Rabbit Recombinant Monoclonal ATM phospho S1981 antibody. Carrier free. Suitable for Flow Cyt (Intra), IP, Dot, WB, IHC-P and reacts with Human, Synthetic peptide samples. Cited in 6 publications.
Images
![Immunoprecipitation - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (AB208775), expandable thumbnail](https://content.abcam.com/products/images/atm-phospho-s1981-antibody-ep1890y-bsa-and-azide-free-ab208775--immunoprecipitation-img4738.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (AB208775)")
![Western blot - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (AB208775), expandable thumbnail](https://content.abcam.com/products/images/atm-phospho-s1981-antibody-ep1890y-bsa-and-azide-free-ab208775--western-blot-img8539.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (AB208775)")
![Dot Blot - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (AB208775), expandable thumbnail](https://content.abcam.com/products/images/atm-phospho-s1981-antibody-ep1890y-bsa-and-azide-free-ab208775--dot-blot-img10896.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (AB208775)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (AB208775), expandable thumbnail](https://content.abcam.com/products/images/atm-phospho-s1981-antibody-ep1890y-bsa-and-azide-free-ab208775--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129915.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (AB208775)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (AB208775), expandable thumbnail](https://content.abcam.com/products/images/atm-phospho-s1981-antibody-ep1890y-bsa-and-azide-free-ab208775--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112729.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (AB208775)")
Publications
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- Aging 15:492-5122023Selective ATM inhibition augments radiation-induced inflammatory signaling and cancer cell death.Applications:Unspecified applicationReactive species:Unspecified reactive speciesLi-Ya Chiu,Qing Sun,Frank T Zenke,Andree Blaukat,Lyubomir T VassilevPubMed 36656721
- Autophagy 19:1479-14902022The telomeric protein TERF2/TRF2 impairs HMGB1-driven autophagy.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSara Iachettini,Fabio Ciccarone,Carmen Maresca,Carmen D' Angelo,Eleonora Petti,Serena Di Vito,Maria Rosa Ciriolo,Pasquale Zizza,Annamaria BiroccioPubMed 36310382
- Genetics in medicine : official journal of the American College of Medical Genetics 24:1821-18302022Cancer Risk C (CR-C), a functional genomics test is a sensitive and rapid test for germline mismatch repair deficiency.Applications:Unspecified applicationReactive species:Unspecified reactive speciesIshraq Alim,Johnny Loke,Sarah Yam,Allyson S Templeton,Polly Newcomb,Noralane M Lindor,Rish K Pai,Mark A Jenkins,Daniel D Buchanan,Steven Gallinger,Susan Klugman,Harry OstrerPubMed 35616648
- Molecular cancer therapeutics 21:859-8702022A New Class of Selective ATM Inhibitors as Combination Partners of DNA Double-Strand Break Inducing Cancer Therapies.Applications:Unspecified applicationReactive species:Unspecified reactive speciesAstrid Zimmermann,Frank T Zenke,Li-Ya Chiu,Heike Dahmen,Ulrich Pehl,Thomas Fuchss,Thomas Grombacher,Beatrix Blume,Lyubomir T Vassilev,Andree BlaukatPubMed 35405736
- Molecular oncology 16:2235-22592022ARID1A-deficient cells require HDAC6 for progression of endometrial carcinoma.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCristina Megino-Luque,Pol Sisó,Natalia Mota-Martorell,Raúl Navaridas,Inés de la Rosa,Izaskun Urdanibia,Manel Albertí-Valls,Maria Santacana,Miquel Pinyol,Núria Bonifaci,Anna Macià,David Llobet-Navas,Sònia Gatius,Xavier Matias-Guiu,Núria EritjaPubMed 35167193
- Scientific reports 11:121482021DNA-PK inhibitor peposertib enhances p53-dependent cytotoxicity of DNA double-strand break inducing therapy in acute leukemia.Applications:Unspecified applicationReactive species:Unspecified reactive speciesEric Haines,Yuki Nishida,Michael I Carr,Rafael Heinz Montoya,Lauren B Ostermann,Weiguo Zhang,Frank T Zenke,Andree Blaukat,Michael Andreeff,Lyubomir T VassilevPubMed 34108527
