AB157098

Anti-ATP citrate lyase antibody

Name: Anti-ATP citrate lyase antibody (ab157098) | Abcam
Brand: Abcam Limited
SKU: AB157098

Be the first to review this product! Submit a review

(7 Publications)

Rabbit Polyclonal ATP citrate lyase antibody. Suitable for WB and reacts with Mouse, Human samples. Cited in 7 publications. Immunogen corresponding to Synthetic Peptide within Human ATP-citrate synthase aa 350-400.

View Alternative Names

ATP-citrate synthase, ATP-citrate (pro-S-)-lyase, Citrate cleavage enzyme, ACL, ACLY

2 Images

Unknown

Western blot - Anti-ATP citrate lyase antibody (AB157098)

All lanes:

Western blot - Anti-ATP citrate lyase antibody (ab157098) at 0.4 µg/mL

Lane 1:

NIH3T3 whole cell lysate at 50 µg

Lane 2:

NIH3T3 whole cell lysate at 15 µg

Predicted band size: 120 kDa

true

Exposure time: 10s

Unknown

Western blot - Anti-ATP citrate lyase antibody (AB157098)

All lanes:

Western blot - Anti-ATP citrate lyase antibody (ab157098) at 0.4 µg/mL

Lane 1:

Jurkat whole cell lysate at 50 µg

Lane 2:

Jurkat whole cell lysate at 15 µg

Lane 3:

HeLa whole cell lysate at 50 µg

Lane 4:

293T whole cell lysate at 50 µg

Predicted band size: 120 kDa

true

Exposure time: 30s

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

applications

Immunogen

Synthetic Peptide within Human ATP-citrate synthase aa 350-400. The exact immunogen used to generate this antibody is proprietary information.

P53396

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/5000", "WB-species-notes": "<p></p>" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Caenorhabditis elegans": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chicken": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Gorilla": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Guinea pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rhesus monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Sheep": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Turkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus laevis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Xenopus tropicalis": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Zebrafish": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Storage buffer

pH: 7 - 8 Preservative: 0.09% Sodium azide Constituents: 99% Tris citrate/phosphate

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

ATP citrate lyase (ACL ACLY) is an enzyme responsible for converting citrate and coenzyme A into acetyl-CoA and oxaloacetate using ATP in the process. This reaction is key for lipid and cholesterol biosynthesis. ACLY's alternate name is ATP citrate (pro-S)-lyase and it has a molecular weight of approximately 120 kDa. The enzyme is expressed mainly in the cytoplasm with substantial amounts found in liver and adipose tissues.

Biological function summary

ATP citrate lyase plays an important role in lipid metabolism and energy production. It drives the conversion of citrate-derived acetyl-CoA a central metabolite in lipogenesis providing substrates for fatty acid and cholesterol synthesis. ACLY functions as a homotetramer complex to facilitate its enzymatic activity. This mechanism supports energy homeostasis linking carbohydrate metabolism with lipid biosynthesis.

Pathways

The enzyme ATP citrate lyase is instrumental in the citric acid cycle and fatty acid synthesis pathways. It is a central player in the cholesterol biosynthesis pathway which tightly connects to acetyl-CoA and citrate shuttle processes. In these pathways it interacts functionally with other enzymes such as acetyl-CoA carboxylase which further processes acetyl-CoA into malonyl-CoA serving as a precursor for fatty acid elongation.

ATP citrate lyase becomes significant in metabolic syndrome and cancer. Elevated expression and activity are linked to an increased risk of metabolic diseases including obesity and type 2 diabetes due to its role in excessive lipid accumulation. Moreover cancer cells often exhibit upregulated ACLY activity encouraging tumor growth by supplying acetyl-CoA for lipid biosynthesis. Its activity is intricately linked to proteins such as fatty acid synthase which also contribute to altered lipid profiles in these conditions.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Catalyzes the cleavage of citrate into oxaloacetate and acetyl-CoA, the latter serving as common substrate in multiple biochemical reactions in protein, carbohydrate and lipid metabolism.

See full target information ATP-citrate synthase

style

left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Journal of translational medicine 21:568 PubMed37620891

2023

ACLY as a modulator of liver cell functions and its role in Metabolic Dysfunction-Associated Steatohepatitis.

Applications

Unspecified application

Species

Unspecified reactive species

Paolo Convertini,Anna Santarsiero,Simona Todisco,Michele Gilio,Donatella Palazzo,Ilaria Pappalardo,Dominga Iacobazzi,Maria Frontuto,Vittoria Infantino

Pharmaceutics 15: PubMed37242782

2023

Immunometabolism Modulation by Extracts from Pistachio Stalks Formulated in Phospholipid Vesicles.

Applications

Unspecified application

Species

Unspecified reactive species

Simone Pani,Ilaria Pappalardo,Anna Santarsiero,Antonio Vassallo,Rosa Paola Radice,Giuseppe Martelli,Francesco Siano,Simona Todisco,Paolo Convertini,Carla Caddeo,Vittoria Infantino

Genes 12: PubMed34573442

2021

Mammalian SIRT6 Represses Invasive Cancer Cell Phenotypes through ATP Citrate Lyase (ACLY)-Dependent Histone Acetylation.

Applications

Unspecified application

Species

Unspecified reactive species

Wei Zheng,Luisa Tasselli,Tie-Mei Li,Katrin F Chua

Oxidative medicine and cellular longevity 2021:5533793 PubMed34122722

2021

Phenolic Compounds of Red Wine Modulate the Functional Activity of Macrophages via Inhibition of and the Citrate Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Santarsiero,Paolo Convertini,Antonio Vassallo,Valentina Santoro,Simona Todisco,Dominga Iacobazzi,Yvonne Fondufe-Mittendorf,Giuseppe Martelli,Marcos R de Oliveira,Rosangela Montanaro,Vincenzo Brancaleone,Johannes Stöckl,Vittoria Infantino

Oxidative medicine and cellular longevity 2020:4264815 PubMed33204395

2020

Hydrosol: Untargeted Metabolomic Analysis and Anti-Inflammatory Activity Mediated by NF-B and the Citrate Pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Santarsiero,Alberto Onzo,Raffaella Pascale,Maria Assunta Acquavia,Marianna Coviello,Paolo Convertini,Simona Todisco,Michela Marsico,Corrado Pifano,Patrizia Iannece,Carmine Gaeta,Salvatore D'Angelo,Maria Carmela Padula,Giuliana Bianco,Vittoria Infantino,Giuseppe Martelli

Biochemical and biophysical research communication 529:1117-1123 PubMed32819574

2020

New synthesized polyoxygenated diarylheptanoids suppress lipopolysaccharide-induced neuroinflammation.

Applications

Unspecified application

Species

Unspecified reactive species

Anna Santarsiero,Antonella Bochicchio,Maria Funicello,Paolo Lupattelli,Sabine Choppin,Françoise Colobert,Gilles Hanquet,Lucie Schiavo,Paolo Convertini,Lucia Chiummiento,Vittoria Infantino

Molecules (Basel, Switzerland) 21: PubMed27153053

2016

Soy Isoflavones Regulate Lipid Metabolism through an AKT/mTORC1 Pathway in Diet-Induced Obesity (DIO) Male Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Chao Huang,Dejiang Pang,Qihui Luo,Xiaolin Chen,Qi Gao,Liangqin Shi,Wentao Liu,Yuanfeng Zou,Lixia Li,Zhengli Chen

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-ATP citrate lyase antibody

Western blot - Anti-ATP citrate lyase antibody (AB157098)

Western blot - Anti-ATP citrate lyase antibody (AB157098)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Product promise