Rabbit Recombinant Monoclonal ATM antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 22 publications.
Images
![Western blot - Anti-ATM antibody [EPR17059] (AB199726), expandable thumbnail](https://content.abcam.com/products/images/atm-antibody-epr17059-ab199726--western-blot-img170116.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ATM antibody [EPR17059] (AB199726)")
![Immunoprecipitation - Anti-ATM antibody [EPR17059] (AB199726), expandable thumbnail](https://content.abcam.com/products/images/atm-antibody-epr17059-ab199726--immunoprecipitation-img1935.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-ATM antibody [EPR17059] (AB199726)")
![Western blot - Anti-ATM antibody [EPR17059] (AB199726), expandable thumbnail](https://content.abcam.com/products/images/atm-antibody-epr17059-ab199726--western-blot-img23700.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ATM antibody [EPR17059] (AB199726)")
![Immunoprecipitation - Anti-ATM antibody [EPR17059] (AB199726), expandable thumbnail](https://content.abcam.com/products/images/atm-antibody-epr17059-ab199726--immunoprecipitation-img47207.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-ATM antibody [EPR17059] (AB199726)")
![Western blot - Anti-ATM antibody [EPR17059] (AB199726), expandable thumbnail](https://content.abcam.com/products/images/atm-antibody-epr17059-ab199726--western-blot-img55330.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ATM antibody [EPR17059] (AB199726)")
Publications
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- Cancer & metabolism 11:202023ATM inhibition blocks glucose metabolism and amplifies the sensitivity of resistant lung cancer cell lines to oncogene driver inhibitors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCristina Terlizzi,Viviana De Rosa,Francesca Iommelli,Antonio Pezone,Giovanna G Altobelli,Maurizio Maddalena,Jelena Dimitrov,Caterina De Rosa,Carminia Maria Della Corte,Vittorio Enrico Avvedimento,Silvana Del VecchioPubMed 37932830
- Acta biochimica et biophysica Sinica 55:842-8522023The FBXO32/ATR/ATM axis acts as a molecular switch to control the sensitivity of osteosarcoma cells to irradiation through its regulation of EXO1 expression.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYao Lu,Panpan Huang,Yanli Li,Wenyu Liu,Jing Li,Rui Zhao,Haihua Feng,Ce Shi,Gaolu CaoPubMed 37227155
- Nature communications 14:12442023Loss of p53 activates thyroid hormone via type 2 deiodinase and enhances DNA damage.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAnnarita Nappi,Caterina Miro,Antonio Pezone,Alfonso Tramontano,Emery Di Cicco,Serena Sagliocchi,Annunziata Gaetana Cicatiello,Melania Murolo,Sepehr Torabinejad,Elena Abbotto,Giuseppina Caiazzo,Maddalena Raia,Mariano Stornaiuolo,Dario Antonini,Gabriella Fabbrocini,Domenico Salvatore,Vittorio Enrico Avvedimento,Monica DenticePubMed 36871014
- Aging 15:492-5122023Selective ATM inhibition augments radiation-induced inflammatory signaling and cancer cell death.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi-Ya Chiu,Qing Sun,Frank T Zenke,Andree Blaukat,Lyubomir T VassilevPubMed 36656721
- Frontiers in nutrition 9:8546552022Folic Acid Preconditioning Alleviated Radiation-Induced Ovarian Dysfunction in Female Mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesQianyu Zhang,Zhifu Wei,Huinan Weng,Ye Chen,Jie Zhang,Shiwei Mei,Jiahui Wei,Xiulan Zhu,Yingqi Nong,Jianxing Ruan,Wenjuan Liu,Ruiqiong Zhou,Fang Wang,Yanni Xie,Junjiu Huang,Xiqian Zhang,Fenghua LiuPubMed 35836584
- Oxidative medicine and cellular longevity 2022:42012872022Inhibition of PLK3 Attenuates Tubular Epithelial Cell Apoptosis after Renal Ischemia-Reperfusion Injury by Blocking the ATM/P53-Mediated DNA Damage Response.Applications:Unspecified applicationReactive species:Unspecified reactive speciesWeiming Deng,Xiangling Wei,Zhenwei Xie,Rui Zhang,Zhanwen Dong,Jinhua Zhang,You Luo,Qingdi Cheng,Ruojiao Wang,Heng Li,Ning NaPubMed 35783188
