Anti-ATM (phospho S1987) antibody [EPR28058-71]

6 product images

• 

  Western blot - Anti-ATM (phospho S1987) antibody [EPR28058-71] (ab315019)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  The identity of the lower MW band at approximately 190 kDa and higher MW band at approximately 460 kDa are unknown.

  In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

  In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (Anti-6X His tag® antibody [EPR20547] - ChIP Grade ab213204) staining at 1/5000 dilution.

  All lanes: Western blot - Anti-ATM (phospho S1987) antibody [EPR28058-71] (ab315019) at 1/1000 dilution

  Lane 1: 293T cells transfected with an empty vector containing a myc-His-tag®, whole cell lysate at 20 µg

  Lane 2: 293T cells transfected with a mouse wild-type ATM expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

  Lane 3: 293T cells transfected with a mouse ATM (S1987A mutation) expression vector containing a myc-His-tag®, whole cell lysate at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 349 kDa, 124 kDa

  Exposure time: 180s

• 

  Immunocytochemistry/ Immunofluorescence - Anti-ATM (phospho S1987) antibody [EPR28058-71] (ab315019)

  Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized MEF (mouse embryo fibroblast) cells labelling ATM (phospho S1987) with ab315019 at 1/100 (5.11 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green). Confocal image showing increased nuclear staining in MEF cells treated with UV-C 100 J/m2, then recovery for 4 hours. Image was taken with a confocal microscope(Leica-Microsystems, TCS SP8). Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).

  Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• 

  Western blot - Anti-ATM (phospho S1987) antibody [EPR28058-71] (ab315019)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  The identity of the lower MW band at approximately 190 kDa is unknown.

  In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

  In Western blot, Anti-ATM antibody [EPR20100] - (Anti-ATM antibody [EPR20100] - ChIP Grade ab201022) staining at 1/1000 dilution.

  All lanes: Western blot - Anti-ATM (phospho S1987) antibody [EPR28058-71] (ab315019) at 1/1000 dilution

  Lane 1: Untreated NIH/3T3 (mouse embryonic fibroblast) whole cell lysate (untreated membrane) at 20 µg

  Lane 2: NIH/3T3 treated with UV-C for 100J/cm2, then recovery 2 hours, whole cell lysate (untreated membrane) at 20 µg

  Lane 3: NIH/3T3 treated with UV-C for 100J/cm2, then recovery 2 hours, whole cell lysate (phosphatase treated membrane) at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 349 kDa, 124 kDa

  Exposure time: 81s

• 

  Western blot - Anti-ATM (phospho S1987) antibody [EPR28058-71] (ab315019)

  Blocking and diluting buffer and concentration: 5% NFDM/TBST.

  The identity of the lower MW band at approximately 190 kDa is unknown.

  In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] ab129002) staining at 1/10000 dilution.

  In Western blot, Anti-ATM antibody [EPR20100] - (Anti-ATM antibody [EPR20100] - ChIP Grade ab201022) staining at 1/1000 dilution.

  All lanes: Western blot - Anti-ATM (phospho S1987) antibody [EPR28058-71] (ab315019) at 1/1000 dilution

  Lane 1: Untreated MEF (mouse embryo fibroblast) whole cell lysate (untreated membrane) at 20 µg

  Lane 2: MEF treated with UV-C for 100J/m2, then recovery 4 hours, whole cell lysate (untreated membrane) at 20 µg

  Lane 3: MEF treated with UV-C for 100J/m2, then recovery 4 hours, whole cell lysate (phosphatase treated membrane) at 20 µg

  Secondary

  All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution

  Observed band size: 349 kDa, 124 kDa

  Exposure time: 81s

• 

  Dot Blot - Anti-ATM (phospho S1987) antibody [EPR28058-71] (ab315019)

  Dot blot analysis of ATM (phospho S1987) using ab315019 at 1:1000 (0.511 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.

  Exposure time: 180 seconds

  Blocking and diluting buffer and concentration: 5% NFDM/TBST

• 

  Flow Cytometry (Intracellular) - Anti-ATM (phospho S1987) antibody [EPR28058-71] (ab315019)

  Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized MEF (mouse embryo fibroblast) treated with 100 J/m2 UV-C then recovery for 4h (Red) / Untreated MEF (Green) cells labelling ATM (phospho S1987) with ab315019 at 1/500 dilution (0.1 ug)/Red and Green compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.