Rabbit Polyclonal ATM antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human ATM aa 2550-2600.
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- Oncology reports 45:2021MicroRNA‑129 inhibits colorectal cancer cell proliferation, invasion and epithelial‑to‑mesenchymal transition by targeting SOX4.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZhiping Chen,Tianyu Zhong,Jinghua Zhong,Yang Tang,Baodian Ling,Lanfeng WangPubMed 33760159
- Molecular cancer therapeutics 18:2283-22952019CNDAC-Induced DNA Double-Strand Breaks Cause Aberrant Mitosis Prior to Cell Death.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaojun Liu et. al.PubMed 31501277
- Cell death and differentiation 26:812-8252018Regulating BRCA1 protein stability by cathepsin S-mediated ubiquitin degradation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSeoYoung Kim et. al.PubMed 30006610
- Journal of cell science 131:2018Overexpression of PP1-NIPP1 limits the capacity of cells to repair DNA double-strand breaks.Applications:Unspecified applicationReactive species:Unspecified reactive speciesClaudia Winkler et. al.PubMed 29898919
- Oncology letters 16:2135-21422018Hint1 expression inhibits proliferation and promotes radiosensitivity of human SGC7901 gastric cancer cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaowei Wei et. al.PubMed 30008911
- Oncogenesis 5:e2802016The spliceosome U2 snRNP factors promote genome stability through distinct mechanisms; transcription of repair factors and R-loop processing.
- The Journal of biological chemistry 291:25516-255282016URI Regulates KAP1 Phosphorylation and Transcriptional Repression via PP2A Phosphatase in Prostate Cancer Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesPaolo Mita et. al.PubMed 27780869
- Oncotarget 8:22104-221122016A phase I-II trial of fludarabine, bendamustine and rituximab (FBR) in previously treated patients with CLL.Applications:WBReactive species:HumanNitin Jain,Kumudha Balakrishnan,Alessandra Ferrajoli,Susan M O'Brien,Jan A Burger,Tapan M Kadia,Jorge E Cortes,Mary L Ayres,Francesco Paolo Tambaro,Michael J Keating,Varsha Gandhi,William G WierdaPubMed 27655665
- Orphanet journal of rare diseases 8:1232013SETX mutations are a frequent genetic cause of juvenile and adult onset cerebellar ataxia with neuropathy and elevated serum alpha-fetoprotein.Applications:WBReactive species:HumanLorenzo Nanetti,Simona Cavalieri,Viviana Pensato,Alessandra Erbetta,Davide Pareyson,Marta Panzeri,Giovanna Zorzi,Carlo Antozzi,Isabella Moroni,Cinzia Gellera,Alfredo Brusco,Caterina MariottiPubMed 23941260
- European journal of human genetics : EJHG 21:774-82012Deep-intronic ATM mutation detected by genomic resequencing and corrected in vitro by antisense morpholino oligonucleotide (AMO).Applications:Unspecified applicationReactive species:Unspecified reactive speciesSimona Cavalieri,Elisa Pozzi,Richard A Gatti,Alfredo BruscoPubMed 23211698
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human ATM aa 2550-2600. The exact immunogen used to generate this antibody is proprietary information. Database link Q13315
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Reactivity data
Select an application| WB | IHC-P | |
|---|---|---|
| Human | Tested | Tested |
| Chimpanzee | Predicted | Predicted |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/5000 - 1/25000 | Notes Do not perform antigen retrieval. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500 - 1/2000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Chimpanzee | Dilution info - | Notes - |
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Target data
Function
Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates DYRK2, CHEK2, p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, UFL1, RAD9, UBQLN4 and DCLRE1C (PubMed:9843217, PubMed:9733515, PubMed:10550055, PubMed:10766245, PubMed:10839545, PubMed:10910365, PubMed:10802669, PubMed:10973490, PubMed:11375976, PubMed:12086603, PubMed:15456891, PubMed:19965871, PubMed:30612738, PubMed:30886146). May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends. Phosphorylation of DYRK2 in nucleus in response to genotoxic stress prevents its MDM2-mediated ubiquitination and subsequent proteasome degradation. Phosphorylates ATF2 which stimulates its function in DNA damage response. Phosphorylates ERCC6 which is essential for its chromatin remodeling activity at DNA double-strand breaks (PubMed:29203878).
Alternative names
Serine-protein kinase ATM, Ataxia telangiectasia mutated, A-T mutated, ATM
Recommended products
Rabbit Polyclonal ATM antibody. Suitable for WB, IHC-P and reacts with Human samples. Cited in 15 publications. Immunogen corresponding to Synthetic Peptide within Human ATM aa 2550-2600.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human ATM aa 2550-2600. The exact immunogen used to generate this antibody is proprietary information. Database link Q13315
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
Antibody was affinity purified using an epitope specific to ATM immobilized on solid support.
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
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Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ATM also known as Ataxia Telangiectasia Mutated is a protein kinase with a molecular weight of approximately 370 kDa. ATM protein primarily resides in the cell nucleus and functions as a critical regulator of the cell cycle. It plays a significant role in the detection of DNA damage and initiation of repair processes. As part of its mechanical functions ATM phosphorylates serine and threonine residues on various substrates most notably in response to double-strand breaks in DNA. This activity is important for maintaining genomic stability.
- Biological function summary
ATM acts as a coordinator in cellular response to DNA damage highly interacting with multiple components of the DNA repair machinery. It forms a complex with proteins like NBS1 and MRN complex facilitating repair by recruiting and activating other proteins involved in homologous recombination and non-homologous end joining pathways. ATM also modulates p53 activity a primary response factor in cellular stress management linking ATM to control of cell cycle arrest and apoptosis. This positions ATM as an integral part of maintaining cellular integrity in face of genomic insult.
- Pathways
ATM integrates neatly within the DNA damage response and cell cycle control pathways. ATM's operative relationship with the MRN complex and its role in the PI3K-related protein kinase family helps initiate appropriate repair processes upon DNA damage detection. Additionally ATM regulates the activity of proteins such as Chk2 which further propagates signals to p53 influencing decisions between cell cycle arrest and apoptosis. These interactions link ATM closely to essential processes like DNA repair and cell survival highlighting its role in genomic maintenance.
- Associated diseases and disorders
ATM mutations or dysregulation leads to Ataxia Telangiectasia an autosomal recessive disorder characterized by neurodegeneration immune deficiencies and cancer predisposition. ATM dysfunction also connects to cancer development particularly breast cancer where it transmits signals involving BRCA1 contributing to DNA repair through homologous recombination. Understanding ATM dynamics and related pathways has important implications for developing therapeutic strategies to manage or mitigate effects associated with its dysfunction.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM antibody (ab17995)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate-nodular hypertrophy tissue labelling ATM with ab17995 at 1/1000 (1µg/ml). Detection: DAB.
Western blot - Anti-ATM antibody (ab17995)
Detection of Human ATM using ab17995 by Western Blot and Immunoprecipitation. Samples: A) Whole cell lysate (50 μg) from human L-40 (WT) or AT-59 (AT) cells. B) Whole cell lysate (1 mg) from WT cells. Antibody used at indicated concentrations for WB and at 0.5 μg/mg lysate for IP. Immunoprecipitated ATM was blotted with a monoclonal antibody to ATM. Detected by chemiluminescence with an exposure time of 30 seconds.
All lanes: Western blot - Anti-ATM antibody (ab17995)
Predicted band size: 351 kDa
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