- Molecular cancer therapeutics 21:859-8702022A New Class of Selective ATM Inhibitors as Combination Partners of DNA Double-Strand Break Inducing Cancer Therapies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAstrid Zimmermann,Frank T Zenke,Li-Ya Chiu,Heike Dahmen,Ulrich Pehl,Thomas Fuchss,Thomas Grombacher,Beatrix Blume,Lyubomir T Vassilev,Andree BlaukatPubMed 35405736
- Acta biochimica et biophysica Sinica 54:37-462022miR-1205/DNAJB1 reverses docetaxel chemoresistance in human triple negative breast carcinoma cells via regulation of mutp53/TAp63 signaling.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYongxiang Yin,Jinqiu Zhang,Tao Ma,Daozhen Chen,Daru LuPubMed 35130632
- Osteoporosis international : a journal established as result of cooperation between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA 33:453-4662021Exogenous melatonin prevents type 1 diabetes mellitus-induced bone loss, probably by inhibiting senescence.Applications:Unspecified applicationReactive species:Unspecified reactive speciesZ Gong,W Da,Y Tian,R Zhao,S Qiu,Q Wu,K Wen,L Shen,R Zhou,L Tao,Y ZhuPubMed 34519833
- PloS one 16:e02535332021The genetic association of the transcription factor NPAT with glycemic response to metformin involves regulation of fuel selection.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChangwei Chen,Jennifer R Gallagher,Jamie Tarlton,Lidy van Aalten,Susan E Bray,Michael L J Ashford,Rory J McCrimmon,Ewan R Pearson,Alison D McNeilly,Calum SutherlandPubMed 34197485
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|
| Human | Tested | Not recommended | Tested | Not recommended |
| Mouse | Expected | Not recommended | Tested | Not recommended |
| Rat | Expected | Not recommended | Tested | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100 | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 1/2000 | Notes - |
Species Rat | Dilution info 1/2000 | Notes - |
Species Human | Dilution info 1/2000 | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
Target data
Function
Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor (PubMed:10550055, PubMed:10839545, PubMed:10910365, PubMed:12556884, PubMed:14871926, PubMed:15064416, PubMed:15448695, PubMed:15456891, PubMed:15790808, PubMed:15916964, PubMed:17923702, PubMed:21757780, PubMed:24534091, PubMed:35076389, PubMed:9733514). Recognizes the substrate consensus sequence [ST]-Q (PubMed:10550055, PubMed:10839545, PubMed:10910365, PubMed:12556884, PubMed:14871926, PubMed:15448695, PubMed:15456891, PubMed:15916964, PubMed:17923702, PubMed:24534091, PubMed:9733514). Phosphorylates 'Ser-139' of histone variant H2AX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism (By similarity). Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates DYRK2, CHEK2, p53/TP53, FBXW7, FANCD2, NFKBIA, BRCA1, CREBBP/CBP, RBBP8/CTIP, MRE11, nibrin (NBN), RAD50, RAD17, PELI1, TERF1, UFL1, RAD9, UBQLN4 and DCLRE1C (PubMed:10550055, PubMed:10766245, PubMed:10802669, PubMed:10839545, PubMed:10910365, PubMed:10973490, PubMed:11375976, PubMed:12086603, PubMed:15456891, PubMed:19965871, PubMed:21757780, PubMed:24534091, PubMed:26240375, PubMed:26774286, PubMed:30612738, PubMed:30886146, PubMed:30952868, PubMed:38128537, PubMed:9733515, PubMed:9843217). May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends. Phosphorylation of DYRK2 in nucleus in response to genotoxic stress prevents its MDM2-mediated ubiquitination and subsequent proteasome degradation (PubMed:19965871). Phosphorylates ATF2 which stimulates its function in DNA damage response (PubMed:15916964). Phosphorylates ERCC6 which is essential for its chromatin remodeling activity at DNA double-strand breaks (PubMed:29203878). Phosphorylates TTC5/STRAP at 'Ser-203' in the cytoplasm in response to DNA damage, which promotes TTC5/STRAP nuclear localization (PubMed:15448695). Also involved in pexophagy by mediating phosphorylation of PEX5: translocated to peroxisomes in response to reactive oxygen species (ROS), and catalyzes phosphorylation of PEX5, promoting PEX5 ubiquitination and induction of pexophagy (PubMed:26344566).
