JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB232532

Anti-ATIC antibody [EPR13243-53] - BSA and Azide free

Be the first to review this product! Submit a review

|

(1 Publication)

Rabbit Recombinant Monoclonal ATIC antibody. Carrier free. Suitable for IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

PURH, OK/SW-cl.86, ATIC, Bifunctional purine biosynthesis protein ATIC, AICAR transformylase/inosine monophosphate cyclohydrolase

2 Images
Flow Cytometry (Intracellular) - Anti-ATIC antibody [EPR13243-53] - BSA and Azide free (AB232532)
  • Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-ATIC antibody [EPR13243-53] - BSA and Azide free (AB232532)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling ATIC with ab188321 at 1/140 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188321).

Immunoprecipitation - Anti-ATIC antibody [EPR13243-53] - BSA and Azide free (AB232532)
  • IP

Supplier Data

Immunoprecipitation - Anti-ATIC antibody [EPR13243-53] - BSA and Azide free (AB232532)

Western blot analysis of ATIC in Jurkat cell lysate immunoprecipitated with ab188321 at 1/50 dilution (Lane 1). Lane 2 : PBS instead of Jurkat lysate.

Secondary antibody : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab188321).

All lanes:

Immunoprecipitation - Anti-ATIC antibody [EPR13243-53] (<a href='/en-us/products/primary-antibodies/atic-antibody-epr13243-53-ab188321'>ab188321</a>)

Predicted band size: 65 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR13243-53

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Alternative Products

Primary Antibodies

AB33520

Anti-ATIC antibody [F38 P7 H9]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATIC antibody [F38 P7 H9] (AB33520)

  • 4
  • 1

Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB8227

Anti-beta Actin antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Western blot - Anti-beta Actin antibody - Loading Control (AB8227)

  • 5
  • 104
Primary Antibodies

AB186130

Anti-SHMT1 antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHMT1 antibody (AB186130)

  • 0
  • 0
Primary Antibodies

AB32072

Anti-c-Myc antibody [Y69] - ChIP Grade

BOND RX™ Validated|KO Validated|RabMAb|Recombinant|Lab Essentials|20ul selling size|Advanced Validation

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-c-Myc antibody [Y69] - ChIP Grade (AB32072)

  • 4
  • 56
style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p><a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.</p>" } } }
style
left-column

Product details

ab232532 is the carrier-free version of ab188321.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATIC also known as AICAR transformylase/IMP cyclohydrolase is a bifunctional enzyme involved in purine biosynthesis within cells. It has a molecular mass of approximately 64 kDa. This enzyme is found in various tissues including liver kidney and brain. It catalyzes the last two steps in the de novo purine biosynthesis pathway playing an important role in cellular proliferation and energy homeostasis.
Biological function summary

ATIC participates importantly in purine metabolism by facilitating the conversion of 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR) into inosine monophosphate (IMP). It acts both as a transformylase and cyclohydrolase forming part of the multi-enzyme purinosome complex. This involvement ensures efficient synthesis of IMP which is a precursor for adenosine and guanosine nucleotide production essential for DNA and RNA synthesis.

Pathways

ATIC is integral to the de novo purine biosynthesis pathway a critical cellular process for generating nucleotides. It interacts with other enzymes in this pathway such as phosphoribosylaminoimidazole carboxamide formyltransferase (PAIC) and adenylosuccinate lyase (ADSL) ensuring a steady supply of nucleotides. This pathway is also linked to the folate cycle highlighting the importance of ATIC in broader metabolic contexts.

ATIC has associations with several conditions notably cancer and autoimmune diseases. Its role in purine and folate metabolism makes it a target in cancer therapies where fast-dividing cells depend on nucleotide synthesis. ATIC also connects to dihydrofolate reductase (DHFR) due to the shared pathway implicating both in therapies aimed at autoimmune conditions like rheumatoid arthritis. Understanding ATIC's function and interaction plays a part in advancing disease treatment strategies.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Bifunctional enzyme that catalyzes the last two steps of purine biosynthesis (PubMed : 11948179, PubMed : 14756554). Acts as a transformylase that incorporates a formyl group to the AMP analog AICAR (5-amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide) to produce the intermediate formyl-AICAR (FAICAR) (PubMed : 10985775, PubMed : 11948179, PubMed : 9378707). Can use both 10-formyldihydrofolate and 10-formyltetrahydrofolate as the formyl donor in this reaction (PubMed : 10985775). Also catalyzes the cyclization of FAICAR to inosine monophosphate (IMP) (PubMed : 11948179, PubMed : 14756554). Is able to convert thio-AICAR to 6-mercaptopurine ribonucleotide, an inhibitor of purine biosynthesis used in the treatment of human leukemias (PubMed : 10985775). Promotes insulin receptor/INSR autophosphorylation and is involved in INSR internalization (PubMed : 25687571).
See full target information ATIC
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Glia 72:2061-2078 PubMed39056459

2024

Astrocyte-TREM2 alleviates brain injury by regulating reactive astrocyte states following ischemic stroke.

Applications

Unspecified application

Species

Unspecified reactive species

Cong Wang,Jing Dong,Heng Huang,Kegui Zhou,Zhenguo Liu,Richard Milner,Longxuan Li
View all publications
style
left-column
style
left-column
style
right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com