Skip to main content

Phospho ATM antibody pS1981 [EP1890Y] ab81292 is a rabbit monoclonal antibody that is used in ATM western blotting, IHC and flow cytometry. Suitable for human samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EP1890Y has been tried and trusted by researchers since 2009 and is cited in >400 publications
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation


Images

Immunoprecipitation - Anti-ATM (phospho S1981) antibody [EP1890Y] (AB81292), expandable thumbnail
  • Western blot - Anti-ATM (phospho S1981) antibody [EP1890Y] (AB81292), expandable thumbnail
  • Dot Blot - Anti-ATM (phospho S1981) antibody [EP1890Y] (AB81292), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] (AB81292), expandable thumbnail
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] (AB81292), expandable thumbnail

Publications

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPDotWBICC/IFFlow Cyt (Intra)IHC-P
Human
Tested
Expected
Tested
Not recommended
Tested
Tested
Synthetic peptide
Not recommended
Tested
Not recommended
Not recommended
Not recommended
Not recommended

Tested
Tested

Species

Human

Dilution info

1/30 - 1/40

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Synthetic peptide

Dilution info

1/100000

Notes

-

Expected
Expected

Species

Human

Dilution info

Use at an assay dependent concentration.

Notes

-

Tested
Tested

Species

Human

Dilution info

1/50000

Notes

We highly recommend using a positive control such as HEK293 cells treated with 10uM doxorubicin for 24 hours.

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/60

Notes

-

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

1/70 - 1/250

Notes

Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Synthetic peptide

Dilution info

-

Notes

-

Associated Products

Select an associated product type

15 products for Alternative Product

Target data

Function

Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates DYRK2, CHEK2, p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, UFL1, RAD9, UBQLN4 and DCLRE1C (PubMed:9843217, PubMed:9733515, PubMed:10550055, PubMed:10766245, PubMed:10839545, PubMed:10910365, PubMed:10802669, PubMed:10973490, PubMed:11375976, PubMed:12086603, PubMed:15456891, PubMed:19965871, PubMed:30612738, PubMed:30886146). May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends. Phosphorylation of DYRK2 in nucleus in response to genotoxic stress prevents its MDM2-mediated ubiquitination and subsequent proteasome degradation. Phosphorylates ATF2 which stimulates its function in DNA damage response. Phosphorylates ERCC6 which is essential for its chromatin remodeling activity at DNA double-strand breaks (PubMed:29203878).

Alternative names

Serine-protein kinase ATM, Ataxia telangiectasia mutated, A-T mutated, ATM

Recommended products

Phospho ATM antibody pS1981 [EP1890Y] ab81292 is a rabbit monoclonal antibody that is used in ATM western blotting, IHC and flow cytometry. Suitable for human samples.

- Recombinant format for unrivaled batch-batch consistency: no need for same-lot requests
- Antibody clone EP1890Y has been tried and trusted by researchers since 2009 and is cited in >400 publications
- Specificity and sensitivity confirmed in IHC with multi-tissue microarray (TMA) validation

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EP1890Y

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

ATM also known as Ataxia Telangiectasia Mutated is a protein kinase with a molecular weight of approximately 370 kDa. ATM protein primarily resides in the cell nucleus and functions as a critical regulator of the cell cycle. It plays a significant role in the detection of DNA damage and initiation of repair processes. As part of its mechanical functions ATM phosphorylates serine and threonine residues on various substrates most notably in response to double-strand breaks in DNA. This activity is important for maintaining genomic stability.

Biological function summary

ATM acts as a coordinator in cellular response to DNA damage highly interacting with multiple components of the DNA repair machinery. It forms a complex with proteins like NBS1 and MRN complex facilitating repair by recruiting and activating other proteins involved in homologous recombination and non-homologous end joining pathways. ATM also modulates p53 activity a primary response factor in cellular stress management linking ATM to control of cell cycle arrest and apoptosis. This positions ATM as an integral part of maintaining cellular integrity in face of genomic insult.

Pathways

ATM integrates neatly within the DNA damage response and cell cycle control pathways. ATM's operative relationship with the MRN complex and its role in the PI3K-related protein kinase family helps initiate appropriate repair processes upon DNA damage detection. Additionally ATM regulates the activity of proteins such as Chk2 which further propagates signals to p53 influencing decisions between cell cycle arrest and apoptosis. These interactions link ATM closely to essential processes like DNA repair and cell survival highlighting its role in genomic maintenance.

