AB205302

Anti-ATHL1 antibody [EPR13421]

RabMAb
Recombinant
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(1 Publication)

Rabbit Recombinant Monoclonal ATHL1 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ATHL1, PGGHG, Protein-glucosylgalactosylhydroxylysine glucosidase, Acid trehalase-like protein 1

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-ATHL1 antibody [EPR13421] (AB205302)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ATHL1 antibody [EPR13421] (AB205302)

Immunofluorescent analysis of 100% Methanol-fixed 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling ATHL1 with ab205302 at 1/250 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic staining on MCF-7 cell line.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows : -

-ve control 1 : ab205302 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ATHL1 antibody [EPR13421] (AB205302)

Immunofluorescent analysis of 100% Methanol-fixed 0.1% Triton X-100 permeabilized BxPC-3 (Human pancreas adenocarcinoma cells) cells labeling ATHL1 with ab205302 at 1/250 dilution followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic staining on BxPC-3 cell line.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows : -

Supplier Data

Western blot - Anti-ATHL1 antibody [EPR13421] (AB205302)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ATHL1 antibody [EPR13421] (ab205302) at 1/1000 dilution

All lanes:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 81 kDa

Observed band size: 80 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-ATHL1 antibody [EPR13421] (AB205302)

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ATHL1 antibody [EPR13421] (ab205302) at 1/1000 dilution

Lane 1:

Human fetal liver tissue lysate at 10 µg

Lane 2:

BxPC-3 (Human pancreas adenocarcinoma cells) whole cell lysate at 10 µg

Lane 3:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 81 kDa

Observed band size: 80 kDa

false

Exposure time: 1min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13421

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "<p></p>" } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATHL1 also known as ATHL1 protein is a protein with a molecular weight of approximately 70 kDa. Known to be expressed largely in the human brain ATHL1 plays a role in various cellular functions. It is found distributed in other tissues as well but with lower levels noted. This protein interacts with cellular pathways and processes helping in the regulation of key molecular functions.

Biological function summary

ATHL1 engages with cytoskeletal elements and impact cellular structure and stability. It does not form part of a larger protein complex but acts through a collaborative network with other proteins. Its interactions support various cellular processes by influencing the components involved in maintaining cellular shape and integrity. ATHL1's role in these processes makes it an important element in the understanding of cellular dynamics.

Pathways

ATHL1 gets involved in the regulation of the MAPK/ERK pathway. It links with critical components like ERK1/2 affecting cell growth and survival. Another significant pathway involving ATHL1 is the Wnt signaling pathway. This pathway important for numerous developmental processes sees ATHL1 contributing to cellular signaling cascades that determine cell fate and proliferation.

ATHL1 has links to neurological conditions notably Alzheimer's disease. It potentially interacts with tau protein which is known for its role in neurodegenerative processes. Additionally ATHL1's involvement in cellular structural integrity suggests potential ties to cancer where disruptions in cell framework can lead to malignant transformations. Its connections with cytoskeletal proteins highlight ATHL1's role in disease mechanisms and offer avenues for therapeutic exploration.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the hydrolysis of glucose from the disaccharide unit linked to hydroxylysine residues of collagen and collagen-like proteins.

See full target information PGGHG

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Cell communication and signaling : CCS 23:412 PubMed41039499

2025

A comparative proteomic, transcriptomic and glycomic analysis of extracellular vesicle isolation techniques highlights ExoGAG efficiency for a more complete identification of breast milk molecular signaling pathways.

Applications

Unspecified application

Species

Unspecified reactive species

María Pereira-Hernández,Rosaura Picáns-Leis,María E Vázquez-Mosquera,Nerea Lago-Baameiro,Pedro Fortes-González,Laura López-Valverde,Ana Barcia de la Iglesia,Laura Núñez-González,Susana B Bravo,María Pardo,María L Couce,Miguel A Garcia-Gonzalez

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATHL1 antibody [EPR13421]

Immunocytochemistry/ Immunofluorescence - Anti-ATHL1 antibody [EPR13421] (AB205302)

Immunocytochemistry/ Immunofluorescence - Anti-ATHL1 antibody [EPR13421] (AB205302)

Western blot - Anti-ATHL1 antibody [EPR13421] (AB205302)

Western blot - Anti-ATHL1 antibody [EPR13421] (AB205302)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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Secondary Antibodies

Primary Antibodies

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Product promise