C-terminal (AB229334)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-ATG9A antibody - C-terminal (ab229334) at 1/1000 dilution

All lanes:

BCL-1 (mouse B lymphocyte lymphoma cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 94 kDa

false

Western blot - Anti-ATG9A antibody - C-terminal (AB229334)
  • WB

Supplier Data

Western blot - Anti-ATG9A antibody - C-terminal (AB229334)

7.5% SDS-PAGE gel.

All lanes:

Western blot - Anti-ATG9A antibody - C-terminal (ab229334) at 1/1000 dilution

Lane 1:

PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate at 30 µg

Lane 2:

Rat2 (rat fibroblast cell line) whole cell lysate at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

Predicted band size: 94 kDa

false

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

WB, IP, IHC-P, ICC/IF

applications

Immunogen

Synthetic Peptide within Human ATG9A. The exact immunogen used to generate this antibody is proprietary information.

Q7Z3C6

style
left-column

Alternative Products

Primary Antibodies

AB108338

Anti-ATG9A antibody [EPR2450(2)]

RabMAb|Recombinant|KO Validated|20ul selling size|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-ATG9A antibody [EPR2450(2)] (AB108338)

  • 5
  • 15
Primary Antibodies

AB206253

Alexa Fluor® 647 Anti-ATG9A antibody [EPR2450(2)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-ATG9A antibody [EPR2450(2)] (AB206253)

  • 0
  • 0
Primary Antibodies

AB206252

Alexa Fluor® 488 Anti-ATG9A antibody [EPR2450(2)]

RabMAb|Recombinant

Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 488 Anti-ATG9A antibody [EPR2450(2)] (AB206252)

  • 0
  • 0
Primary Antibodies

AB223528

Anti-ATG9A antibody [EPR2450(2)] - BSA and Azide free

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-ATG9A antibody [EPR2450(2)] - BSA and Azide free (AB223528)

  • 0
  • 0

Complementary Products

Primary Antibodies

AB192890

Anti-LC3B antibody [EPR18709] - Autophagosome Marker

BOND RX™ Validated|RabMAb|Recombinant|KO Validated|Lab Essentials|20ul selling size

Western blot - Anti-LC3B antibody [EPR18709] - Autophagosome Marker (AB192890)

  • 5
  • 19
Primary Antibodies

AB108327

Anti-APG5L/ATG5 antibody [EPR1755(2)]

20ul selling size|RabMAb|Recombinant|KO Validated|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-APG5L/ATG5 antibody [EPR1755(2)] (AB108327)

  • 4
  • 7
Multiplex Kits

AB228525

Autophagy Marker (APG5L/ATG5, ATG16L1, ATG4B, ATG9A, Beclin 1, LC3B) Antibody Sampler Panel

Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Autophagy Marker (APG5L/ATG5, ATG16L1, ATG4B, ATG9A, Beclin 1, LC3B) Antibody Sampler Panel (AB228525)

  • 5
  • 1
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/100 - 1/500", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100 - 1/1000", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Cow": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATG9A also known as APG9-like 1 or ATG9 autophagy related 9A is an important component in the autophagy machinery. This protein has a molecular weight of approximately 93 kDa. It is ubiquitously expressed in various tissues with higher expression seen in the heart and skeletal muscle. Mechanically ATG9A is essential for the trafficking of membranes necessary for autophagosome formation. It actively participates in membrane lipids' delivery from donor organelles which are critical for constructing autophagosomes.
Biological function summary

ATG9A facilitates the recycling of cellular materials through a process called autophagy. This protein acts with other autophagy-related proteins to form a complex essential for autophagosome elongation and closure. The ATG9A protein's dynamic movements between the Golgi apparatus and endosomes ensure proper membrane supply for autophagy. It coordinates with the ULK1 complex and various phospholipid-modifying enzymes which are necessary to regulate autophagic flux.

Pathways

ATG9A plays an integral role in the mTOR signaling and MAPK pathways. Both pathways are essential for cellular responses to stress and nutrient availability. In these pathways ATG9A works closely with proteins like beclin 1 and ATG5. Inhibiting mTOR actively induces autophagy where ATG9A contributes to membrane recruitment and elongation of the autophagosomes facilitating the clearance of damaged organelles and proteins.

ATG9A shows a significant link to neurodegenerative diseases such as Parkinson's and Alzheimer's disease. The dysfunction of autophagic pathways due to altered ATG9A function leads to the accumulation of damaged proteins and organelles. This event contributes to the pathogenesis of such neurodegenerative diseases. Moreover ATG9A closely associates with proteins like LAMP2 whose mutations similarly lead to defective autophagic processes resulting in the accumulation of unprocessed cellular debris in these disorders.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Phospholipid scramblase involved in autophagy by mediating autophagosomal membrane expansion (PubMed : 22456507, PubMed : 27510922, PubMed : 29437695, PubMed : 32513819, PubMed : 32610138, PubMed : 33106659, PubMed : 33468622, PubMed : 33850023). Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome (PubMed : 16940348, PubMed : 22456507, PubMed : 33106659). Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 (ATG2A or ATG2B) from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion (PubMed : 33106659). Also required to supply phosphatidylinositol 4-phosphate to the autophagosome initiation site by recruiting the phosphatidylinositol 4-kinase beta (PI4KB) in a process dependent on ARFIP2, but not ARFIP1 (PubMed : 30917996). In addition to autophagy, also plays a role in necrotic cell death (By similarity).
See full target information ATG9A
style
left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in pharmacology 14:1307447 PubMed38089055

2023

Neuroprotective effects of Neurotrophin-3 in MPTP-induced zebrafish Parkinson's disease model.

Applications

Unspecified application

Species

Unspecified reactive species

Noor Azzizah Omar,Jaya Kumar,Seong Lin Teoh

iScience 26:106995 PubMed37534135

2023

Robust induction of functional astrocytes using NGN2 expression in human pluripotent stem cells.

Applications

Unspecified application

Species

Unspecified reactive species

Martin H Berryer,Matthew Tegtmeyer,Loïc Binan,Vera Valakh,Anna Nathanson,Darina Trendafilova,Ethan Crouse,Jenny A Klein,Daniel Meyer,Olli Pietiläinen,Francesca Rapino,Samouil L Farhi,Lee L Rubin,Steven A McCarroll,Ralda Nehme,Lindy E Barrett
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com