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt (Intra) | IP | Dot | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|---|---|
| Human | Tested | Tested | Expected | Tested | Not recommended | Tested |
| Synthetic peptide | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Please check the parent abID, Anti-ATM (phospho S1981) antibody [EP1890Y] ab81292, for more information on dilutions. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
15 products for Alternative Product
7 products for Alternative Version
Target data
Function
Serine/threonine protein kinase which activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates 'Ser-139' of histone variant H2AX at double strand breaks (DSBs), thereby regulating DNA damage response mechanism. Also plays a role in pre-B cell allelic exclusion, a process leading to expression of a single immunoglobulin heavy chain allele to enforce clonality and monospecific recognition by the B-cell antigen receptor (BCR) expressed on individual B-lymphocytes. After the introduction of DNA breaks by the RAG complex on one immunoglobulin allele, acts by mediating a repositioning of the second allele to pericentromeric heterochromatin, preventing accessibility to the RAG complex and recombination of the second allele. Also involved in signal transduction and cell cycle control. May function as a tumor suppressor. Necessary for activation of ABL1 and SAPK. Phosphorylates DYRK2, CHEK2, p53/TP53, FANCD2, NFKBIA, BRCA1, CTIP, nibrin (NBN), TERF1, UFL1, RAD9, UBQLN4 and DCLRE1C (PubMed:9843217, PubMed:9733515, PubMed:10550055, PubMed:10766245, PubMed:10839545, PubMed:10910365, PubMed:10802669, PubMed:10973490, PubMed:11375976, PubMed:12086603, PubMed:15456891, PubMed:19965871, PubMed:30612738, PubMed:30886146). May play a role in vesicle and/or protein transport. Could play a role in T-cell development, gonad and neurological function. Plays a role in replication-dependent histone mRNA degradation. Binds DNA ends. Phosphorylation of DYRK2 in nucleus in response to genotoxic stress prevents its MDM2-mediated ubiquitination and subsequent proteasome degradation. Phosphorylates ATF2 which stimulates its function in DNA damage response. Phosphorylates ERCC6 which is essential for its chromatin remodeling activity at DNA double-strand breaks (PubMed:29203878).
Alternative names
Serine-protein kinase ATM, Ataxia telangiectasia mutated, A-T mutated, ATM
Recommended products
Rabbit Recombinant Monoclonal ATM phospho S1981 antibody. Carrier free. Suitable for Flow Cyt (Intra), IP, Dot, WB, IHC-P and reacts with Human, Synthetic peptide samples. Cited in 6 publications.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
EP1890Y
- Purification technique
Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab208775 is the carrier-free version of Anti-ATM (phospho S1981) antibody [EP1890Y] ab81292.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
ATM also known as Ataxia Telangiectasia Mutated is a protein kinase with a molecular weight of approximately 370 kDa. ATM protein primarily resides in the cell nucleus and functions as a critical regulator of the cell cycle. It plays a significant role in the detection of DNA damage and initiation of repair processes. As part of its mechanical functions ATM phosphorylates serine and threonine residues on various substrates most notably in response to double-strand breaks in DNA. This activity is important for maintaining genomic stability.
- Biological function summary
ATM acts as a coordinator in cellular response to DNA damage highly interacting with multiple components of the DNA repair machinery. It forms a complex with proteins like NBS1 and MRN complex facilitating repair by recruiting and activating other proteins involved in homologous recombination and non-homologous end joining pathways. ATM also modulates p53 activity a primary response factor in cellular stress management linking ATM to control of cell cycle arrest and apoptosis. This positions ATM as an integral part of maintaining cellular integrity in face of genomic insult.
