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AB229095

Anti-ATL3 antibody

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(1 Publication)

Rabbit Polyclonal ATL3 antibody. Suitable for WB, IHC-P and reacts with Human, Rat samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human Atlastin-3.

View Alternative Names

Atlastin-3, AT3, ATL-3, ATL3

4 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATL3 antibody (AB229095)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATL3 antibody (AB229095)

Paraffin-embedded rat liver tissue stained for ATL3 protein using ab229095 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATL3 antibody (AB229095)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATL3 antibody (AB229095)

Paraffin-embedded rat brain tissue stained for ATL3 protein using ab229095 at 1/500 dilution in immunohistochemical analysis.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATL3 antibody (AB229095)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATL3 antibody (AB229095)

Paraffin-embedded rat cervix tissue stained for ATL3 protein using ab229095 at 1/500 dilution in immunohistochemical analysis.

Western blot - Anti-ATL3 antibody (AB229095)
  • WB

Supplier Data

Western blot - Anti-ATL3 antibody (AB229095)

7.5 % SDS-PAGE gel.

All lanes:

Western blot - Anti-ATL3 antibody (ab229095) at 1/1000 dilution

All lanes:

NT2/D1 whole cell lysate at 30 µg

Predicted band size: 61 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Rat, Human

Applications

IHC-P, WB

applications

Immunogen

Recombinant Fragment Protein within Human Atlastin-3. The exact immunogen used to generate this antibody is proprietary information.

Q6DD88

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/3000", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Rat": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/100 - 1/1000", "IHCP-species-notes": "<p></p>" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7 Preservative: 0.01% Thimerosal (merthiolate) Constituents: PBS, 20% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The ATL3 protein also known as Atlastin GTPase 3 functions mechanically in cellular processes like membrane fusion. It belongs to the family of dynamin-like GTPases characterized by their ability to hydrolyze GTP to fuel various cellular activities. ATL3 has a molecular mass of about 61 kDa. Expression of ATL3 occurs in various tissues with a significant presence in the brain possibly hinting at its specific roles in neurological functions.
Biological function summary

ATL3 participates in the maintenance of the tubular network of the endoplasmic reticulum (ER). The protein often acts as a dimer and works similarly to other members of the atlastin family. This dimerization and its involvement in membrane fusion aid in creating and maintaining the three-dimensional structure of the ER which is important for various cellular functions and signaling pathways. ATL3 also contributes to the proper spatial arrangement of organelles inside the cells.

Pathways

ATL3 is actively involved in the endoplasmic reticulum morphogenesis pathways. The protein plays an important role in shaping the ER which impacts lipid metabolism and protein synthesis pathways. It closely interacts with other ER-shaping proteins such as ATL1 and ATL2 assisting in retaining the three-dimensional integrity of the ER network that is essential for efficient cellular functioning and response to stress.

Mutations or malfunctions in ATL3 can be associated with hereditary sensory and autonomic neuropathy type I. This relation highlights the significance of ATL3 in neuronal health potentially because of its influence on the ER's structure that affects neuron survival. Additionally there is a connection between ATL3 dysfunction and hereditary spastic paraplegia with ATL1 as another implicated protein indicating a broader impact on neurodegenerative conditions. Understanding ATL3's role not only clarifies its function but also offers insight into potential therapeutic targets for related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Atlastin-3 (ATL3) is a membrane-anchored GTPase that mediates the GTP-dependent fusion of endoplasmic reticulum (ER) membranes, maintaining the continuous ER network. It facilitates the formation of three-way junctions where ER tubules intersect (PubMed : 18270207, PubMed : 19665976, PubMed : 24459106, PubMed : 27619977, PubMed : 37102997). Two atlastin-3 on neighboring ER tubules bind GTP and form loose homodimers through the GB1/RHD3-type G domains and 3HB regions. Upon GTP hydrolysis, the 3HB regions tighten, pulling the membranes together to drive their fusion. After fusion, the homodimer disassembles upon release of inorganic phosphate (Pi). Subsequently, GDP dissociates, resetting the monomers to a conformation ready for a new fusion cycle (By similarity).
See full target information Atlastin-3
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 297:101307 PubMed34662580

2021

Neddylation modification of the U3 snoRNA-binding protein RRP9 by Smurf1 promotes tumorigenesis.

Applications

Unspecified application

Species

Unspecified reactive species

Meng-Ge Du,Fan Liu,Yan Chang,Shuai Tong,Wei Liu,Yu-Jiao Chen,Ping Xie
View all publications
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Product promise

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