Alternative names
Serine-protein kinase ATM, Ataxia telangiectasia mutated, A-T mutated, ATM
Recommended products
Rabbit Recombinant Monoclonal ATM antibody. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 22 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR17059
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ATM also known as Ataxia Telangiectasia Mutated is a protein kinase with a molecular weight of approximately 370 kDa. ATM protein primarily resides in the cell nucleus and functions as a critical regulator of the cell cycle. It plays a significant role in the detection of DNA damage and initiation of repair processes. As part of its mechanical functions ATM phosphorylates serine and threonine residues on various substrates most notably in response to double-strand breaks in DNA. This activity is important for maintaining genomic stability.
- Biological function summary
ATM acts as a coordinator in cellular response to DNA damage highly interacting with multiple components of the DNA repair machinery. It forms a complex with proteins like NBS1 and MRN complex facilitating repair by recruiting and activating other proteins involved in homologous recombination and non-homologous end joining pathways. ATM also modulates p53 activity a primary response factor in cellular stress management linking ATM to control of cell cycle arrest and apoptosis. This positions ATM as an integral part of maintaining cellular integrity in face of genomic insult.
- Pathways
ATM integrates neatly within the DNA damage response and cell cycle control pathways. ATM's operative relationship with the MRN complex and its role in the PI3K-related protein kinase family helps initiate appropriate repair processes upon DNA damage detection. Additionally ATM regulates the activity of proteins such as Chk2 which further propagates signals to p53 influencing decisions between cell cycle arrest and apoptosis. These interactions link ATM closely to essential processes like DNA repair and cell survival highlighting its role in genomic maintenance.
- Associated diseases and disorders
ATM mutations or dysregulation leads to Ataxia Telangiectasia an autosomal recessive disorder characterized by neurodegeneration immune deficiencies and cancer predisposition. ATM dysfunction also connects to cancer development particularly breast cancer where it transmits signals involving BRCA1 contributing to DNA repair through homologous recombination. Understanding ATM dynamics and related pathways has important implications for developing therapeutic strategies to manage or mitigate effects associated with its dysfunction.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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8 product images
![Western blot - Anti-ATM antibody [EPR17059] (ab199726), expandable thumbnail](https://content.abcam.com/products/images/atm-antibody-epr17059-ab199726--western-blot-img170116.jpg "Western blot - Anti-ATM antibody [EPR17059] (ab199726)")
Western blot - Anti-ATM antibody [EPR17059] (ab199726)
False colour image of Western blot: Anti-ATM antibody [EPR17059] staining at 1/2000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (Anti-alpha Tubulin antibody [DM1A] - Loading Control ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab199726 was shown to bind specifically to ATM. A band was observed at 350 kDa in wild-type A549 cell lysates with no signal observed at this size in ATM knockout cell line Human ATM knockout A549 cell line ab276095 (knockout cell lysate ab283834). To generate this image, wild-type and ATM knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5% milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776) at 1/20000 dilution.
