AB232588

Anti-ATM antibody [EPR17059] - BSA and Azide free

RabMAb
Recombinant
KO Validated
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(1 Publication)

Rabbit Recombinant Monoclonal ATM antibody. Carrier free. Suitable for IP, WB and reacts with Human, Mouse, Rat samples. Cited in 1 publication.

View Alternative Names

Serine-protein kinase ATM, Ataxia telangiectasia mutated, A-T mutated, ATM

7 Images

Supplier Data

Immunoprecipitation - Anti-ATM antibody [EPR17059] - BSA and Azide free (AB232588)

ATM was immunoprecipitated from 1mg of HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate with ab199726 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab199726 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : HEK-293 whole cell lysate 10ug (Input). Lane 2 : ab199726 IP in HEK-293 whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab199726 in HEK-293 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 10 seconds.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199726).

All lanes:

Immunoprecipitation - Anti-ATM antibody [EPR17059] (<a href='/en-us/products/primary-antibodies/atm-antibody-epr17059-ab199726'>ab199726</a>)

Predicted band size: 351 kDa

false

Supplier Data

Immunoprecipitation - Anti-ATM antibody [EPR17059] - BSA and Azide free (AB232588)

ATM was immunoprecipitated from 1mg of SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate with ab199726 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab199726 at 1/500 dilution (Panel A) or ab32420 at 1/500 dilution (Panel B). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : SH-SY5Y whole cell lysate 10ug (Input). Lane 2 : ab199726 IP in SH-SY5Y whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab199726 in SH-SY5Y whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 30 seconds (Panel A and B).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199726).

All lanes:

Immunoprecipitation - Anti-ATM antibody [EPR17059] (<a href='/en-us/products/primary-antibodies/atm-antibody-epr17059-ab199726'>ab199726</a>)

Predicted band size: 351 kDa

false

Supplier Data

Immunoprecipitation - Anti-ATM antibody [EPR17059] - BSA and Azide free (AB232588)

ATM was immunoprecipitated from 1mg of HEK-293 (Human epithelial cells from embryonic kidney) whole cell lysate with ab199726 at 1/100 dilution. Western blot was performed from the immunoprecipitate using ab199726 at 1/500 dilution (Panel A) or ab32420 at 1/500 dilution (Panel B). Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.

Lane 1 : HEK-293 whole cell lysate 10ug (Input). Lane 2 : ab199726 IP in HEK-293 whole cell lysate. Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab199726 in HEK-293 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 30 seconds (Panel A and B).

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab199726).

All lanes:

Immunoprecipitation - Anti-ATM antibody [EPR17059] (<a href='/en-us/products/primary-antibodies/atm-antibody-epr17059-ab199726'>ab199726</a>)

Predicted band size: 351 kDa

false

Lab

Western blot - Anti-ATM antibody [EPR17059] - BSA and Azide free (AB232588)

False colour image of Western blot : Anti-ATM antibody [EPR17059] staining at 1/2000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab199726 was shown to bind specifically to ATM. A band was observed at 350 kDa in wild-type A549 cell lysates with no signal observed at this size in ATM knockout cell line ab276095 (knockout cell lysate ab283834). To generate this image, wild-type and ATM knockout A549 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 5 % milk in TBS-0.1 % Tween^® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preabsorbed (ab216776) at 1/20000 dilution.

All lanes:

Western blot - Anti-ATM antibody [EPR17059] (<a href='/en-us/products/primary-antibodies/atm-antibody-epr17059-ab199726'>ab199726</a>) at 1/2000 dilution

Lane 1:

Wild-type A549 cell lysate at 20 µg

Lane 2:

ATM knockout A549 cell lysate at 20 µg

Lane 2:

Western blot - Human ATM knockout A549 cell line (<a href='/en-us/products/cell-lines/human-atm-knockout-a549-cell-line-ab276095'>ab276095</a>)

Lane 3:

HEK-293 cell lysate at 20 µg

Lane 4:

U-2 OS cell lysate at 20 µg

Predicted band size: 351 kDa

Observed band size: 350 kDa

false

Supplier Data

Western blot - Anti-ATM antibody [EPR17059] - BSA and Azide free (AB232588)

