AB99856

Anti-ATP-binding cassette sub-family A member 3 antibody

Name: Anti-ATP-binding cassette sub-family A member 3 antibody (ab99856) | Abcam
Brand: Abcam Limited
SKU: AB99856
Price: 435 USD
Availability: InStock
Rating: 5 (2 reviews)

(2 Reviews)

(6 Publications)

Rabbit Polyclonal ATP-binding cassette sub-family A member 3 antibody. Suitable for WB, ICC/IF and reacts with Human samples. Cited in 6 publications. Immunogen corresponding to Recombinant Fragment Protein within Human ABCA3 aa 1 to C-terminus.

View Alternative Names

ABC3, ABCA3, Phospholipid-transporting ATPase ABCA3, ABC-C transporter, ATP-binding cassette sub-family A member 3, ATP-binding cassette transporter 3, Xenobiotic-transporting ATPase ABCA3, ATP-binding cassette 3

1 Images

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Western blot - Anti-ATP-binding cassette sub-family A member 3 antibody (AB99856)

All lanes:

Western blot - Anti-ATP-binding cassette sub-family A member 3 antibody (ab99856) at 1/1000 dilution

Lane 1:

SW480 cell lysate

Lane 2:

Hela cell lysate

Lane 3:

Human fetal kidney lysate

Lane 4:

Human fetal lung lysate

Predicted band size: 191 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human ABCA3 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q99758

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP-binding cassette sub-family A member 3 (ABCA3) also known as Surfactant Metabolism Dysfunction Pulmonary 3 is a vital membrane protein weighing approximately 170 kDa. It belongs to the ATP-binding cassette (ABC) protein family which also includes family binding proteins that transport various molecules across cellular membranes. ABCA3 is mainly expressed in alveolar type II cells of the lung where it plays a significant mechanical role in surfactant metabolism. The protein facilitates the transport of lipids into lamellar bodies which are critical for surfactant storage and secretion.

Biological function summary

ABCA3 is essential for normal pulmonary function as it contributes to surfactant phospholipid homeostasis. It operates as a monomer and is part of a larger complex with other lipid-processing proteins. This interaction ensures the correct assembly and secretion of pulmonary surfactant which reduces surface tension in the alveoli and prevents alveolar collapse. The dysfunction or absence of ABCA3 can disrupt these processes compromising lung function.

Pathways

The role of ABCA3 is significant in the surfactant metabolism pathway and lipid transportation processes. The surfactant metabolism pathway ensures the availability of pulmonary surfactant necessary for efficient respiratory function. In these pathways ABCA3 intersects with other proteins such as ABCA1 and proteins involved in the phospholipid synthesis and transport processes ensuring lipid proteins are correctly assembled for surfactant formation.

ABCA3 mutations associate with respiratory diseases such as Neonatal Respiratory Distress Syndrome and interstitial lung disease. These diseases often result from impaired surfactant production and secretion caused by ABCA3 deficiency. Mutations in this protein can also lead to dysfunctional interactions with partner proteins like Surfactant Protein B (SP-B) and Surfactant Protein C (SP-C) further implicating its role in disease pathology. Understanding the connection between ABCA3 and these proteins reveals potential therapeutic targets for treating associated pulmonary conditions.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Catalyzes the ATP-dependent transport of phospholipids such as phosphatidylcholine and phosphoglycerol from the cytoplasm into the lumen side of lamellar bodies, in turn participates in the lamellar bodies biogenesis and homeostasis of pulmonary surfactant (PubMed : 16959783, PubMed : 17574245, PubMed : 27177387, PubMed : 28887056, PubMed : 31473345). Transports preferentially phosphatidylcholine containing short acyl chains (PubMed : 27177387). In addition plays a role as an efflux transporter of miltefosine across macrophage membranes and free cholesterol (FC) through intralumenal vesicles by removing FC from the cell as a component of surfactant and protects cells from free cholesterol toxicity (PubMed : 25817392, PubMed : 26903515, PubMed : 27177387).

See full target information ABCA3

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Publications (6)

Recent publications for all applications. Explore the full list and refine your search

Journal of nanobiotechnology 23:339 PubMed40340852

2025

Dual-responsive nanoparticles targeting ACE-II senescence for therapeutic mitigation of acute lung injury.

Applications

Unspecified application

Species

Unspecified reactive species

Linlin Gao,Fushuang Zheng,Zhiling Fu,Wei Wang

Journal of neurology 271:2736-2744 PubMed38386048

2024

Different pain phenotypes are associated with anti-Caspr2 autoantibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Patrik Greguletz,Maria Plötz,Carolin Baade-Büttner,Christian G Bien,Katharina Eisenhut,Christian Geis,Robert Handreka,Jaqueline Klausewitz,Peter Körtvelyessy,Stjepana Kovac,Andrea Kraft,Jan Lewerenz,Michael Malter,Michael Nagel,Felix von Podewils,Harald Prüß,Anna Rada,Johanna Rau,Sebastian Rauer,Rosa Rößling,Thomas Seifert-Held,Kai Siebenbrodt,Kurt-Wolfram Sühs,Simone C Tauber,Franziska Thaler,Judith Wagner,Jonathan Wickel,Frank Leypoldt,Heike L Rittner,Claudia Sommer,Carmen Villmann,Kathrin Doppler

Clinical epigenetics 15:182 PubMed37951958

2023

Histone deacetylase 3 deletion in alveolar type 2 epithelial cells prevents bleomycin-induced pulmonary fibrosis.

Applications

Unspecified application

Species

Unspecified reactive species

Rui Xiong,Boxin Geng,Wenyang Jiang,Yong Hu,Zhaoyu Hu,Bo Hao,Ning Li,Qing Geng

Oncology letters 26:420 PubMed37664655

2023

ABCA3, a tumor suppressor gene, inhibits the proliferation, migration and invasion of lung adenocarcinoma by regulating the epithelial‑mesenchymal transition process.

Applications

Unspecified application

Species

Unspecified reactive species

Minglei Song,Liping Gao,Jing Zang,Xiaoying Xing

Frontiers in toxicology 4:840606 PubMed35832493

2022

A New Immortalized Human Alveolar Epithelial Cell Model to Study Lung Injury and Toxicity on a Breathing Lung-On-Chip System.

Applications

Unspecified application

Species

Unspecified reactive species

Arunima Sengupta,Nuria Roldan,Mirjam Kiener,Laurène Froment,Giulia Raggi,Theo Imler,Lea de Maddalena,Aude Rapet,Tobias May,Patrick Carius,Nicole Schneider-Daum,Claus-Michael Lehr,Marianna Kruithof-de Julio,Thomas Geiser,Thomas Michael Marti,Janick D Stucki,Nina Hobi,Olivier T Guenat

Nature cell biology 24:10-23 PubMed34969962

2022

Human alveolar type 2 epithelium transdifferentiates into metaplastic KRT5 basal cells.

Applications

Unspecified application

Species

Unspecified reactive species

Jaymin J Kathiriya,Chaoqun Wang,Minqi Zhou,Alexis Brumwell,Monica Cassandras,Claude Jourdan Le Saux,Max Cohen,Kostantinos-Dionysios Alysandratos,Bruce Wang,Paul Wolters,Michael Matthay,Darrell N Kotton,Harold A Chapman,Tien Peng

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATP-binding cassette sub-family A member 3 antibody

Western blot - Anti-ATP-binding cassette sub-family A member 3 antibody (AB99856)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (6)

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