Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free
- RabMAb
- Recombinant
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(48 Publications)
Rabbit Recombinant Monoclonal ATM phospho S1981 antibody. Carrier free. Suitable for Flow Cyt (Intra), IP, Dot, WB, IHC-P and reacts with Human, Synthetic peptide samples. Cited in 48 publications.
View Alternative Names
Serine-protein kinase ATM, Ataxia telangiectasia mutated, A-T mutated, ATM
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (AB208775)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of normal human tonsil tissue labeling ATM with unpurified ab81292.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81292).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (AB208775)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human cervical carcinoma tissue labeling ATM with unpurified ab81292.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81292).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (AB208775)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labeling ATM with unpurified ab81292.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81292).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (AB208775)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human hepatocellular carcinoma tissue labelling ATM (phospho S1981) with purified ab81292 at 1/70. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, an HRP-conjugated goat anti-rabbit IgG (H+L) was used as the secondary antibody (1/500).
Negative control using PBS instead of primary antibody.
Counterstained with hematoxylin.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81292).
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (AB208775)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial carcinoma tissue labeling ATM with unpurified ab81292.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81292).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- Flow Cyt (Intra)
Unknown
Flow Cytometry (Intracellular) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (AB208775)
Intracellular Flow Cytometry analysis of HepG2 (Human hepatocellular carcinoma) cells labeling ATM (phospho S1981) with purified ab81292 at 1/60 dilution (10 μg/mL) (red).
Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor®488) at 1/2000 dilution was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81292).
- IHC-P
Unknown
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (AB208775)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human gastric carcinoma tissue labeling ATM with unpurified ab81292 at a 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81292).
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
- IP
Unknown
Immunoprecipitation - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (AB208775)
ATM was immunoprecipitated from HEK-293 (Human embryonic kidney epithelial cell) treated with Doxorubicin whole cell lysate with ab81292 at 1/30 dilution (5 µg in 1 mg lysates). Western blot was performed from the immunoprecipitate using ab81292 at 1/2000 dilution. An anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG was used as secondary antibody at 1/1500 dilution.
Lane 1 : HEK-293 treated with Doxorubicin whole cell lysate 10 µg (Input).
Lane 2 : ab81292 IP in HEK-293 treated with Doxorubicin whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab81292 in HEK-293 treated with Doxorubicin whole cell lysate.
Blocking/Dilution buffer : 5% NFDM/TBST.
Exposure time : 3 minutes.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81292).
All lanes:
Immunoprecipitation - Anti-ATM (phospho S1981) antibody [EP1890Y] (<a href='/en-us/products/primary-antibodies/atm-phospho-s1981-antibody-ep1890y-ab81292'>ab81292</a>)
Predicted band size: 351 kDa
false
- WB
Lab
Western blot - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (AB208775)
Blocking buffer and concentration : 5% NFDM/TBST
Diluting buffer and concentration : 5% NFDM/TBST
All lanes:
Western blot - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (ab208775)
All lanes:
HEK293 (human embryonic kidney) treated with Doxorubicin whole cell lysate at 10 µg
Secondary
All lanes:
Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>)
Predicted band size: 351 kDa
Observed band size: 370 kDa
false
Exposure time: 1min
- Dot
Unknown
Dot Blot - Anti-ATM (phospho S1981) antibody [EP1890Y] - BSA and Azide free (AB208775)
Dot blot analysis of ATM peptides using ab81292 at 1/000 dilution followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody (ab97051) at 1/100,000 dilution.
Blocking and diluting buffer was 5% NFDM/TBST, exposure time 3 minutes.
Lane 1 : ATM (pS1981) phospho peptide
Lane 2 : ATM non-phospho peptide
Lane 3 : ATM (pS428) phospho peptide
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab81292).
Related conjugates and formulations (10)
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Anti-ATM (phospho S1981) antibody [EP1890Y]
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775 Alexa Fluor® 750
Alexa Fluor® 750 Anti-ATM (phospho S1981) antibody [EP1890Y]
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578 PE
PE Anti-ATM (phospho S1981) antibody [EP1890Y]
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660 APC
APC Anti-ATM (phospho S1981) antibody [EP1890Y]
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HRP Anti-ATM (phospho S1981) antibody [EP1890Y]
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519 Alexa Fluor® 488
Alexa Fluor® 488 Anti-ATM (phospho S1981) antibody [EP1890Y]
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665 Alexa Fluor® 647
Alexa Fluor® 647 Anti-ATM (phospho S1981) antibody [EP1890Y]
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617 Alexa Fluor® 594
Alexa Fluor® 594 Anti-ATM (phospho S1981) antibody [EP1890Y]
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565 Alexa Fluor® 555
Alexa Fluor® 555 Anti-ATM (phospho S1981) antibody [EP1890Y]
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603 Alexa Fluor® 568
Alexa Fluor® 568 Anti-ATM (phospho S1981) antibody [EP1890Y]
Reactivity data
Product details
ab208775 is the carrier-free version of ab81292.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Species reactivity
Rat: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Properties and storage information
Form
Purification technique
Storage buffer
Shipped at conditions
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
ATM acts as a coordinator in cellular response to DNA damage highly interacting with multiple components of the DNA repair machinery. It forms a complex with proteins like NBS1 and MRN complex facilitating repair by recruiting and activating other proteins involved in homologous recombination and non-homologous end joining pathways. ATM also modulates p53 activity a primary response factor in cellular stress management linking ATM to control of cell cycle arrest and apoptosis. This positions ATM as an integral part of maintaining cellular integrity in face of genomic insult.
Pathways
ATM integrates neatly within the DNA damage response and cell cycle control pathways. ATM's operative relationship with the MRN complex and its role in the PI3K-related protein kinase family helps initiate appropriate repair processes upon DNA damage detection. Additionally ATM regulates the activity of proteins such as Chk2 which further propagates signals to p53 influencing decisions between cell cycle arrest and apoptosis. These interactions link ATM closely to essential processes like DNA repair and cell survival highlighting its role in genomic maintenance.
Product protocols
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Target data
Publications (48)
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Aging 15:492-512 PubMed36656721
2023
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Autophagy 19:1479-1490 PubMed36310382
2022
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Genetics in medicine : official journal of the American College of Medical Genetics 24:1821-1830 PubMed35616648
2022
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Molecular cancer therapeutics 21:859-870 PubMed35405736
2022
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Molecular oncology 16:2235-2259 PubMed35167193
2022
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Scientific reports 11:12148 PubMed34108527
2021
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Nature communications 5:5691 PubMed25487737
2014
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PloS one 9:e113424 PubMed25409520
2014
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Oncogene 34:3977-84 PubMed25308476
2014
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PloS one 9:e104732 PubMed25111195
2014
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Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com