- Pathways
ATM integrates neatly within the DNA damage response and cell cycle control pathways. ATM's operative relationship with the MRN complex and its role in the PI3K-related protein kinase family helps initiate appropriate repair processes upon DNA damage detection. Additionally ATM regulates the activity of proteins such as Chk2 which further propagates signals to p53 influencing decisions between cell cycle arrest and apoptosis. These interactions link ATM closely to essential processes like DNA repair and cell survival highlighting its role in genomic maintenance.
- Associated diseases and disorders
ATM mutations or dysregulation leads to Ataxia Telangiectasia an autosomal recessive disorder characterized by neurodegeneration immune deficiencies and cancer predisposition. ATM dysfunction also connects to cancer development particularly breast cancer where it transmits signals involving BRCA1 contributing to DNA repair through homologous recombination. Understanding ATM dynamics and related pathways has important implications for developing therapeutic strategies to manage or mitigate effects associated with its dysfunction.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
10 product images
![Immunoprecipitation - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775), expandable thumbnail](https://content.abcam.com/products/images/atm-phospho-s1981-antibody-ep1890y-bsa-and-azide-free-ab208775--immunoprecipitation-img4738.jpg "Immunoprecipitation - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)")
Immunoprecipitation - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)
ATM was immunoprecipitated from HEK-293 (Human embryonic kidney epithelial cell) treated with Doxorubicin whole cell lysate with Anti-ATM (phospho S1981) antibody [EP1890Y] ab81292 at 1/30 dilution (5 µg in 1 mg lysates). Western blot was performed from the immunoprecipitate using Anti-ATM (phospho S1981) antibody [EP1890Y] ab81292 at 1/2000 dilution. An anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as secondary antibody at 1/1500 dilution.
Lane 1: HEK-293 treated with Doxorubicin whole cell lysate 10 µg (Input).
Lane 2: Anti-ATM (phospho S1981) antibody [EP1890Y] ab81292 IP in HEK-293 treated with Doxorubicin whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-ATM (phospho S1981) antibody [EP1890Y] ab81292 in HEK-293 treated with Doxorubicin whole cell lysate.
Blocking/Dilution buffer: 5% NFDM/TBST.
Exposure time: 3 minutes.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ATM (phospho S1981) antibody [EP1890Y] ab81292).
All lanes: Immunoprecipitation - Anti-ATM (phospho S1981) antibody [EP1890Y] (Anti-ATM (phospho S1981) antibody [EP1890Y] ab81292)
Predicted band size: 351 kDa
![Western blot - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775), expandable thumbnail](https://content.abcam.com/products/images/atm-phospho-s1981-antibody-ep1890y-bsa-and-azide-free-ab208775--western-blot-img8539.jpg "Western blot - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)")
Western blot - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)
Blocking buffer and concentration: 5% NFDM/TBST
Diluting buffer and concentration: 5% NFDM/TBST
All lanes: Western blot - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)
All lanes: HEK293 (human embryonic kidney) treated with Doxorubicin whole cell lysate at 10 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051)
Predicted band size: 351 kDa
Observed band size: 370 kDa
Exposure time: 1min
![Dot Blot - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775), expandable thumbnail](https://content.abcam.com/products/images/atm-phospho-s1981-antibody-ep1890y-bsa-and-azide-free-ab208775--dot-blot-img10896.jpg "Dot Blot - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)")
Dot Blot - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)
Dot blot analysis of ATM peptides using Anti-ATM (phospho S1981) antibody [EP1890Y] ab81292 at 1/000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100,000 dilution.
Blocking and diluting buffer was 5% NFDM/TBST, exposure time 3 minutes.