This data was developed using ab199726, the same antibody clone in a different buffer formulation.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ATM antibody [EPR17059] - BSA and Azide free (ab232588) at 1/5000 dilution

All lanes:

293 (Human epithelial cells from embryonic kidney) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 351 kDa

Observed band size: 350 kDa

false

Exposure time: 3min

Supplier Data

Western blot - Anti-ATM antibody [EPR17059] - BSA and Azide free (AB232588)

This data was developed using ab199726, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-ATM antibody [EPR17059] - BSA and Azide free (ab232588) at 1/2000 dilution

Lane 1:

Rat testis lysate at 10 µg

Lane 2:

Mouse testis lysate at 10 µg

Lane 3:

293 (Human epithelial cells from embryonic kidney) whole cell lysate at 10 µg

Lane 4:

SH-SY5Y (Human neuroblastoma from bone marrow cells) whole cell lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 351 kDa

false

Exposure time: 20s

Supplier Data

Western blot - Anti-ATM antibody [EPR17059] - BSA and Azide free (AB232588)

This data was developed using ab199726, the same antibody clone in a different buffer formulation.

Blocking/Dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-ATM antibody [EPR17059] - BSA and Azide free (ab232588) at 1/2000 dilution

Lane 1:

Rat testis lysate at 10 µg

Lane 2:

Mouse testis lysate at 10 µg

Lane 3:

Mouse spleen lysate at 10 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1000 µg

Predicted band size: 351 kDa

Observed band size: 350 kDa

false

Exposure time: 3min

Unconjugated

Anti-ATM antibody [EPR17059]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17059

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

WB, IP

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "IP-species-checked": "guaranteed", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab232588 is the carrier-free version of ab199726.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATM also known as Ataxia Telangiectasia Mutated is a protein kinase with a molecular weight of approximately 370 kDa. ATM protein primarily resides in the cell nucleus and functions as a critical regulator of the cell cycle. It plays a significant role in the detection of DNA damage and initiation of repair processes. As part of its mechanical functions ATM phosphorylates serine and threonine residues on various substrates most notably in response to double-strand breaks in DNA. This activity is important for maintaining genomic stability.

Biological function summary

ATM acts as a coordinator in cellular response to DNA damage highly interacting with multiple components of the DNA repair machinery. It forms a complex with proteins like NBS1 and MRN complex facilitating repair by recruiting and activating other proteins involved in homologous recombination and non-homologous end joining pathways. ATM also modulates p53 activity a primary response factor in cellular stress management linking ATM to control of cell cycle arrest and apoptosis. This positions ATM as an integral part of maintaining cellular integrity in face of genomic insult.

Pathways

ATM integrates neatly within the DNA damage response and cell cycle control pathways. ATM's operative relationship with the MRN complex and its role in the PI3K-related protein kinase family helps initiate appropriate repair processes upon DNA damage detection. Additionally ATM regulates the activity of proteins such as Chk2 which further propagates signals to p53 influencing decisions between cell cycle arrest and apoptosis. These interactions link ATM closely to essential processes like DNA repair and cell survival highlighting its role in genomic maintenance.