All lanes: Western blot - Anti-ATM antibody [EPR17059] (ab199726) at 1/2000 dilution
Lane 1: Wild-type A549 cell lysate at 20 µg
Lane 2: ATM knockout A549 cell lysate at 20 µg
Lane 2: Western blot - Human ATM knockout A549 cell line (Human ATM knockout A549 cell line ab276095)
Lane 3: HEK-293 cell lysate at 20 µg
Lane 4: U-2 OS cell lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 351 kDa
Observed band size: 350 kDa
![Immunoprecipitation - Anti-ATM antibody [EPR17059] (ab199726), expandable thumbnail](https://content.abcam.com/products/images/atm-antibody-epr17059-ab199726--immunoprecipitation-img1935.jpg "Immunoprecipitation - Anti-ATM antibody [EPR17059] (ab199726)")
Immunoprecipitation - Anti-ATM antibody [EPR17059] (ab199726)
ATM was immunoprecipitated from 1mg of HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate with ab199726 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab199726 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: HEK-293 whole cell lysate 10ug (Input). Lane 2: ab199726 IP in HEK-293 whole cell lysate. Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab199726 in HEK-293 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 10 seconds.
All lanes: Immunoprecipitation - Anti-ATM antibody [EPR17059] (ab199726)
Predicted band size: 351 kDa
![Western blot - Anti-ATM antibody [EPR17059] (ab199726), expandable thumbnail](https://content.abcam.com/products/images/atm-antibody-epr17059-ab199726--western-blot-img23700.jpg "Western blot - Anti-ATM antibody [EPR17059] (ab199726)")
Western blot - Anti-ATM antibody [EPR17059] (ab199726)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-ATM antibody [EPR17059] (ab199726) at 1/2000 dilution
Lane 1: Rat testis lysate at 10 µg
Lane 2: Mouse testis lysate at 10 µg
Lane 3: 293 (Human epithelial cells from embryonic kidney) whole cell lysate at 10 µg
Lane 4: SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 351 kDa
Observed band size: 350 kDa
Exposure time: 20s
![Immunoprecipitation - Anti-ATM antibody [EPR17059] (ab199726), expandable thumbnail](https://content.abcam.com/products/images/atm-antibody-epr17059-ab199726--immunoprecipitation-img47207.jpg "Immunoprecipitation - Anti-ATM antibody [EPR17059] (ab199726)")
Immunoprecipitation - Anti-ATM antibody [EPR17059] (ab199726)
ATM was immunoprecipitated from 1mg of HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate with ab199726 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab199726 at 1/500 dilution (Panel A) or Anti-ATM antibody [Y170] ab32420 at 1/500 dilution (Panel B). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: HEK-293 whole cell lysate 10ug (Input). Lane 2: ab199726 IP in HEK-293 whole cell lysate. Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab199726 in HEK-293 whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds (Panel A and B).All lanes: Immunoprecipitation - Anti-ATM antibody [EPR17059] (ab199726)
Predicted band size: 351 kDa
![Western blot - Anti-ATM antibody [EPR17059] (ab199726), expandable thumbnail](https://content.abcam.com/products/images/atm-antibody-epr17059-ab199726--western-blot-img55330.jpg "Western blot - Anti-ATM antibody [EPR17059] (ab199726)")
Western blot - Anti-ATM antibody [EPR17059] (ab199726)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-ATM antibody [EPR17059] (ab199726) at 1/2000 dilution
Lane 1: Rat testis lysate at 10 µg
Lane 2: Mouse testis lysate at 10 µg
Lane 3: Mouse spleen lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 351 kDa
Observed band size: 350 kDa
Exposure time: 3min
![Immunoprecipitation - Anti-ATM antibody [EPR17059] (ab199726), expandable thumbnail](https://content.abcam.com/products/images/atm-antibody-epr17059-ab199726--immunoprecipitation-img47205.jpg "Immunoprecipitation - Anti-ATM antibody [EPR17059] (ab199726)")
