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Rabbit Recombinant Monoclonal ATOX1 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples.

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Images

Western blot - Anti-ATOX1 antibody [EPR10352] - BSA and Azide free (AB249079), expandable thumbnail
  • Western blot - Anti-ATOX1 antibody [EPR10352] - BSA and Azide free (AB249079), expandable thumbnail
  • Immunoprecipitation - Anti-ATOX1 antibody [EPR10352] - BSA and Azide free (AB249079), expandable thumbnail
  • Western blot - Anti-ATOX1 antibody [EPR10352] - BSA and Azide free (AB249079), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPFlow CytWBIHC-PICC/IF
Human
Tested
Not recommended
Tested
Expected
Not recommended

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Expected
Expected

Species
Human
Dilution info
-
Notes

Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Target data

Function

Binds and deliver cytosolic copper to the copper ATPase proteins. May be important in cellular antioxidant defense.

Alternative names

HAH1, ATOX1, Copper transport protein ATOX1, Metal transport protein ATX1

Recommended products

Rabbit Recombinant Monoclonal ATOX1 antibody. Carrier free. Suitable for IP, WB, IHC-P and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR10352
Purification technique
Affinity purification
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab249079 is the carrier-free version of Anti-ATOX1 antibody [EPR10352] ab154179.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

ATOX1 also known as Antioxidant 1 Copper Chaperone is a small copper-binding protein with a molecular mass of approximately 7 kDa. It plays an essential mechanical role in the transport of copper ions within the cell. ATOX1 helps deliver copper to key enzymes involved in cellular processes. It is expressed in various tissue types including the liver and the brain facilitating copper homeostasis at a cellular level.

Biological function summary

ATOX1 serves as a chaperone that transports copper ions to ATPase proteins like ATP7A and ATP7B in the secretory pathway. ATOX1 works individually rather than as part of a larger protein complex handling copper metabolism effectively. By facilitating the delivery of copper to essential enzymes ATOX1 assists in maintaining critical cellular functions such as oxidative stress response and energy production.

Pathways

Copper ion transport involving ATOX1 plays an important role in the regulation of metal ion homeostasis pathways. ATOX1 interacts closely with ATP7A and ATP7B transporting copper ions necessary for redox reactions and enzyme activities in these pathways. ATOX1's role in copper regulation also aligns with antioxidant defense processes helping protect cells from damage due to reactive oxygen species.

Associated diseases and disorders

Disruption in ATOX1 function can be linked to disorders such as Wilson's disease and Menkes disease both of which involve copper metabolism impairment. Wilson's disease involves the protein ATP7B where the absence of proper copper transport leads to toxicity. Menkes disease involves ATP7A mutations leading to a shortage of copper delivery to necessary enzymes. ATOX1's activity is important for delivering copper to these associated ATPases highlighting its importance in related pathologies.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com