ATM mutations or dysregulation leads to Ataxia Telangiectasia an autosomal recessive disorder characterized by neurodegeneration immune deficiencies and cancer predisposition. ATM dysfunction also connects to cancer development particularly breast cancer where it transmits signals involving BRCA1 contributing to DNA repair through homologous recombination. Understanding ATM dynamics and related pathways has important implications for developing therapeutic strategies to manage or mitigate effects associated with its dysfunction.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor (PubMed : 10550055, PubMed : 10839545, PubMed : 10910365, PubMed : 12556884, PubMed : 14871926, PubMed : 15064416, PubMed : 15448695, PubMed : 15456891, PubMed : 15790808, PubMed : 15916964, PubMed : 17923702, PubMed : 21757780, PubMed : 24534091, PubMed : 35076389, PubMed : 9733514). Recognizes the substrate consensus sequence [ST]-Q (PubMed : 10550055, PubMed : 10839545, PubMed : 10910365, PubMed : 12556884, PubMed : 14871926, PubMed : 15448695, PubMed : 15456891, PubMed : 15916964, PubMed : 17923702, PubMed : 24534091, PubMed : 9733514). Phosphorylates 'Ser-139' of histone variant H2AX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism (By similarity). Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates DYRK2, CHEK2, p53/TP53, FBXW7, FANCD2, NFKBIA, BRCA1, CREBBP/CBP, RBBP8/CTIP, FBXO46, MRE11, nibrin (NBN), RAD50, RAD17, PELI1, TERF1, UFL1, RAD9, UBQLN4 and DCLRE1C (PubMed : 10550055, PubMed : 10766245, PubMed : 10802669, PubMed : 10839545, PubMed : 10910365, PubMed : 10973490, PubMed : 11375976, PubMed : 12086603, PubMed : 15456891, PubMed : 19965871, PubMed : 21757780, PubMed : 24534091, PubMed : 26240375, PubMed : 26774286, PubMed : 30171069, PubMed : 30612738, PubMed : 30886146, PubMed : 30952868, PubMed : 38128537, PubMed : 9733515, PubMed : 9843217). May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends. Phosphorylation of DYRK2 in nucleus in response to genotoxic stress prevents its MDM2-mediated ubiquitination and subsequent proteasome degradation (PubMed : 19965871). Phosphorylates ATF2 which stimulates its function in DNA damage response (PubMed : 15916964). Phosphorylates ERCC6 which is essential for its chromatin remodeling activity at DNA double-strand breaks (PubMed : 29203878). Phosphorylates TTC5/STRAP at 'Ser-203' in the cytoplasm in response to DNA damage, which promotes TTC5/STRAP nuclear localization (PubMed : 15448695). Also involved in pexophagy by mediating phosphorylation of PEX5 : translocated to peroxisomes in response to reactive oxygen species (ROS), and catalyzes phosphorylation of PEX5, promoting PEX5 ubiquitination and induction of pexophagy (PubMed : 26344566).

See full target information ATM

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature 602:487-495 PubMed34942634

2021

Evolution of enhanced innate immune evasion by SARS-CoV-2.

Applications

Unspecified application

Species

Unspecified reactive species

Lucy G Thorne,Mehdi Bouhaddou,Ann-Kathrin Reuschl,Lorena Zuliani-Alvarez,Ben Polacco,Adrian Pelin,Jyoti Batra,Matthew V X Whelan,Myra Hosmillo,Andrea Fossati,Roberta Ragazzini,Irwin Jungreis,Manisha Ummadi,Ajda Rojc,Jane Turner,Marie L Bischof,Kirsten Obernier,Hannes Braberg,Margaret Soucheray,Alicia Richards,Kuei-Ho Chen,Bhavya Harjai,Danish Memon,Joseph Hiatt,Romel Rosales,Briana L McGovern,Aminu Jahun,Jacqueline M Fabius,Kris White,Ian G Goodfellow,Yasu Takeuchi,Paola Bonfanti,Kevan Shokat,Natalia Jura,Klim Verba,Mahdad Noursadeghi,Pedro Beltrao,Manolis Kellis,Danielle L Swaney,Adolfo García-Sastre,Clare Jolly,Greg J Towers,Nevan J Krogan

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATM antibody [EPR17059] - BSA and Azide free

Immunoprecipitation - Anti-ATM antibody [EPR17059] - BSA and Azide free (AB232588)

Immunoprecipitation - Anti-ATM antibody [EPR17059] - BSA and Azide free (AB232588)

Immunoprecipitation - Anti-ATM antibody [EPR17059] - BSA and Azide free (AB232588)

Western blot - Anti-ATM antibody [EPR17059] - BSA and Azide free (AB232588)

Western blot - Anti-ATM antibody [EPR17059] - BSA and Azide free (AB232588)

Western blot - Anti-ATM antibody [EPR17059] - BSA and Azide free (AB232588)

Western blot - Anti-ATM antibody [EPR17059] - BSA and Azide free (AB232588)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise