JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB117591

Anti-ATG9B antibody

5

(4 Reviews)

|

(6 Publications)

Rabbit Polyclonal ATG9B antibody. Suitable for IHC-P and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Synthetic Peptide within Human ATG9B conjugated to Keyhole Limpet Haemocyanin.

View Alternative Names

APG9L2, NOS3AS, ATG9B, Autophagy-related protein 9B, APG9-like 2, Nitric oxide synthase 3-overlapping antisense gene protein, Protein sONE

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG9B antibody (AB117591)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG9B antibody (AB117591)

Formalin fixed, paraffin embedded Human spleen tissue labelled with ab117591 at 5 µg/ml

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG9B antibody (AB117591)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATG9B antibody (AB117591)

Formalin fixed, paraffin embedded Human placenta tissue labelled with ab117591 at 5 μg/ml

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P

applications

Immunogen

Synthetic Peptide within Human ATG9B conjugated to Keyhole Limpet Haemocyanin. The exact immunogen used to generate this antibody is proprietary information.

Q674R7

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "5 µg/mL", "IHCP-species-notes": "<p></p>" }, "Mouse": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
style
left-column
style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.4 Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATG9B also known as APG9L2 plays a central role in autophagy. This protein has an approximate mass of 106 kDa and is primarily expressed in the human brain and heart with lower levels found in other tissues. Functionally ATG9B interacts with cellular membranes and is involved in trafficking between organelles helping to form autophagosomes. While studying its mechanisms researchers have found its expression patterns closely link to the areas where metabolic regulation is critical.
Biological function summary

Proteins like ATG9B facilitate cellular homeostasis by modulating autophagic response activities. ATG9B is a core component of the autophagy machinery; it localizes to the trans-Golgi network and endosomes playing a regulatory function in the early stages of autophagosome formation. As part of the larger ATG protein complex it ensures proper vesicle nucleation and elongation required for autophagosome maturation. This involvement highlights its pivotal role in maintaining cellular health especially under stressful conditions.

Pathways

ATG9B is significant in the macroautophagy and mTOR signaling pathways. ATG9B functions closely with ATG7 and ATG12 which are essential for autophagosome efficiency in the macroautophagy pathway. The mTOR pathway which regulates cell growth and metabolism responding to nutrients energy and stress can influence ATG9B actions. This interconnectedness explains how cells adjust their metabolic throughput under conditions like nutrient scarcity or hypoxic stress highlighting the complexity of these cellular survival mechanisms.

Scientists have linked ATG9B to neurodegenerative diseases and cancer. Impaired autophagic roles or dysfunctional regulation associated with ATG9B can lead to conditions like Huntington's disease where protein aggregates accumulate due to insufficient degradation. In cancer ATG9B's role diverges where it may either suppress or support tumorigenesis depending on the cellular context. The relationship with proteins such as p53 in cancer emphasizes how ATG9B potentially influences cell death and survival pathways making it of interest for therapeutic studies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Phospholipid scramblase involved in autophagy by mediating autophagosomal membrane expansion. Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome. Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 (ATG2A or ATG2B) from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion (By similarity). In addition to autophagy, also plays a role in necrotic cell death (By similarity).
See full target information ATG9B
style
left-column

Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Clinical and experimental reproductive medicine 51:301-308 PubMed38757275

2024

The differential expression patterns of Atg9a and Atg9b in cells of the reproductive organs.

Applications

Unspecified application

Species

Unspecified reactive species

Minseo Lee,Sujin Son,Hyunjung J Lim,Haengseok Song

Autophagy 20:557-576 PubMed37938170

2023

ATG9B is a tissue-specific homotrimeric lipid scramblase that can compensate for ATG9A.

Applications

Unspecified application

Species

Unspecified reactive species

George N Chiduza,Acely Garza-Garcia,Eugenia Almacellas,Stefano De Tito,Valerie E Pye,Alexander R van Vliet,Peter Cherepanov,Sharon A Tooze

Aging cell 19:e13187 PubMed32627317

2020

Aging is associated with a decline in Atg9b-mediated autophagosome formation and appearance of enlarged mitochondria in the heart.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Liang,Alexandra G Moyzis,Mark A Lampert,Rachel Y Diao,Rita H Najor,Åsa B Gustafsson

The Journal of biological chemistry 294:20009-20023 PubMed31748416

2019

Excessive ER-phagy mediated by the autophagy receptor FAM134B results in ER stress, the unfolded protein response, and cell death in HeLa cells.

Applications

Unspecified application

Species

Unspecified reactive species

Yangjie Liao,Bo Duan,Yufei Zhang,Xinmin Zhang,Bin Xia

Biochemical and biophysical research communication 512:236-243 PubMed30885435

2019

Alliin alleviates myocardial ischemia-reperfusion injury by promoting autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Zhao,Enzehua Xie,Xiubin Yang,Bing Gong

Bioscience reports 37: PubMed28923830

2017

The role of and expression in clear cell renal cell carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Zheng Ma,Zengguang Qi,Zhengfei Shan,Jiangsong Li,Jing Yang,Zhonghua Xu
View all publications
style
left-column
style
left-column
style
right-column

Alternative Products

Primary Antibodies

AB240897

Anti-ATG9B antibody

Immunocytochemistry/ Immunofluorescence - Anti-ATG9B antibody (AB240897)

  • 1
  • 1

Complementary Products

Primary Antibodies

AB108338

Anti-ATG9A antibody [EPR2450(2)]

RabMAb|Recombinant|KO Validated|20ul selling size|Trial Size

Immunocytochemistry/ Immunofluorescence - Anti-ATG9A antibody [EPR2450(2)] (AB108338)

  • 5
  • 14
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB6046

Anti-beta Tubulin antibody - Loading Control

BOND RX™ Validated

Immunoprecipitation - Anti-beta Tubulin antibody - Loading Control (AB6046)

  • 5
  • 61
Primary Antibodies

AB109012

Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker

KO Validated|RabMAb|Recombinant|Lab Essentials

Western blot - Anti-SQSTM1 / p62 antibody [EPR4844] - Autophagosome Marker (AB109012)

  • 5
  • 15

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com