AB117819

Anti-ATL3 antibody

Name: Anti-ATL3 antibody (ab117819) | Abcam
Brand: Abcam Limited
SKU: AB117819
Rating: 5 (1 reviews)

(1 Review)

(5 Publications)

Rabbit Polyclonal ATL3 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 5 publications. Immunogen corresponding to Synthetic Peptide within Human Atlastin-3 aa 350-400.

View Alternative Names

Atlastin-3, ATL3

2 Images

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Immunoprecipitation - Anti-ATL3 antibody (AB117819)

Detection of Human ATL3 by Western Blot of Immunoprecipitation.
ab117819 at 1µg/ml staining ATL3 in HeLa whole cell lysate immunoprecipitated using ab117819 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection : Chemiluminescence with exposure time of 10 seconds.

All lanes:

Immunoprecipitation - Anti-ATL3 antibody (ab117819)

Predicted band size: 61 kDa

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Unknown

Western blot - Anti-ATL3 antibody (AB117819)

All lanes:

Western blot - Anti-ATL3 antibody (ab117819) at 0.04 µg/mL

Lane 1:

HeLa whole cell lysate at 50 µg

Lane 2:

HeLa whole cell lysate at 15 µg

Lane 3:

Jurkat whole cell lysate at 50 µg

Lane 4:

293T whole cell lysate at 50 µg

Lane 5:

NIH3T3 whole cell lysate at 50 µg

Predicted band size: 61 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human Atlastin-3 aa 350-400. The exact immunogen used to generate this antibody is proprietary information.

Q6DD88

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "2-10 µg/mg of lysate", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000 - 1/10000", "WB-species-notes": "" }, "Rat": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chinese hamster": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Orangutan": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "IP-species-checked": "predicted", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 6.8 - 7.4 Preservative: 0.09% Sodium azide Constituents: 99% Tris buffered saline, 0.1% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ATL3 protein also known as Atlastin GTPase 3 functions mechanically in cellular processes like membrane fusion. It belongs to the family of dynamin-like GTPases characterized by their ability to hydrolyze GTP to fuel various cellular activities. ATL3 has a molecular mass of about 61 kDa. Expression of ATL3 occurs in various tissues with a significant presence in the brain possibly hinting at its specific roles in neurological functions.

Biological function summary

ATL3 participates in the maintenance of the tubular network of the endoplasmic reticulum (ER). The protein often acts as a dimer and works similarly to other members of the atlastin family. This dimerization and its involvement in membrane fusion aid in creating and maintaining the three-dimensional structure of the ER which is important for various cellular functions and signaling pathways. ATL3 also contributes to the proper spatial arrangement of organelles inside the cells.

Pathways

ATL3 is actively involved in the endoplasmic reticulum morphogenesis pathways. The protein plays an important role in shaping the ER which impacts lipid metabolism and protein synthesis pathways. It closely interacts with other ER-shaping proteins such as ATL1 and ATL2 assisting in retaining the three-dimensional integrity of the ER network that is essential for efficient cellular functioning and response to stress.

Mutations or malfunctions in ATL3 can be associated with hereditary sensory and autonomic neuropathy type I. This relation highlights the significance of ATL3 in neuronal health potentially because of its influence on the ER's structure that affects neuron survival. Additionally there is a connection between ATL3 dysfunction and hereditary spastic paraplegia with ATL1 as another implicated protein indicating a broader impact on neurodegenerative conditions. Understanding ATL3's role not only clarifies its function but also offers insight into potential therapeutic targets for related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

GTPase tethering membranes through formation of trans-homooligomers and mediating homotypic fusion of endoplasmic reticulum membranes. Functions in endoplasmic reticulum tubular network biogenesis (PubMed : 18270207, PubMed : 19665976, PubMed : 27619977).

See full target information Atlastin-3

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

mBio 15:e0281124 PubMed39431827

2024

Rab6a enables BICD2/dynein-mediated trafficking of human papillomavirus from the Golgi network during virus entry.

Applications

Unspecified application

Species

Unspecified reactive species

Jeongjoon Choi,Kaitlyn Speckhart,Billy Tsai,Daniel DiMaio

Autophagy 20:2067-2075 PubMed38818751

2024

RTN3L and CALCOCO1 function in parallel to maintain proteostasis in the endoplasmic reticulum.

Applications

Unspecified application

Species

Unspecified reactive species

Kamal Kumar,Ravi Chidambaram,Smriti Parashar,Susan Ferro-Novick

The Journal of cell biology 222: PubMed37102997

2023

Human atlastin-3 is a constitutive ER membrane fusion catalyst.

Applications

Unspecified application

Species

Unspecified reactive species

Samantha Bryce,Maureen Stolzer,Daniel Crosby,Ruijin Yang,Dannie Durand,Tina H Lee

The Journal of cell biology 222: PubMed36757370

2023

Human atlastins are sufficient to drive the fusion of liposomes with a physiological lipid composition.

Applications

Unspecified application

Species

Unspecified reactive species

Eunhong Jang,Yeojin Moon,So Young Yoon,Joyce Anne R Diaz,Miriam Lee,Naho Ko,Jongseo Park,Soo Hyun Eom,Changwook Lee,Youngsoo Jun

The Journal of cell biology 220: PubMed33988678

2021

Atlastin 2/3 regulate ER targeting of the ULK1 complex to initiate autophagy.

Applications

Unspecified application

Species

Unspecified reactive species

Nan Liu,Hongyu Zhao,Yan G Zhao,Junjie Hu,Hong Zhang

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATL3 antibody

Immunoprecipitation - Anti-ATL3 antibody (AB117819)

Western blot - Anti-ATL3 antibody (AB117819)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

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