Immunoprecipitation - Anti-ATM antibody [EPR17059] (ab199726)
ATM was immunoprecipitated from 1mg of SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate with ab199726 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab199726 at 1/500 dilution (Panel A) or Anti-ATM antibody [Y170] ab32420 at 1/500 dilution (Panel B). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: SH-SY5Y whole cell lysate 10ug (Input). Lane 2: ab199726 IP in SH-SY5Y whole cell lysate. Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab199726 in SH-SY5Y whole cell lysate.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 30 seconds (Panel A and B).All lanes: Immunoprecipitation - Anti-ATM antibody [EPR17059] (ab199726)
Predicted band size: 351 kDa
![Western blot - Anti-ATM antibody [EPR17059] (ab199726), expandable thumbnail](https://content.abcam.com/products/images/atm-antibody-epr17059-ab199726--western-blot-img27747.jpg "Western blot - Anti-ATM antibody [EPR17059] (ab199726)")
Western blot - Anti-ATM antibody [EPR17059] (ab199726)
Blocking/Dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-ATM antibody [EPR17059] (ab199726) at 1/5000 dilution
All lanes: 293 (Human epithelial cells from embryonic kidney) whole cell lysate at 10 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 351 kDa
Observed band size: 350 kDa
Exposure time: 3min
![Western blot - Anti-ATM antibody [EPR17059] (ab199726), expandable thumbnail](https://content.abcam.com/products/images/atm-antibody-epr17059-ab199726--western-blot-img442798.jpg "Western blot - Anti-ATM antibody [EPR17059] (ab199726)")
Image collected and cropped by CiteAb under a CC-BY license from the publication
Western blot - Anti-ATM antibody [EPR17059] (ab199726)
ATM western blot using anti-ATM antibody [EPR17059] ab199726. Publication image and figure legend from Zhao, X., Guo, X., et al., 2018, Cell Death Dis, PubMed 29352124.
ab199726 was used in this publication in western blot. This may not be the same as the application(s) guaranteed by Abcam. For a full list of applications guaranteed by Abcam for ab199726 please see the product overview.
HBX specifically inhibits CHK2 phosphorylationa, b Western blot for γH2AX in control and HBX-expressing SUDHL-4 a, and DB b, cells with or without MTX treatment (4 ng/ml) for 48 h. c, d Western blot for DDR proteins (P-CHK2/CHK2/ATM and P-CHK1/CHK1) in control and HBX-expressing SUDHL-4 c, and DB d, cells with or without MTX treatment (4 ng/ml) for 48 h. e, f The mRNA levels of DDR proteins (ATM, CHK2, and CHK1) were largely unchanged in control and HBX-expressing SUDHL-4 e, and DB f, cells with or without MTX treatment. g,h The expression of the P-CHK2 downstream genes P53 and P21 in control and HBX-expressing SUDHL-4 g, and DB h, cells with or without MTX treatment. The results are shown as the mean ± SEM from triplicate experiments. GAPDH was used as a loading control
![Immunoprecipitation - Anti-ATM antibody [EPR17059] (ab199726), expandable thumbnail](https://content.abcam.com/products/images/atm-antibody-epr17059-ab199726--immunoprecipitation-img47205.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-ATM antibody [EPR17059] (ab199726)")
![Western blot - Anti-ATM antibody [EPR17059] (ab199726), expandable thumbnail](https://content.abcam.com/products/images/atm-antibody-epr17059-ab199726--western-blot-img27747.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ATM antibody [EPR17059] (ab199726)")
![Western blot - Anti-ATM antibody [EPR17059] (ab199726), expandable thumbnail](https://content.abcam.com/products/images/atm-antibody-epr17059-ab199726--western-blot-img442798.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ATM antibody [EPR17059] (ab199726)")
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