AB188321

Anti-ATIC antibody [EPR13243-53]

RabMAb
Recombinant
What is this?

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(2 Publications)

Rabbit Recombinant Monoclonal ATIC antibody. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 2 publications.

View Alternative Names

PURH, OK/SW-cl.86, ATIC, Bifunctional purine biosynthesis protein ATIC, AICAR transformylase/inosine monophosphate cyclohydrolase

3 Images

Flow Cytometry (Intracellular) - Anti-ATIC antibody [EPR13243-53] (AB188321)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ATIC antibody [EPR13243-53] (AB188321)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa cells labeling ATIC with ab188321 at 1/140 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Supplier Data

Immunoprecipitation - Anti-ATIC antibody [EPR13243-53] (AB188321)

Western blot analysis of ATIC in Jurkat cell lysate immunoprecipitated with ab188321 at 1/50 dilution (Lane 1). Lane 2 : PBS instead of Jurkat lysate.

Secondary antibody : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

All lanes:

Immunoprecipitation - Anti-ATIC antibody [EPR13243-53] (ab188321)

Predicted band size: 65 kDa

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Supplier Data

Western blot - Anti-ATIC antibody [EPR13243-53] (AB188321)

All lanes:

Western blot - Anti-ATIC antibody [EPR13243-53] (ab188321) at 1/10000 dilution

Lane 1:

Jurkat cell lysate at 20 µg

Lane 2:

HeLa cell lysate at 20 µg

Lane 3:

HepG2 cell lysate at 20 µg

Lane 4:

HCT-116 cell lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution

Predicted band size: 65 kDa

Observed band size: 65 kDa

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Carrier free

Anti-ATIC antibody [EPR13243-53] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13243-53

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, Flow Cyt (Intra), IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/40 - 1/60", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/140", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." } } }

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Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Conditional Ambient

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATIC also known as AICAR transformylase/IMP cyclohydrolase is a bifunctional enzyme involved in purine biosynthesis within cells. It has a molecular mass of approximately 64 kDa. This enzyme is found in various tissues including liver kidney and brain. It catalyzes the last two steps in the de novo purine biosynthesis pathway playing an important role in cellular proliferation and energy homeostasis.

Biological function summary

ATIC participates importantly in purine metabolism by facilitating the conversion of 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) into inosine monophosphate (IMP). It acts both as a transformylase and cyclohydrolase forming part of the multi-enzyme purinosome complex. This involvement ensures efficient synthesis of IMP which is a precursor for adenosine and guanosine nucleotide production essential for DNA and RNA synthesis.

Pathways

ATIC is integral to the de novo purine biosynthesis pathway a critical cellular process for generating nucleotides. It interacts with other enzymes in this pathway such as phosphoribosylaminoimidazole carboxamide formyltransferase (PAIC) and adenylosuccinate lyase (ADSL) ensuring a steady supply of nucleotides. This pathway is also linked to the folate cycle highlighting the importance of ATIC in broader metabolic contexts.

ATIC has associations with several conditions notably cancer and autoimmune diseases. Its role in purine and folate metabolism makes it a target in cancer therapies where fast-dividing cells depend on nucleotide synthesis. ATIC also connects to dihydrofolate reductase (DHFR) due to the shared pathway implicating both in therapies aimed at autoimmune conditions like rheumatoid arthritis. Understanding ATIC's function and interaction plays a part in advancing disease treatment strategies.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Bifunctional enzyme that catalyzes the last two steps of purine biosynthesis (PubMed : 11948179, PubMed : 14756554). Acts as a transformylase that incorporates a formyl group to the AMP analog AICAR (5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide) to produce the intermediate formyl-AICAR (FAICAR) (PubMed : 10985775, PubMed : 11948179, PubMed : 9378707). Can use both 10-formyldihydrofolate and 10-formyltetrahydrofolate as the formyl donor in this reaction (PubMed : 10985775). Also catalyzes the cyclization of FAICAR to inosine monophosphate (IMP) (PubMed : 11948179, PubMed : 14756554). Is able to convert thio-AICAR to 6-mercaptopurine ribonucleotide, an inhibitor of purine biosynthesis used in the treatment of human leukemias (PubMed : 10985775). Promotes insulin receptor/INSR autophosphorylation and is involved in INSR internalization (PubMed : 25687571).

See full target information ATIC

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Publications (2)

Recent publications for all applications. Explore the full list and refine your search

Cell death & disease 14:87 PubMed36750554

2023

p53 promotes peroxisomal fatty acid β-oxidation to repress purine biosynthesis and mediate tumor suppression.

Applications

Unspecified application

Species

Unspecified reactive species

Jianhong Zhao,Xiaojun Zhou,Baoxiang Chen,Mingzhu Lu,Genxin Wang,Nagarajan Elumalai,Chenhui Tian,Jinmiao Zhang,Yanliang Liu,Zhiqiang Chen,Xinyi Zhou,Mingzhi Wu,Mengjiao Li,Edward V Prochownik,Ali Tavassoli,Congqing Jiang,Youjun Li

Cell death & disease 10:627 PubMed31427568

2019

Deletion of OSBPL2 in auditory cells increases cholesterol biosynthesis and drives reactive oxygen species production by inhibiting AMPK activity.

Applications

Unspecified application

Species

Unspecified reactive species

Hongshun Wang,Changsong Lin,Jun Yao,Hairong Shi,Cui Zhang,Qinjun Wei,Yajie Lu,Zhibin Chen,Guangqian Xing,Xin Cao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATIC antibody [EPR13243-53]

Flow Cytometry (Intracellular) - Anti-ATIC antibody [EPR13243-53] (AB188321)

Immunoprecipitation - Anti-ATIC antibody [EPR13243-53] (AB188321)

Western blot - Anti-ATIC antibody [EPR13243-53] (AB188321)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

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Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (2)

Product promise