Lane 1: ATM (pS1981) phospho peptide
Lane 2: ATM non-phospho peptide
Lane 3: ATM (pS428) phospho peptide
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ATM (phospho S1981) antibody [EP1890Y] ab81292).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775), expandable thumbnail](https://content.abcam.com/products/images/atm-phospho-s1981-antibody-ep1890y-bsa-and-azide-free-ab208775--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img129915.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling ATM (phospho S1981) with purified Anti-ATM (phospho S1981) antibody [EP1890Y] ab81292 at 1/70. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. Goat Anti-Rabbit IgG H&L (HRP) ab97051, an HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500).
Negative control using PBS instead of primary antibody.
Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ATM (phospho S1981) antibody [EP1890Y] ab81292).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775), expandable thumbnail](https://content.abcam.com/products/images/atm-phospho-s1981-antibody-ep1890y-bsa-and-azide-free-ab208775--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img112729.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gastric carcinoma tissue labeling ATM with unpurified Anti-ATM (phospho S1981) antibody [EP1890Y] ab81292 at a 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ATM (phospho S1981) antibody [EP1890Y] ab81292).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775), expandable thumbnail](https://content.abcam.com/products/images/atm-phospho-s1981-antibody-ep1890y-bsa-and-azide-free-ab208775--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img54679.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labeling ATM with unpurified Anti-ATM (phospho S1981) antibody [EP1890Y] ab81292.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ATM (phospho S1981) antibody [EP1890Y] ab81292).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775), expandable thumbnail](https://content.abcam.com/products/images/atm-phospho-s1981-antibody-ep1890y-bsa-and-azide-free-ab208775--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img3094.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human tonsil tissue labeling ATM with unpurified Anti-ATM (phospho S1981) antibody [EP1890Y] ab81292.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ATM (phospho S1981) antibody [EP1890Y] ab81292).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775), expandable thumbnail](https://content.abcam.com/products/images/atm-phospho-s1981-antibody-ep1890y-bsa-and-azide-free-ab208775--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img89867.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labeling ATM with unpurified Anti-ATM (phospho S1981) antibody [EP1890Y] ab81292.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ATM (phospho S1981) antibody [EP1890Y] ab81292).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775), expandable thumbnail](https://content.abcam.com/products/images/atm-phospho-s1981-antibody-ep1890y-bsa-and-azide-free-ab208775--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126268.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial carcinoma tissue labeling ATM with unpurified Anti-ATM (phospho S1981) antibody [EP1890Y] ab81292.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ATM (phospho S1981) antibody [EP1890Y] ab81292).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
![Flow Cytometry (Intracellular) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775), expandable thumbnail](https://content.abcam.com/products/images/atm-phospho-s1981-antibody-ep1890y-bsa-and-azide-free-ab208775--flow-cytometry-intracellular-img414873.jpg "Flow Cytometry (Intracellular) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)")
Flow Cytometry (Intracellular) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)
Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma) cells labeling ATM (phospho S1981) with purified Anti-ATM (phospho S1981) antibody [EP1890Y] ab81292 at 1/60 dilution (10 μg/mL) (red).
Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor®488) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-ATM (phospho S1981) antibody [EP1890Y] ab81292).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775), expandable thumbnail](https://content.abcam.com/products/images/atm-phospho-s1981-antibody-ep1890y-bsa-and-azide-free-ab208775--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img54679.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775), expandable thumbnail](https://content.abcam.com/products/images/atm-phospho-s1981-antibody-ep1890y-bsa-and-azide-free-ab208775--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img3094.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775), expandable thumbnail](https://content.abcam.com/products/images/atm-phospho-s1981-antibody-ep1890y-bsa-and-azide-free-ab208775--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img89867.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775), expandable thumbnail](https://content.abcam.com/products/images/atm-phospho-s1981-antibody-ep1890y-bsa-and-azide-free-ab208775--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img126268.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)")
![Flow Cytometry (Intracellular) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775), expandable thumbnail](https://content.abcam.com/products/images/atm-phospho-s1981-antibody-ep1890y-bsa-and-azide-free-ab208775--flow-cytometry-intracellular-img414873.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)")
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