Associated diseases and disorders

ATM mutations or dysregulation leads to Ataxia Telangiectasia an autosomal recessive disorder characterized by neurodegeneration immune deficiencies and cancer predisposition. ATM dysfunction also connects to cancer development particularly breast cancer where it transmits signals involving BRCA1 contributing to DNA repair through homologous recombination. Understanding ATM dynamics and related pathways has important implications for developing therapeutic strategies to manage or mitigate effects associated with its dysfunction.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

10 product images

  • Immunoprecipitation - Anti-ATM (phospho S1981) antibody [EP1890Y] (ab81292), expandable thumbnail

    Immunoprecipitation - Anti-ATM (phospho S1981) antibody [EP1890Y] (ab81292)

    ATM was immunoprecipitated from HEK-293 (Human embryonic kidney epithelial cell) treated with Doxorubicin whole cell lysate with ab81292 at 1/30 dilution (5 μg in 1 mg lysates). Western blot was performed from the immunoprecipitate using ab81292 at 1/2000 dilution. An anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as secondary antibody at 1/1500 dilution.
    Lane 1: HEK-293 treated with Doxorubicin whole cell lysate 10 μg (Input).
    Lane 2: ab81292 IP in HEK-293 treated with Doxorubicin whole cell lysate.
    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab81292 in HEK-293 treated with Doxorubicin whole cell lysate.
    Blocking/Dilution buffer: 5% NFDM/TBST.
    Exposure time: 3 minutes.

    All lanes: Immunoprecipitation - Anti-ATM (phospho S1981) antibody [EP1890Y] (ab81292)

    Predicted band size: 351 kDa

  • Western blot - Anti-ATM (phospho S1981) antibody [EP1890Y] (ab81292), expandable thumbnail

    Western blot - Anti-ATM (phospho S1981) antibody [EP1890Y] (ab81292)

    Blocking and dilution buffer: 5% NFDM/TBST.

    All lanes: Western blot - Anti-ATM (phospho S1981) antibody [EP1890Y] (ab81292) at 1/50000 dilution

    Lane 1: HEK-293 (Human epithelial cell line from embryonic kidney) cell lysate, untreated at 10 µg

    Lane 2: HEK-293 cell lysate, treated with Doxorubicin at 10 µg

    Secondary

    All lanes: Peroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

    Predicted band size: 351 kDa

    Observed band size: 370 kDa

  • Dot Blot - Anti-ATM (phospho S1981) antibody [EP1890Y] (ab81292), expandable thumbnail

    Dot Blot - Anti-ATM (phospho S1981) antibody [EP1890Y] (ab81292)

    Dot blot analysis of ATM peptides using ab81292 at 1/000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100,000 dilution.

    Blocking and diluting buffer was 5% NFDM/TBST, exposure time 3 minutes.

    Lane 1: ATM (pS1981) phospho peptide

    Lane 2: ATM non-phospho peptide

    Lane 3: ATM (pS428) phospho peptide

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] (ab81292), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] (ab81292)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling ATM (phospho S1981) with purified ab81292 at 1/70. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, an HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500).

    Negative control using PBS instead of primary antibody.

    Counterstained with hematoxylin.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] (ab81292), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] (ab81292)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gastric carcinoma tissue labeling ATM with unpurified ab81292 at a 1/100 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] (ab81292), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] (ab81292)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labeling ATM with unpurified ab81292.


    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] (ab81292), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] (ab81292)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human tonsil tissue labeling ATM with unpurified ab81292.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] (ab81292), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] (ab81292)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labeling ATM with unpurified ab81292.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] (ab81292), expandable thumbnail

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] (ab81292)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial carcinoma tissue labeling ATM with unpurified ab81292.


    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Flow Cytometry (Intracellular) - Anti-ATM (phospho S1981) antibody [EP1890Y] (ab81292), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-ATM (phospho S1981) antibody [EP1890Y] (ab81292)

    Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma) cells labeling ATM (phospho S1981) with purified ab81292 at 1/60 dilution (10μg/mL) (red).

    Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluorr®488) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com