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Rabbit Recombinant Monoclonal ATOX1 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples.

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Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.
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Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Human
Tested
Not recommended
Tested
Expected
Not recommended

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Tested
Tested

Species

Human

Dilution info

-

Notes

-

Expected
Expected

Species

Human

Dilution info

-

Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species

Human

Dilution info

-

Notes

-

Target data

Function

Binds and deliver cytosolic copper to the copper ATPase proteins. May be important in cellular antioxidant defense.

Alternative names

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Rabbit Recombinant Monoclonal ATOX1 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples.

Alternative names

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free

Yes

Clone number

EPR10352

Purification technique

Affinity purification

Concentration
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Storage

Shipped at conditions

Blue Ice

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

Notes

ab249079 is the carrier-free version of ab154179.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

Biological function summary

ATOX1 serves as a chaperone that transports copper ions to ATPase proteins like ATP7A and ATP7B in the secretory pathway. ATOX1 works individually rather than as part of a larger protein complex handling copper metabolism effectively. By facilitating the delivery of copper to essential enzymes ATOX1 assists in maintaining critical cellular functions such as oxidative stress response and energy production.

Activity summary

ATOX1 also known as Antioxidant 1 Copper Chaperone is a small copper-binding protein with a molecular mass of approximately 7 kDa. It plays an essential mechanical role in the transport of copper ions within the cell. ATOX1 helps deliver copper to key enzymes involved in cellular processes. It is expressed in various tissue types including the liver and the brain facilitating copper homeostasis at a cellular level.

Pathways

Copper ion transport involving ATOX1 plays an important role in the regulation of metal ion homeostasis pathways. ATOX1 interacts closely with ATP7A and ATP7B transporting copper ions necessary for redox reactions and enzyme activities in these pathways. ATOX1's role in copper regulation also aligns with antioxidant defense processes helping protect cells from damage due to reactive oxygen species.

Associated diseases and disorders

Disruption in ATOX1 function can be linked to disorders such as Wilson's disease and Menkes disease both of which involve copper metabolism impairment. Wilson's disease involves the protein ATP7B where the absence of proper copper transport leads to toxicity. Menkes disease involves ATP7A mutations leading to a shortage of copper delivery to necessary enzymes. ATOX1's activity is important for delivering copper to these associated ATPases highlighting its importance in related pathologies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-ATOX1 antibody [EPR10352] - BSA and Azide free (ab249079), expandable thumbnail

    Western blot - Anti-ATOX1 antibody [EPR10352] - BSA and Azide free (ab249079)

    This data was developed using ab154179, the same antibody clone in a different buffer formulation.

    Lanes 1-4: Merged signal (red and green). Green - ab154179 observed at 7 kDa. Red - loading control ab8245 observed at 36 kDa.

    ab154179 Anti-ATOX1 antibody [EPR10352] was shown to specifically react with ATOX1 in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266651 (knockout cell lysate ab257849) was used. Wild-type and ATOX1 knockout samples were subjected to SDS-PAGE. ab154179 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 500 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-ATOX1 antibody [EPR10352] (AB154179) at 1/500 dilution

    Lane 1: Wild-type HEK293T cell lysate at 20 µg

    Lane 2: ATOX1 knockout HEK293T cell lysate at 20 µg

    Lane 3: HeLa cell lysate at 20 µg

    Secondary

    All lanes: Western blot - Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (AB216773) at 1/10000 dilution

    Predicted band size: 36 kDa, 45 kDa, 62 kDa, 7 kDa

    Observed band size: 48 kDa, 50 kDa, 7 kDa, 70 kDa

  • Western blot - Anti-ATOX1 antibody [EPR10352] - BSA and Azide free (ab249079), expandable thumbnail

    Western blot - Anti-ATOX1 antibody [EPR10352] - BSA and Azide free (ab249079)

    This data was developed using the same antibody clone in a different buffer formulation (ab154179).

    Lanes 1 - 2: Merged signal (red and green). Green - ab154179 observed at 7 kDa. Red - loading control ab7291 (Mouse anti-Alpha Tubulin [DM1A] observed at 55kDa.

    ab154179 was shown to react with ATOX1 in HEK-293 wild-type cells in western blot with loss of signal observed in ATOX1 knockout sample. HEK-293 wild-type and ATOX1 knockout cell lysates were subjected to SDS-PAGE. Membranes were blocked in 3% milk in TBS-T (0.1% Tween®) before incubation with ab154179 and ab7291 (Mouse anti-Alpha Tubulin [DM1A] overnight at 4°C at a 1 in 1000 Dilution and a 1 in 20000 dilution respectively. Blots were incubated with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

    All lanes: Western blot - Anti-ATOX1 antibody [EPR10352] (AB154179) at 1/1000 dilution

    Lane 1: Wild-type HEK-293 cell lysate at 20 µg

    Lane 2: ATOX1 knockout HEK-293T cell lysate at 20 µg

    Performed under reducing conditions.

    Predicted band size: 7 kDa

    Observed band size: 7 kDa

  • Immunoprecipitation - Anti-ATOX1 antibody [EPR10352] - BSA and Azide free (ab249079), expandable thumbnail

    Immunoprecipitation - Anti-ATOX1 antibody [EPR10352] - BSA and Azide free (ab249079)

    This data was developed using ab154179, the same antibody clone in a different buffer formulation.ATOX1 was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg with ab154179 at 1/20 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg

    Lane 2: abab154179 IP in HeLa whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab154179 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-ATOX1 antibody [EPR10352] (AB154179)

    Predicted band size: 7 kDa

    Observed band size: 7 kDa

  • Western blot - Anti-ATOX1 antibody [EPR10352] - BSA and Azide free (ab249079), expandable thumbnail

    Western blot - Anti-ATOX1 antibody [EPR10352] - BSA and Azide free (ab249079)

    This data was developed using ab154179, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-ATOX1 antibody [EPR10352] (AB154179) at 1/1000 dilution

    Lane 1: HepG2 cell lysates at 10 µg

    Lane 2: HeLa cell lysates at 10 µg

    Lane 3: 293T cell lysates at 10 µg

    Secondary

    All lanes: HRP labelled goat anti-rabbit at 1/2000 dilution

    Predicted band size: 7 kDa

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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