AB251424

Anti-ATHL1 antibody [EPR13421] - BSA and Azide free

RabMAb
Recombinant
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Rabbit Recombinant Monoclonal ATHL1 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples.

View Alternative Names

ATHL1, PGGHG, Protein-glucosylgalactosylhydroxylysine glucosidase, Acid trehalase-like protein 1

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-ATHL1 antibody [EPR13421] - BSA and Azide free (AB251424)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ATHL1 antibody [EPR13421] - BSA and Azide free (AB251424)

This data was developed using ab205302, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 100% Methanol-fixed, 0.1% Triton X-100 permeabilized MCF7 (Human breast adenocarcinoma cell line) cells labeling ATHL1 with ab205302 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic staining on MCF-7 cell line.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows : -

-ve control 1 : ab205302 at 1/250 dilution followed by ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution.
-ve control 2 : ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution followed by ab150077 (Alexa Fluor®488 Goat Anti-Rabbit IgG H&L) at 1/1000 dilution.

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-ATHL1 antibody [EPR13421] - BSA and Azide free (AB251424)

This data was developed using ab205302, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 100% Methanol-fixed, 0.1% Triton X-100 permeabilized BxPC-3 (Human pancreas adenocarcinoma cells) cells labeling ATHL1 with ab205302 at 1/250 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).

Confocal image showing cytoplasmic staining on BxPC-3 cell line.

The nuclear counterstain is DAPI (blue).

Tubulin is detected with ab7291 (anti-Tubulin mouse mAb) at 1/1000 dilution and ab150120 (AlexaFluor®594 Goat anti-Mouse secondary) at 1/1000 dilution (red).

The negative controls are as follows : -

Supplier Data

Western blot - Anti-ATHL1 antibody [EPR13421] - BSA and Azide free (AB251424)

This data was developed using ab205302, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ATHL1 antibody [EPR13421] (<a href='/en-us/products/primary-antibodies/athl1-antibody-epr13421-ab205302'>ab205302</a>) at 1/1000 dilution

All lanes:

Human fetal kidney lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 81 kDa

Observed band size: 80 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-ATHL1 antibody [EPR13421] - BSA and Azide free (AB251424)

This data was developed using ab205302, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ATHL1 antibody [EPR13421] (<a href='/en-us/products/primary-antibodies/athl1-antibody-epr13421-ab205302'>ab205302</a>) at 1/1000 dilution

Lane 1:

Human fetal liver tissue lysate at 10 µg

Lane 2:

BxPC-3 (Human pancreas adenocarcinoma cells) whole cell lysate at 10 µg

Lane 3:

MCF7 (Human breast adenocarcinoma cell line) whole cell lysate at 10 µg

Secondary

All lanes:

Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/10000 dilution

Predicted band size: 81 kDa

Observed band size: 80 kDa

false

Exposure time: 1min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13421

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Product details

ab251424 is the carrier-free version of ab205302.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATHL1 also known as ATHL1 protein is a protein with a molecular weight of approximately 70 kDa. Known to be expressed largely in the human brain ATHL1 plays a role in various cellular functions. It is found distributed in other tissues as well but with lower levels noted. This protein interacts with cellular pathways and processes helping in the regulation of key molecular functions.

Biological function summary

ATHL1 engages with cytoskeletal elements and impact cellular structure and stability. It does not form part of a larger protein complex but acts through a collaborative network with other proteins. Its interactions support various cellular processes by influencing the components involved in maintaining cellular shape and integrity. ATHL1's role in these processes makes it an important element in the understanding of cellular dynamics.

Pathways

ATHL1 gets involved in the regulation of the MAPK/ERK pathway. It links with critical components like ERK1/2 affecting cell growth and survival. Another significant pathway involving ATHL1 is the Wnt signaling pathway. This pathway important for numerous developmental processes sees ATHL1 contributing to cellular signaling cascades that determine cell fate and proliferation.

ATHL1 has links to neurological conditions notably Alzheimer's disease. It potentially interacts with tau protein which is known for its role in neurodegenerative processes. Additionally ATHL1's involvement in cellular structural integrity suggests potential ties to cancer where disruptions in cell framework can lead to malignant transformations. Its connections with cytoskeletal proteins highlight ATHL1's role in disease mechanisms and offer avenues for therapeutic exploration.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the hydrolysis of glucose from the disaccharide unit linked to hydroxylysine residues of collagen and collagen-like proteins.

See full target information PGGHG

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATHL1 antibody [EPR13421] - BSA and Azide free

Immunocytochemistry/ Immunofluorescence - Anti-ATHL1 antibody [EPR13421] - BSA and Azide free (AB251424)

Immunocytochemistry/ Immunofluorescence - Anti-ATHL1 antibody [EPR13421] - BSA and Azide free (AB251424)

Western blot - Anti-ATHL1 antibody [EPR13421] - BSA and Azide free (AB251424)

Western blot - Anti-ATHL1 antibody [EPR13421] - BSA and Azide free (AB251424)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Complementary Products

Primary Antibodies

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Product promise