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AB240897

Anti-ATG9B antibody

1

(1 Review)

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(2 Publications)

Rabbit Polyclonal ATG9B antibody. Suitable for WB, ICC/IF and reacts with Rat, Human samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human ATG9B aa 100-150.

View Alternative Names

APG9L2, NOS3AS, ATG9B, Autophagy-related protein 9B, APG9-like 2, Nitric oxide synthase 3-overlapping antisense gene protein, Protein sONE

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-ATG9B antibody (AB240897)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ATG9B antibody (AB240897)

Immunofluorescent analysis of 4% paraformaldehyde-fixed SK-N-BE (human neuroblastoma cell line) cells labeling ATG9B with ab240897 at 1/100 dilution for 60 minutes at room temperature, followed by Goat Anti-Rabbit ATTO 488 at 1/200 dilution for 60 minutes (green). The nuclear counter stain is DAPI (blue). Actin is detected with Phalloidin Texas Red F-Actin stain (red).

A : DAPI, B : Actin, C : ATG9B, D : Composite.

Western blot - Anti-ATG9B antibody (AB240897)
  • WB

Supplier Data

Western blot - Anti-ATG9B antibody (AB240897)

Primary incubation for 16 hours at 4°C.

All lanes:

Western blot - Anti-ATG9B antibody (ab240897) at 1/1000 dilution

All lanes:

Rat brain lysate at 15 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG: HRP at 1/2000 dilution

Predicted band size: 101 kDa

true

Exposure time: 6min

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

WB, ICC/IF

applications

Immunogen

Synthetic Peptide within Human ATG9B aa 100-150. The exact immunogen used to generate this antibody is proprietary information.

Q674R7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
Preservative: 0.09% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATG9B also known as APG9L2 plays a central role in autophagy. This protein has an approximate mass of 106 kDa and is primarily expressed in the human brain and heart with lower levels found in other tissues. Functionally ATG9B interacts with cellular membranes and is involved in trafficking between organelles helping to form autophagosomes. While studying its mechanisms researchers have found its expression patterns closely link to the areas where metabolic regulation is critical.
Biological function summary

Proteins like ATG9B facilitate cellular homeostasis by modulating autophagic response activities. ATG9B is a core component of the autophagy machinery; it localizes to the trans-Golgi network and endosomes playing a regulatory function in the early stages of autophagosome formation. As part of the larger ATG protein complex it ensures proper vesicle nucleation and elongation required for autophagosome maturation. This involvement highlights its pivotal role in maintaining cellular health especially under stressful conditions.

Pathways

ATG9B is significant in the macroautophagy and mTOR signaling pathways. ATG9B functions closely with ATG7 and ATG12 which are essential for autophagosome efficiency in the macroautophagy pathway. The mTOR pathway which regulates cell growth and metabolism responding to nutrients energy and stress can influence ATG9B actions. This interconnectedness explains how cells adjust their metabolic throughput under conditions like nutrient scarcity or hypoxic stress highlighting the complexity of these cellular survival mechanisms.

Scientists have linked ATG9B to neurodegenerative diseases and cancer. Impaired autophagic roles or dysfunctional regulation associated with ATG9B can lead to conditions like Huntington's disease where protein aggregates accumulate due to insufficient degradation. In cancer ATG9B's role diverges where it may either suppress or support tumorigenesis depending on the cellular context. The relationship with proteins such as p53 in cancer emphasizes how ATG9B potentially influences cell death and survival pathways making it of interest for therapeutic studies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Phospholipid scramblase involved in autophagy by mediating autophagosomal membrane expansion. Cycles between the preautophagosomal structure/phagophore assembly site (PAS) and the cytoplasmic vesicle pool and supplies membrane for the growing autophagosome. Lipid scramblase activity plays a key role in preautophagosomal structure/phagophore assembly by distributing the phospholipids that arrive through ATG2 (ATG2A or ATG2B) from the cytoplasmic to the luminal leaflet of the bilayer, thereby driving autophagosomal membrane expansion (By similarity). In addition to autophagy, also plays a role in necrotic cell death (By similarity).
See full target information ATG9B
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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in cell and developmental biology 13:1582970 PubMed40677921

2025

Selective blockade of acid-sensing ion channel 1a can provide substantial hippocampal neuroprotection.

Applications

Unspecified application

Species

Unspecified reactive species

Jiaai Li,Yu Cheng,Di Ma,Guangjian Li,Weixuan Zhao,Ting Jiang,Hongmei Meng

Scientific reports 15:10240 PubMed40133377

2025

Has-miR-30c-1-3p inhibits macrophage autophagy and promotes Mycobacterium tuberculosis survival by targeting ATG4B and ATG9B.

Applications

Unspecified application

Species

Unspecified reactive species

Xianglin Peng,Feifei Pu,Fangzheng Zhou,Xiyong Dai,Feng Xu,Junwen Wang,Jing Feng,Ping Xia
View all publications
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Product promise

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